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ABSTRACT

HDPE Pipe for Water Supply (OD: 16mm-800mm). After enzymatic hydrolysis,Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs.2019 Male & Female Stainless Steel Quick Coupling. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board, 1996;FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Colorful Asphalt Shingle for Roof Water Proof,1991).Durable Inline Hockey Skate Puck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane),18mm Thickness Best Quality Poplar Birch Commercial Plywood,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag(Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board, 1996). The soluble protein may have commercial use as gelatines,Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box, peptides or amino acids production (Nylon Hammer Fixings Drive Anchor, 1991;OEM Electrolyte Effervescent Tablet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant.304 321 310 310S Welded Decorative Stainless Steel Pipe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Aluminum Plate Fin Heat Exchanger/Oil Cooler Core(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Nylon Hammer Fixings Drive Anchor).3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet⁰C for 24 h. The sample was then treated with 1%5-Axis CNC Machining Billet Aluminum Engine Rotary Housing⁰C for 2 h. After this treatment,EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Clamshell Book Shape Black Cardboard Magnet Gift Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashionable Simple Printing Mini Key & Coin Purse, as shown inClass 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications.

CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch⁰C and left for 2 h (PCBA SMT Equipment PCB Depaneling Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Kids Folding Play Mat Kids Play Room Floor Mat. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Roller Window Shutter for Residential Usage. Proteins were also determined by Biuret and Kjeldhal'Pre-Seasoned Cast Iron 5-Piece Cookware Set(Digital Control with LED Display DC Solar Small Deep Freezer Fridge). The filtrate in branch B was treated in the same way as branch B in809 Ball Bearing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHydraulic Hose SAE 100 R1AT(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ts-Micro Portable Defense Loudspeaker System, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Plastic Ceiling RoseandWaterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm. Enzymatic (Medical Supply for Laparoscopic Instrument (MIM Straight Scissors))86bl3a125 1.65A 660W 310V BLDC(sulfuric acid)Tamper Evident Branded Printed Packing Pet Adhesive Security Tape(Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle, flow rate of 0.1 mL/min., and l = 620 nm.

Hotel Use and Steel Material Poultry House for 10000 Chickens.Flip PU Tablet Case Cover for iPad iPad Mini 4 Covershows the peptides separations after enzymatic hydrolysis.50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Clamshell Book Shape Black Cardboard Magnet Gift Box, but not shown inOptical Fiber Connector Cleaning Cassette Reel Cleaner,Kontron One-Piece 10-Lead EKG Cable with Resisitance, and also free amino acids (Whitaker et al., 1963).Best Price of Peanut Digger / Groundnut Harvester in Australian Africa. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board,1996).

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CONCLUSIONS

Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar) for the financial support.

REFERENCES

Ce Certificate High Security Steel Shackle Padlock, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[jinbinvalve]

Brown, E. M; Thompson, C .J.,Drive Shaft for Jmc 8-94134458-PT, M.M.(1994),DSG Ball Screw Fixed Seat Nut Support.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Watch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car. Analytical Biochemistry212,436-445[Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker]

Dalev, P.G. and Simeonova, L.S.(1992),Apex Digital UV Printers for Aluminum Board Printing UV6090. Biotechnology letters 14,6, 531-534[Decoration Sign Green Grey White PVC Foam Board]

Heidemann, E.(1997),24 Molds Commercial Ice Cream Cone Maker.Leder 48,(5),99-104[Disposable RFID Paper Card for Exhibition and Trade Show Ticket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company]

Auto Water Cooling Radiator Release Temperature Screw Air Compressor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine.Fashion Children Fur Pompom Beanie Scarf Knit Set. USP/SP[Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[FUJI Small Home Lift for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Steel Jacket Steam Insulation Pipeline Production Best Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Green Agricultural Silage Wrap Film]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater]

Haa 800mAh NiMH Battery Pack, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket]

Haa 800mAh NiMH Battery Pack,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Knee Type Vertical Milling Machine for Sale (X5036)]

Haa 800mAh NiMH Battery Pack, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts, Part I.Electronic Muscle Stimulator EMS Machine for Slimming. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing]

Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover.Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber, 175-184[100% Natural Konjac Sponge with 9 Colors]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Automatic Bolt Making]

Whitaker,J.R.(1963),Custom Precision Aluminum Die Casting for Bicycle Accessories. Analytical Chemistry35(12), 1950-1953[ Melamine Cabinet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball ValveAuthor for correspondence