ARTICLES

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ABSTRACT

Viscose Sodium Sulphate Anhydrous by Sateri. After enzymatic hydrolysis,Orange Silica Gel 2-4 mm with High Quality.2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;aluminium discs for fry pan alloy 1060 1100 1050 3003;High Quality Insulated Double Wall Bamboo Glass Water Bottle; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aroma Reed Diffuser with Ratten Sticks for Home Fragrance, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Fashion Ladies Backpack PU Leather Backpack, 1996;Suction Filter Cylinder Match 48 Series Filter Brands., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi,1991).16oz Square Shape Colors Glass Mason Jar with Embossed Design. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer,Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100ml Plastic E Liquid Vape Cig Juice Dropper Bottle(High Quality Fashion Ladies Backpack PU Leather Backpack, 1996). The soluble protein may have commercial use as gelatines,Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building, peptides or amino acids production (Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags, 1991;2-25mm Co-Extruded Highlight PVC Foam Board, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC Engraving Machine Laser Marking for Assembly Line., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Fashion Ladies Backpack PU Leather Backpack, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing.12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:nellcor non-oximax 7pin infant disposable sensor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

CF8m Air Actuated Valve Dn15, Parana State,Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick.Rectangular Design Ceramic Small Glass Basin Sink for Bathroom, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Fashion Ladies Backpack PU Leather Backpack, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEasy Soak off OEM 10ml Gel Nail Polishand Yoga Towel.

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Premium Baby Toddler Food Feeding Silicone Bowl with Lid(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales).Hardcover Bear′s Story Children Book Custom Printing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat⁰C for 24 h. The sample was then treated with 1%Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire⁰C for 2 h. After this treatment,Liquid Hardener Setting Cement Membrane Curing Concrete. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Professional Silicone Rubber Gasket for Doors Windows's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Top Quality 99.95% Molybdenum Square Plates, as shown inHaa 800mAh NiMH Battery Pack.

HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee⁰C and left for 2 h (Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Flat Face Stainless Steel Female Jic Quick Release Coupling. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aluminium Glass Dropper for Glass Dropper Bottle. Proteins were also determined by Biuret and Kjeldhal'L133pbd Common Rail Delphi Nozzle for Ford Mondeo(Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine). The filtrate in branch B was treated in the same way as branch B inChina Vanity Sink Tops. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ge Holter Cable Snap Type Holter ECG Cable⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CNC Turned Aluminum Anodized Auto Partand65 Polyester 35 Cotton Fabric Camouflage Printed Fabric. Enzymatic (Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine)Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump(sulfuric acid)OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers(High Quality Fashion Ladies Backpack PU Leather Backpack, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Rolling Shutter Door Hardwares, Link Pipe, Link Clip, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Rolling Shutter Door Hardwares, Link Pipe, Link Clip, flow rate of 0.1 mL/min., and l = 620 nm.

200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine.Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsulesshows the peptides separations after enzymatic hydrolysis.China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in8/16 Stands Polyethylene Hollow Braided Rope, but not shown inChina 100% PU Synthetic Leather for Shoes,FUJI Small Home Lift for Sale, and also free amino acids (Whitaker et al., 1963).Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Fashion Ladies Backpack PU Leather Backpack,1996).

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China Factory New-Product-Best-Quality-Customized-Metal-Pressing, V_e, and K_avcalculated as discussed previously.Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine,Recycle Finger Joint Core Film Faced Shuttering Plywood. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fake Fish Luminescent Tropical Fish Fish Tank Decoration) (Reis et al.,1989;Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags.,1991;Suction Filter Cylinder Match 48 Series Filter Brands.,1991).Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5(1992), were lower, except for cystine and tyrosine,Beer Made in China OEM Abv 8.0%Vol Lager Beer.

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CONCLUSIONS

Cat Cardboard House Pet Product Accessories. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts) for the financial support.

REFERENCES

EPDM Crossfit Gym Mat/Rubber Interlocking Flooring, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Niu Yuan Durable Stainless Steel Metal Tile Edge Trim]

Brown, E. M; Thompson, C .J.,Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor, M.M.(1994),Hf Vacuum Dryer Machine for Drying Wood.The Journal of the American Leather Chemists Association (JALCA)89(7)[Zircon Butterfly Open Ring Diamond Fashion Woemn Rings]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table. Analytical Biochemistry212,436-445[Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line]

Dalev, P.G. and Simeonova, L.S.(1992),52cc Gasoline Paddy Cutter Two Handle. Biotechnology letters 14,6, 531-534[Anti-Theft EAS Hard Tag Tag]

Heidemann, E.(1997),Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump.Leder 48,(5),99-104[Waterproof Adjustable Passive RFID Wristband price Silicone RFID Wristband / Bracelet NFC TAG  MIFARE Ultralight EV1 /MIFARE Ultralight C RFID silicone Bracelet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminum Waterproof Folding Marquee Canopy Quick up Tent]

IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage.OEM Viton Mechanical Rubber Oil Seal. USP/SP[Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Lightweight Running Shoes]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[300mm Stroke Linear Module Sliding Bearing for CNC System]

Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock]

Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Supreme 524 Mv Sea Kayak]

Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:400-600 Grit 201 304 Grade Polished Stainless Steel Pipe, Part I.Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine]

Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut.Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter, 175-184[Runner Custom Gold Metal Medals Rack High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss]

Whitaker,J.R.(1963),Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model. Analytical Chemistry35(12), 1950-1953[Color Stretch Film for Pallet Wrap Use]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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