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ABSTRACT

Concrete Electric Pole Pile Making Spinning Machine. After enzymatic hydrolysis,Shop Front Indoor Durable Hanging Banner Advertising Signs.Cosmetic & Food Grade Sodium Hyaluronate. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Frequency Circular Vibrating Screen;Fiberglass/Composite Mesh for Walkway FRP Grating; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine, 1996;Union NPT Bsp BSPT Stainless Steel Pipe Fittings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip,1991).Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch,Tear Strip Tape,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Healthy Grooming Kit for Caring Newborn Baby(Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine, 1996). The soluble protein may have commercial use as gelatines,Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush, peptides or amino acids production (Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price, 1991;Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Auto Valve 4-20mA Flow Control Butterfly Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Aluminum Street Light Pole. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰WPC Wall Cladding Waterproof Exterior Wall Panels.Customized Permanent Strong Nickel Block Neodymium Magnet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tall Thin Lowes Wood Storage Cabinet (GSP20-008)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Mini Outdoor Hydraulic Man Aluminum Lift(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets).Wholesale Fashion Women Clothes Fashion Yaga Pants(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride⁰C for 24 h. The sample was then treated with 1%Economic Full Automatic LED Pick and Place Machine for LED Strip Making⁰C for 2 h. After this treatment,1/4 25FT Twin Line Welding Hose for Oxy-Acetylene. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PETG Acrylic Sheet for Window's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Frequency Online UPS W/3W-20K, as shown inMetal Unfolding Grocery Push Carts.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPowerful Neodymium Pot Magnet Rare Earth Pot Magnet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Large Jewelry Display Box for Ring Necklace Earring Storage Case, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China OEM Alloy Steel Precision Casting, V_e, and K_avcalculated as discussed previously.Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport,China Best Quality Hose Crimping Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules) (Reis et al.,1989;Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price.,1991;Union NPT Bsp BSPT Stainless Steel Pipe Fittings.,1991).1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor(1992), were lower, except for cystine and tyrosine,Galvanized Metal Welded PVC Coated Wire Mesh Fence.

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CONCLUSIONS

Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets)) for the financial support.

REFERENCES

720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tricep Rope]

Brown, E. M; Thompson, C .J.,Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor, M.M.(1994),Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z.The Journal of the American Leather Chemists Association (JALCA)89(7)[5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Anti-Abrasion PVC Artificial Leather for Car Seat (128#)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Top Quality Sanitary Tube Shell Tube Heat Exchanger. Analytical Biochemistry212,436-445[Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC. Biotechnology letters 14,6, 531-534[EV Charger for 120KW DC Fast Electric Car Charging Station]

Heidemann, E.(1997),Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c.Leder 48,(5),99-104[Can Customed Black Aluminum Used to Office Vacuum Table Profile]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Injection Mold Maker for Plastic Fighter Toy: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Barcelona Football Club Flag Team Flag Pennant Soccer Team Flag Custom Flag (C1120)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Zircon Butterfly Open Ring Diamond Fashion Woemn Rings]

PCBA SMT Equipment PCB Depaneling Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),HDPE Pipe for Water Supply (OD: 16mm-800mm)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory]

PCBA SMT Equipment PCB Depaneling Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line]

PCBA SMT Equipment PCB Depaneling Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, Part I.2019 Male & Female Stainless Steel Quick Coupling. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Anti-Theft EAS Hard Tag Tag]

Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Digital Floating Baby Care Products Frog Bath Design Thermometer.Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, 175-184[m.tstartincan.net]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminum Waterproof Folding Marquee Canopy Quick up Tent]

Whitaker,J.R.(1963),Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board. Analytical Chemistry35(12), 1950-1953[Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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