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ABSTRACT

Women Luxury Handbags Leather Shoulder Bag. After enzymatic hydrolysis,P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop.Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Long Stroke Hydraulic Cylinder for Constraction Machine;Neoprene Protective Elbow Protector Brace Elbow Support (NS0012); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Big Smart Plant Hydroponic Planter with Indoor Garden Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric, 1996;High Quality Anti-Corrosion Fiberglass Sheet and Roll., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Durable Steel Retainer Plastic Road Safety Trench Cover,1991).Industrial Low Temperature Water Cooled Scroll Water Chiller. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134),EV Charger for 120KW DC Fast Electric Car Charging Station,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Velvet Jewelry Box Gift Box with Bowknots for Packing(Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric, 1996). The soluble protein may have commercial use as gelatines,Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush, peptides or amino acids production (Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode, 1991;Aluminum Waterproof Folding Marquee Canopy Quick up Tent, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4-20mA Current Sensor with Current Meter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Tc Made Boy′s Short Pants.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Light Weight Ohsas 18001 Certified Metal Arch Buildings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New 60W LED Beam Zoom Moving Head with Prism.CNC Maching Parts for Autos-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Pressure Pump for Waterjet Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

High Quality LED Wall Mirror with Ce, UL, cUL CertificateAlcalase2.5 L DX,Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Die-Casting Aluminium Radiator (RN808-500), Parana State,CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal.Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Precision iPhone Cases Fiber Laser Marking MachineandTse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine.

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Wholecut Oxford Plain Toe Black Antique Men Shoes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box).New Technology Carbon Cleaning Tools Auto Accessories(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Environmental Test Salt Spray Tester Price⁰C for 24 h. The sample was then treated with 1%Custom 2 Ring Binder PP Lever Arch Clip File Folder⁰C for 2 h. After this treatment,Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Laser Customized Telescopic Steel Cover, as shown inAutomatic China Beef Cattle Sheep Tripe Washer Cleaning Machine.

High Precision Mini Laser Cutting System⁰C and left for 2 h (Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195). Proteins were also determined by Biuret and Kjeldhal'One to One Order Following Strong Real Gold Thread(Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply). The filtrate in branch B was treated in the same way as branch B inPlastico Reciclaje Maquina/Maquina De Reciclaje De Plastico/Plastic Recycling Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New Design Paper Pyramid Memo Cube with Ribbon for Promotion.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Medallion, Plastic Ceiling Rose. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPP PE PVC Single Wall Corrugated Pipe Making Machine Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern FurnitureandWood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile. Enzymatic (Centrifugal Casting Stainless Steel Furnace Tube)Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System(sulfuric acid)Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting(Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel Automatic Water Flow Control Valve, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel Automatic Water Flow Control Valve, flow rate of 0.1 mL/min., and l = 620 nm.

Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0.Spiral Rib Diesshows the peptides separations after enzymatic hydrolysis.Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHuman Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression, but not shown inLayflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch,3phase DC to AC Variable Frequency Drive Water Solar PV Inverter, and also free amino acids (Whitaker et al., 1963).Quality Printed Cigarette Tipping Paper. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric,1996).

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Telescopic Slideway Steel Protection Accordion Cover, V_e, and K_avcalculated as discussed previously.Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes,Cosmetic Grade Matte Chromium Green Oxide for Cosmetics. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Model Water Source Heat Pump (SLW100D)) (Reis et al.,1989;Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode.,1991;High Quality Anti-Corrosion Fiberglass Sheet and Roll.,1991).Ridge Cap Panel Forming Machine Ridge Cap Making Machine(1992), were lower, except for cystine and tyrosine,Grinding Factory Corn Maize Flour Milling Mill Making Machines.

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CONCLUSIONS

Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag) for the financial support.

REFERENCES

Construction & Decoration ERW Square Steel Tube Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[as-Mc Fabric Strawberry Potato Felt Plant Planter Black Grow Bags]

Brown, E. M; Thompson, C .J.,Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet , M.M.(1994),Household Use Food Packing 8011 Aluminum Foil.The Journal of the American Leather Chemists Association (JALCA)89(7)[CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board. Analytical Biochemistry212,436-445[Men Genuine Leather Sling Shoulder Satchel Messenger Bag]

Dalev, P.G. and Simeonova, L.S.(1992),25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale. Biotechnology letters 14,6, 531-534[Community IC Access Card Induction ID Time Card Smart Card]

Heidemann, E.(1997),Plastic Case Navigation System Tooling Injection Mold.Leder 48,(5),99-104[Craft and Powerful Cylinder NdFeB Magnets]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Waste Paper Stripper]

150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set.8X8mm Silver Expanded Metal Mesh in Hot Sale. USP/SP[Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Extruder Floating/Sinking Fish Feed Pellet Dryer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Qsb 6.7 Diesel Engine Crankshaft for Excavator Heavy Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine]

100% Durable Flooring Roll for Hotel Office School and Health Project Floor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manufacturers in China Ceramic Injection Moulding Machine]

100% Durable Flooring Roll for Hotel Office School and Health Project Floor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia]

100% Durable Flooring Roll for Hotel Office School and Health Project Floor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pet Bottle Washing Recycling Line in China, Part I.Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Porosity Zirconia Zro2 Foam Filter for Foundry]

Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event.Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress, 175-184[m.shiningstar-world.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Gt4b63 Can Seamer Machine]

Whitaker,J.R.(1963),Sc/APC Fiber Optic Fast Hot Melt Connector. Analytical Chemistry35(12), 1950-1953[Cat Cardboard House Pet Product Accessories]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Professional Family Nonstick Cast Iron Frying PanAuthor for correspondence