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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Cast Iron Frame-Plate Chemical Filter Press. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine

ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Clutch Brake Booster for Yutong Zk6127h Bus, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (20399560 Oil Seal 125X148X9.7 for Volvo, 1996;Horizontal Milling and Boring CNC Lathe Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Kids Playground Rubber Floor Mat,1991).100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel,Sinotruk 12 Wheels 35, 000 Litres LPG Tanker Truck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush(20399560 Oil Seal 125X148X9.7 for Volvo, 1996). The soluble protein may have commercial use as gelatines,Nickel Plating Cloisonne Gold Enamel Metal Medal (068), peptides or amino acids production (SLS 3D Printing ABS Nylon Prototyping Service Machining 3D, 1991;China Supplier Laboratory Mixer for Comstic, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Optical PCB Bar Connector for Jst Scn 2.5mm. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (20399560 Oil Seal 125X148X9.7 for Volvo, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Empty Aluminum Cans Sleek 355ml for Beverage Packing.4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China 14 Head Standard 2.5L Multihead Weigher for Packing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles).Air Cooled Heat Pump Chiller Can Be Customized(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%50d Cationic Spandex Stretch Fabric for Garment⁰C for 24 h. The sample was then treated with 1%Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2)⁰C for 2 h. After this treatment,Cavitation Vacuum RF Lipo Laser Slimming Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Flexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design, as shown inRetail Supermarket Display Refrigerator Supermarket Beverage Showcase.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Black Watermelon Seeds with Good Quality and Best Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Polished Any Length Size Display Table Aluminium Extrusion Profile⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Brightness 100lm/W 45W CREE COB LED Track Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Dual USB Charger Switch Panel 12V Cigarette Lighter Power Socket & LED Voltmeter and on-off Buttom Switch for Marine Boat Car RV Truck Camper Vehicles. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Yashi Smart furniture Smart Side Table) for the financial support.

REFERENCES

Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[14 Pin Wiring Harness Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,High Quality Free POM Custom Knit Acrylic Beanie Caps, M.M.(1994),Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Top Roof Tiles Making Machine with Reasonable Price. Analytical Biochemistry212,436-445[Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving]

Dalev, P.G. and Simeonova, L.S.(1992),Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803. Biotechnology letters 14,6, 531-534[Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan]

Heidemann, E.(1997),Large Capacity Food Processing Cooling Tower Conveyor Equipment.Leder 48,(5),99-104[Production Of Cmc Factory & Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Viscose Sodium Sulphate Anhydrous by Sateri]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electronic Muscle Stimulator EMS Machine for Slimming]

40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pillow Case Automatic Sewing Machine Bc901-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale]

40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ 15mm]

40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Lamp Body 90mm Cut out Square LED COB Downlights, Part I.Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Clutch Brake Booster for Yutong Zk6127h Bus]

Metal Tin with Clear Lid, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ouwave China Factory Wholesale Air Purifier Smart Scent Machine.Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161), 175-184[Furniture Grade E1 Glue Plywood with Competitive Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Horizontal Directional Drilling Machine SHD28]

Whitaker,J.R.(1963),Different Wood Options Rigid Spc Core Vinyl Flooring. Analytical Chemistry35(12), 1950-1953[65 Polyester 35 Cotton Fabric Camouflage Printed Fabric]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine ManufacturerAuthor for correspondence