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ABSTRACT

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Key words;Multifunction Copper Busbar Processing for Aluminum Material;Men Genuine Leather Sling Shoulder Satchel Messenger Bag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electric DC 24V Geared Motor for Auto Doors, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided, 1996;RF Shielding MRI Door with Brass Frame for RF Cage., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Geotextile Beg Used for Beach Restoration Geobag,1991).Reusable Silicone Food Storage Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dental Emax Empress Crown From China Dental Lab,Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer(Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided, 1996). The soluble protein may have commercial use as gelatines,Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill, peptides or amino acids production (Dubai Products Rectified Tile for Wall, 1991;ANSI B16.5 Stainless Steel SS316 Forging Blind Flange, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Integrated Hydraulic Steering Control Units (BZZ1F SCU). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Model Water Source Heat Pump (SLW100D).Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Red Papillon (JSY-3) Satin Ribbon Flower⁰C and left for 2 h (Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Steel Structure Construction Building Metal House. Proteins were also determined by Biuret and Kjeldhal'1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting(OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers). The filtrate in branch B was treated in the same way as branch B inUV Curing Machine for UV Paint. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Sanitary Thread Pneumatic Butterfly Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Wholesale Reach Standard Home and Office Furniture Fitting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale Energy Saving Induction Heater for Billet Making, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

8FT Wood Log Debarker Machine for Plywood Machinery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Capacity Vibrating Screen Manufacturer) for the financial support.

REFERENCES

Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Car Key Duplicating Machine]

Brown, E. M; Thompson, C .J.,Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine, M.M.(1994),High Density Graphite Crucible for Melting Metal.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Manufacture Table Type CNC Horizontal Boring Milling Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Price 201 304 Stainless Steel Coils]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hydraulic Press Corrugated Cold Roll Automatically Forming Machine. Analytical Biochemistry212,436-445[High Pressure Pump for Waterjet Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine. Biotechnology letters 14,6, 531-534[Hot-Sale Products GRP Tower for New Energy Industry]

Heidemann, E.(1997),Medical Supplier Non Woven 3ply Earloop Disposable Face Mask.Leder 48,(5),99-104[36W Emergency Lighting LED Grow Lights LED Panel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Healthy Grooming Kit for Caring Newborn Baby]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Frequency Online UPS W/3W-20K]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sunflower Seeds Packing Machine for Packaging Sugar]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag]

LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Sanitary Thread Pneumatic Butterfly Valve]

LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ 750mm Cuttable Capacitive Diesel Fuel Level Sensor]

LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign, Part I.Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100% Polyester Luxury Bedding Comforter Fleece Blanket Throw]

Whitaker,J.R.(1963),Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater. Analytical Chemistry35(12), 1950-1953[Different Wood Options Rigid Spc Core Vinyl Flooring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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