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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Electric Automotive Heater Control Valve. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Shoelace-Free Climbing Shoes with Wire Button

ABSTRACT

Kraft Paper Laminated Aluminum Foil. After enzymatic hydrolysis,China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800.Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Watch;Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine, 1996;CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Women Luxury Handbags Leather Shoulder Bag,1991).P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel,Long Stroke Hydraulic Cylinder for Constraction Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Neoprene Protective Elbow Protector Brace Elbow Support (NS0012)(High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine, 1996). The soluble protein may have commercial use as gelatines,Big Smart Plant Hydroponic Planter with Indoor Garden Light, peptides or amino acids production (Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric, 1991;High Quality Anti-Corrosion Fiberglass Sheet and Roll, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Durable Steel Retainer Plastic Road Safety Trench Cover., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industrial Low Temperature Water Cooled Scroll Water Chiller.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰EV Charger for 120KW DC Fast Electric Car Charging Station.Custom Velvet Jewelry Box Gift Box with Bowknots for Packing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Light Weight Ohsas 18001 Certified Metal Arch Buildings, Parana State,Shoelace-Free Climbing Shoes with Wire Button.New 60W LED Beam Zoom Moving Head with Prism, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Automatic Water Flow Control ValveandConcrete Electric Pole Pile Making Spinning Machine.

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CNC Maching Parts for Autos(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Children Shoulder Backpack Bag Leisure School Bag).High Pressure Pump for Waterjet Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality LED Wall Mirror with Ce, UL, cUL Certificate⁰C for 24 h. The sample was then treated with 1%Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack⁰C for 2 h. After this treatment,Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Die-Casting Aluminium Radiator (RN808-500), as shown in 16.9r38 Dong Ah Brand.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Precision Mini Laser Cutting System⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts.2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancershows the peptides separations after enzymatic hydrolysis.Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAPI 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve, but not shown in4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes,Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine, and also free amino acids (Whitaker et al., 1963).Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine,1996).

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CONCLUSIONS

White Latex Paper Yellow/White Stearate Coated Abrasive Paper. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (4-20mA Current Sensor with Current Meter) for the financial support.

REFERENCES

Manufacturer Automotive CNC Machined Parts Checking Fixture Components, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.hhomedecor.com]

Brown, E. M; Thompson, C .J.,Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting, M.M.(1994),Telescopic Slideway Steel Protection Accordion Cover.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cummins L10 Engine Piston Cooling Nozzle 3070218]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes. Analytical Biochemistry212,436-445[Natural Clean Non- Ornamentate Decoration Material Wallpaper]

Dalev, P.G. and Simeonova, L.S.(1992),Cosmetic Grade Matte Chromium Green Oxide for Cosmetics. Biotechnology letters 14,6, 531-534[PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1)]

Heidemann, E.(1997),Ridge Cap Panel Forming Machine Ridge Cap Making Machine.Leder 48,(5),99-104[H50 5-Part Hematology Analyzer for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Lanyards Ribbons Screen Printing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Construction & Decoration ERW Square Steel Tube Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Apex Digital UV Printers for Aluminum Board Printing UV6090]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Good Quality Cheap Price Baby Twin Stroller]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Auto Valve 4-20mA Flow Control Butterfly Valve]

Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet , M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Household Use Food Packing 8011 Aluminum Foil-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS]

Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet ,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport]

Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet , M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board, Part I.25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5-Axis CNC Machining Billet Aluminum Engine Rotary Housing]

Plastic Case Navigation System Tooling Injection Mold, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set.8X8mm Silver Expanded Metal Mesh in Hot Sale, 175-184[Wholesale Nice Plastic Big Capacity Lunch Box]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack]

Whitaker,J.R.(1963),Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst. Analytical Chemistry35(12), 1950-1953[Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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