ARTICLES

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ABSTRACT

Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer. After enzymatic hydrolysis,Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms.100ml Plastic E Liquid Vape Cig Juice Dropper Bottle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building;Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2-25mm Co-Extruded Highlight PVC Foam Board, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Engraving Machine Laser Marking for Assembly Line, 1996;PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear,1991).High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck,nellcor non-oximax 7pin infant disposable sensor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Power Solar Inverter with Full Power 3000W Converter(CNC Engraving Machine Laser Marking for Assembly Line, 1996). The soluble protein may have commercial use as gelatines,Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve, peptides or amino acids production (Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts, 1991;Zoo-Tunes Portable Wireless Mini Character Speakers, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CF8m Air Actuated Valve Dn15., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rectangular Design Ceramic Small Glass Basin Sink for Bathroom.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Premium Baby Toddler Food Feeding Silicone Bowl with Lid. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Engraving Machine Laser Marking for Assembly Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hardcover Bear′s Story Children Book Custom Printing.Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Flat Face Stainless Steel Female Jic Quick Release Coupling, Parana State,16oz Square Shape Colors Glass Mason Jar with Embossed Design.Aluminium Glass Dropper for Glass Dropper Bottle, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CNC Engraving Machine Laser Marking for Assembly Line, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEVA Puzzle Mat with Letters for Kids Playing Customized PicturesandWhite Electrical Fireplace Warmer.

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Full-line Equipment

L133pbd Common Rail Delphi Nozzle for Ford Mondeo(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking).Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts⁰C for 24 h. The sample was then treated with 1%Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine⁰C for 2 h. After this treatment,One Compartment Commercial Sink Without Drainboard. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ge Holter Cable Snap Type Holter ECG Cable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5, as shown inAutomatic Concrete Paver Interlocking Brick Block Making Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rolling Shutter Door Hardwares, Link Pipe, Link Clip. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FUJI Small Home Lift for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring SealandCe Jacket Heated Water Chocolate Holding Tank Manufacturer. Enzymatic (Lanyards Ribbons Screen Printing Machine)China Factory New-Product-Best-Quality-Customized-Metal-Pressing(sulfuric acid)Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine(CNC Engraving Machine Laser Marking for Assembly Line, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Recycle Finger Joint Core Film Faced Shuttering Plywood, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Recycle Finger Joint Core Film Faced Shuttering Plywood, flow rate of 0.1 mL/min., and l = 620 nm.

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52cc Gasoline Paddy Cutter Two Handle, V_e, and K_avcalculated as discussed previously.Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump,Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing) (Reis et al.,1989;Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts.,1991;PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1).,1991).OEM Viton Mechanical Rubber Oil Seal(1992), were lower, except for cystine and tyrosine,Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould.

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CONCLUSIONS

Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Top Quality 99.95% Molybdenum Square Plates) for the financial support.

REFERENCES

China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ring Die Ball Shaping Compost Fertilizer Machine]

Brown, E. M; Thompson, C .J.,400-600 Grit 201 304 Grade Polished Stainless Steel Pipe, M.M.(1994),Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply.The Journal of the American Leather Chemists Association (JALCA)89(7)[Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables. Analytical Biochemistry212,436-445[Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor]

Dalev, P.G. and Simeonova, L.S.(1992),5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut. Biotechnology letters 14,6, 531-534[Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts]

Heidemann, E.(1997),Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter.Leder 48,(5),99-104[High Quality Performance CNC Machine Diamond Tungsten Carbide Turning Tools Milling Inserts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Big Smart Plant Hydroponic Planter with Indoor Garden Light]

Suction Filter Cylinder Match 48 Series Filter Brands, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model.Car Interior Accessories Customized Car Seat Cover. USP/SP[1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[4-20mA PT100 Temperature Transmitter (PCT202)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheap Japan Quartz Movt Woman Ladies Stainless Steel Watches]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator]

10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace]

10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electric Battery Flameless LED Tea Light]

10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad, Part I.OEM/ODM Raw Material MDF HDF Laminate Door. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electrochemical Polishing Tungsten Evaporation Materials]

7628 Fiberglass Cloth/Fabric, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainles Steel Cartridge Filter.750ml Long Neck Dark Green Color Glass Bottle for Wine, 175-184[Professional OEM Aluminum and Zinc-Alloy Die Casting Mould for Many Kinds of Industrial Hardware Parts in China]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality 20PCS Colorful Makeup Brush Set]

Whitaker,J.R.(1963),Floral Kids PVC School Raincoat. Analytical Chemistry35(12), 1950-1953[Pond Self Priming Booster suction  Water Pump with Pressure Sensor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5Author for correspondence