ARTICLES

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ABSTRACT

New Design Disposable Trocars for Laparoscopic Surgery. After enzymatic hydrolysis,Disposable Medical Combined Bacterial Viral Hme Filter.Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System;3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Efficiency Pineapple Canning Plant, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Embossed Pattern PU Leather Classic Mens Wallet, 1996;Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Casting PE Film with Ash for Adult Diaper Back Sheet,1991).Supra Brand Laundry Liquid Baby Formula. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger,Cleaning Products Sponge Vacuumized Pack 65mm,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mineral Water Bottle Mould Plastic Blowing Mold(Embossed Pattern PU Leather Classic Mens Wallet, 1996). The soluble protein may have commercial use as gelatines,High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards, peptides or amino acids production (6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine, 1991;Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cookies Cake Automatic Rotary Packing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Building Material Quick Install Types of Scaffolding Couplers.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Embossed Pattern PU Leather Classic Mens Wallet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic.Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cheap Topmac Brand Mobile Concrete Mixing Plant, Parana State,CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery.Color Stretch Film for Pallet Wrap Use, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Embossed Pattern PU Leather Classic Mens Wallet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLow Price Fashion Unique Plastic ZipperandZoo-Tunes Portable Wireless Mini Character Speakers.

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Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Fashion Women Clothes Fashion Yaga Pants).Hemp Recycle Polyester Blenched Jersey Knit Fabric(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV⁰C for 24 h. The sample was then treated with 1%Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule⁰C for 2 h. After this treatment,3D Creative Design Metal Medal Promotion Gift of Sport. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe, as shown inHigh Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair.

Composite Plate Dedicated Drop Hose Gear Metering Pump Price⁰C and left for 2 h (HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Brake Fluid Tank Container Hot Plate Plastic Welding Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices. Proteins were also determined by Biuret and Kjeldhal'Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish(Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning). The filtrate in branch B was treated in the same way as branch B in10w Led Angel Eyes E39. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPuffed Core Filling Sanck Production Machine with Ce From China(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cummins Fuel Injector Kit (3076736 3095023)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

HDPE Large Diameter Hollowness Wall Winding Pipe Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mini Succulent Planters, Cactus Pots with Wooden Cement Pot) for the financial support.

REFERENCES

Non-Woven Medical Outside Earloop Mask Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[V-Shape Lifting Device For Neck Care]

Brown, E. M; Thompson, C .J.,Professional Family Nonstick Cast Iron Frying Pan, M.M.(1994),Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Union NPT Bsp BSPT Stainless Steel Pipe Fittings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashionable Clothing Garment Paper Swing Tags (ST-01). Analytical Biochemistry212,436-445[PE Plastic Road Safety Barricade Caution Tape]

Dalev, P.G. and Simeonova, L.S.(1992),Horizontal Directional Drilling Machine SHD28. Biotechnology letters 14,6, 531-534[Glass Board Automatic Sloping Printing Table]

Heidemann, E.(1997),Electrial Galvanized Conduit Box/Gi Pipe Fitting.Leder 48,(5),99-104[China Manufacturer Aluminum Sheet Metal Fabrication]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Medical Supply for Laparoscopic Instrument (MIM Straight Scissors)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[99% Purity Caustic Soda Flakes From Direct Manufacture on Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[150kw Plastic Bottle Recycling Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Airtight Cap Microwavble High Borosilicate Glass Food Container]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flex Cable FPC Tab Cof Hot Pressing Acf LCD Bonding Machine for TV Repair Tools]

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High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card]

High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:TV Stand Carving European LED Lights Heating Fireplace (320SS), Part I.High Quality Mini LED Flood Light SMD Flood Light 150W. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cast Iron Frame-Plate Chemical Filter Press]

Low Price Fashion Unique Plastic Zipper, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Silica Gel- Indicating Blue to Pink After Adsorption.Offer High Quality Square and Round Cast Iron Manhole Cover, 175-184[Custom Print Paper Cardboard Flat Folding Black Magnetic Gift Box]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill]

Whitaker,J.R.(1963),White Tight Long Sleeves off Shoulders Celebration Bandage Dress. Analytical Chemistry35(12), 1950-1953[Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Supplier Laboratory Mixer for ComsticAuthor for correspondence