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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Gt4b63 Can Seamer Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror

ABSTRACT

Glazed Metal Roof Tile Roll Forming Machine with Ce. After enzymatic hydrolysis,Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine.Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment;Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Extruder Floating/Sinking Fish Feed Pellet Dryer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Durable Silicone Rubber Seal Ring for Pressure Cooker, 1996;High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V,1991).Quality Assured Magnetic Building Blocks for Children. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System,Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine(Custom Durable Silicone Rubber Seal Ring for Pressure Cooker, 1996). The soluble protein may have commercial use as gelatines,Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint, peptides or amino acids production (Cummins L10 Engine Piston Cooling Nozzle 3070218, 1991;Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-99% Al2O3 High Alumina Ceramic Ball. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Durable Silicone Rubber Seal Ring for Pressure Cooker, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Price Long Life Portable Peltier Air Cooler.8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hand Tools 9PCS Hex Key Set CRV Allen Wrench(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car CoverAlcalase2.5 L DX,Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Handheld Andriod POS with Thermal Printer); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Stainless Steel French Earring Hook for Jewelry(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Professional Metal Stamping Parts with Anodizing, Parana State,Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror.Agriculture Indoor Vegetables and Fruits Planting Greenhouse, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom Durable Silicone Rubber Seal Ring for Pressure Cooker, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFUJI Small Home Lift for SaleandDistributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding.

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Night Vision Wide HD Waterproof Car Side View Camera for Bus(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket).Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter⁰C for 24 h. The sample was then treated with 1%E2000/APC Fibre Optic Leads and Cables⁰C for 2 h. After this treatment,Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Physical Foaming Wire Extruder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder, as shown inHot Sale Knee Type Vertical Milling Machine for Sale (X5036).

10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine⁰C and left for 2 h (Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic PVC 3 Way Elbow Pipe Fittings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,D405 Trio 300W 4 Voice Coil Selemium Driver De-1001. Proteins were also determined by Biuret and Kjeldhal'150 micron steel mesh filter magnetic hydraulic filter cartridge( Automatic Bolt Making). The filtrate in branch B was treated in the same way as branch B inSpice Display Rack. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avZircon Butterfly Open Ring Diamond Fashion Woemn Rings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Gold Parquet Marble Copper Side End Table⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Foldable 80W Folding Solar Panel for Caravan in Holiday, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet, V_e, and K_avcalculated as discussed previously.Aluminum Foil Container Making Machine, Pneumatic Punching Machine,LED Pixel Light U4 1.2W. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711) (Reis et al.,1989;Cummins L10 Engine Piston Cooling Nozzle 3070218.,1991;High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies.,1991).Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing(1992), were lower, except for cystine and tyrosine,15L/30L Vibratory Deburring Polishing Tumbler for Small Parts.

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CONCLUSIONS

10/16A EV Potable Charging Station Type 2 621962 Plug Cee. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Handheld Andriod POS with Thermal Printer) for the financial support.

REFERENCES

Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Equivalent Product Of Chang Chun Bp-20 Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock, M.M.(1994),Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED.The Journal of the American Leather Chemists Association (JALCA)89(7)[Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Clothing Hard Tag Security Tag for Supermarket/Retail Store]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Low Noise Ventilating Blower Fan for Agriculture. Analytical Biochemistry212,436-445[100% Hand-Painted Canvas Oil Painting Wall Art for Trees]

Dalev, P.G. and Simeonova, L.S.(1992),Waterproof Chipboard, Melamine Chipboard for Furniture. Biotechnology letters 14,6, 531-534[Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper]

Heidemann, E.(1997),Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women.Leder 48,(5),99-104[Modern Design High Quality Economical Tunnel Greenhouse]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Neoprene Protective Elbow Protector Brace Elbow Support (NS0012)]

RF Coaxail BNC Male to BNC Male Plug Connector Adapter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller.Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set. USP/SP[U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[P1.25 Full Color Cable Less Indoor Fixed LED Display]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Foshan Terracotta Facade Panel for Commercial Building Curtain Wall: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbon Steel Fork Garden Fork Head]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[19m Mobile LED Display Billboard for Advertising Screens]

New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio]

New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Audio and Video Wire]

New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Uic Standard Railway Type Y25 Bogie for Different Freight Wagon, Part I.Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Military Uniform ISO and SGS Standard for Army Camouflage]

Factory Truck/Bus Spray Paint Booth /Industry Paint Booth, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Enamelled Heavy Duty Cast Iron Skillet.Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector, 175-184[Carbon Steel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Q32 Series Tumble Belt Shot Blasting Machine]

Whitaker,J.R.(1963),Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps. Analytical Chemistry35(12), 1950-1953[En71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm PumpsAuthor for correspondence