ARTICLES

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children playgroundEdivaldo E.Garcia^I; Cláudia C.M.Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Motar Plastering Machine with Screw Pump}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-OEM ODM Aluminum Casting Parts. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Pressure Low Volume 2900rpm electric Water Pump

ABSTRACT

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Key words;Multifunction Copper Busbar Processing for Aluminum Material;Men Genuine Leather Sling Shoulder Satchel Messenger Bag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electric DC 24V Geared Motor for Auto Doors, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided, 1996;RF Shielding MRI Door with Brass Frame for RF Cage., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Geotextile Beg Used for Beach Restoration Geobag,1991).Reusable Silicone Food Storage Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dental Emax Empress Crown From China Dental Lab,Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer(Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided, 1996). The soluble protein may have commercial use as gelatines,Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill, peptides or amino acids production (Dubai Products Rectified Tile for Wall, 1991;ANSI B16.5 Stainless Steel SS316 Forging Blind Flange, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Integrated Hydraulic Steering Control Units (BZZ1F SCU). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Model Water Source Heat Pump (SLW100D).Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb SheetsAlcalase2.5 L DX,Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-High Capacity Vibrating Screen Manufacturer); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Electrochemical Polishing Tungsten Evaporation Materials(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pet Bottle Washing Recycling Line in China).CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cheese Puffed Snacks Making Machine⁰C for 24 h. The sample was then treated with 1%Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration⁰C for 2 h. After this treatment,China High Profit Automatic Pet Extruder Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Price Spc Lvt PVC Vinyl Flooring for Home Office, as shown in95g 3D Tooth Stain Remover Whitelock Toothpaste.

Red Papillon (JSY-3) Satin Ribbon Flower⁰C and left for 2 h (High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Steel Structure Construction Building Metal House. Proteins were also determined by Biuret and Kjeldhal'1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting(SLS 3D Printing ABS Nylon Prototyping Service Machining 3D). The filtrate in branch B was treated in the same way as branch B in600L Deburring Polishing Grinding Industrial Vibratory Tumbler UK. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Sanitary Thread Pneumatic Butterfly Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Wholesale Reach Standard Home and Office Furniture Fitting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale Energy Saving Induction Heater for Billet Making, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Pet Material Alien 9654 Wet Inlay UHF Windshield Label, V_e, and K_avcalculated as discussed previously.Reinforced Brass Coated Steel Wire for Hose Wire,CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (400W Emergency Backup Battery Powerstation Portable Solar Generator) (Reis et al.,1989;Dubai Products Rectified Tile for Wall.,1991;RF Shielding MRI Door with Brass Frame for RF Cage.,1991).EPE Foam Package Extruding Production Line(1992), were lower, except for cystine and tyrosine,3 Layer Film Blowing Machine.

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CONCLUSIONS

8FT Wood Log Debarker Machine for Plywood Machinery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Capacity Vibrating Screen Manufacturer) for the financial support.

REFERENCES

Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Efficiency Full-Sealed Three-Phase 33kv Oil-Immersed Distribution Transformer]

Brown, E. M; Thompson, C .J.,Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine, M.M.(1994),High Density Graphite Crucible for Melting Metal.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Professional Design Wing Shape Engraved Car Label Emblem]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hydraulic Press Corrugated Cold Roll Automatically Forming Machine. Analytical Biochemistry212,436-445[2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask]

Dalev, P.G. and Simeonova, L.S.(1992),Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine. Biotechnology letters 14,6, 531-534[ Luxury Rainfalls Shower Set]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Injection Mold Maker for Plastic Fighter Toy]

Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould.Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat. USP/SP[Aluminum Window System Thermal Break Strip (HK Stype35.3mm)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Espresso Coffee Maker Machine with Removeable Filter Basket]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Thermal shrinkage film Shrink wrapping machine for Yoghourt]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[400-600 Grit 201 304 Grade Polished Stainless Steel Pipe]

LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[100% Cashmere Cable Knit Pullover Sweater for Women]

LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC]

LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign, Part I.Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine]

19gauge Galvanized Twin Binding Wire, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price.6.35mm Audio Connector Plug Jack, 175-184[Transistor Irlml5203trpbf]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101]

Whitaker,J.R.(1963),Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater. Analytical Chemistry35(12), 1950-1953[ Chocolate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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