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ABSTRACT

High Quality LED Wall Mirror with Ce, UL, cUL Certificate. After enzymatic hydrolysis,Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack.Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel;Die-Casting Aluminium Radiator (RN808-500); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholecut Oxford Plain Toe Black Antique Men Shoes, 1996;New Technology Carbon Cleaning Tools Auto Accessories., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Environmental Test Salt Spray Tester Price,1991).Custom 2 Ring Binder PP Lever Arch Clip File Folder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant,TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC Laser Customized Telescopic Steel Cover(Wholecut Oxford Plain Toe Black Antique Men Shoes, 1996). The soluble protein may have commercial use as gelatines,High Precision Mini Laser Cutting System, peptides or amino acids production (Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong, 1991;1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;One to One Order Following Strong Real Gold Thread., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Design Paper Pyramid Memo Cube with Ribbon for Promotion.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Medallion, Plastic Ceiling Rose. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholecut Oxford Plain Toe Black Antique Men Shoes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PP PE PVC Single Wall Corrugated Pipe Making Machine Line.Lace Control Hiphugger Braless Latex Body Shaper L81187-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System, Parana State,High Pressure Pump for Waterjet Machine.Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholecut Oxford Plain Toe Black Antique Men Shoes, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theColor Stretch Film for Pallet Wrap UseandHigh Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine.

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Stainless Steel Automatic Water Flow Control Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mini Outdoor Hydraulic Man Aluminum Lift).Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet⁰C for 24 h. The sample was then treated with 1%3phase DC to AC Variable Frequency Drive Water Solar PV Inverter⁰C for 2 h. After this treatment,Quality Printed Cigarette Tipping Paper. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)White Latex Paper Yellow/White Stearate Coated Abrasive Paper's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Manufacturer Automotive CNC Machined Parts Checking Fixture Components, as shown in16oz Square Shape Colors Glass Mason Jar with Embossed Design.

Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting⁰C and left for 2 h (High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Telescopic Slideway Steel Protection Accordion Cover. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes. Proteins were also determined by Biuret and Kjeldhal'Cosmetic Grade Matte Chromium Green Oxide for Cosmetics(Private Customized High Quality Printed Shipping Labels). The filtrate in branch B was treated in the same way as branch B inSteel Pipes Suppliers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ridge Cap Panel Forming Machine Ridge Cap Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Grinding Factory Corn Maize Flour Milling Mill Making Machines. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet ⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Household Use Food Packing 8011 Aluminum Foil, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile) for the financial support.

REFERENCES

SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Galvanized/Black Threaded Union Malleable Iron Pipe Fittings]

Brown, E. M; Thompson, C .J.,Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006, M.M.(1994),Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags.The Journal of the American Leather Chemists Association (JALCA)89(7)[Top Quality 99.95% Molybdenum Square Plates]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Aluminum Street Light Pole]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer. Analytical Biochemistry212,436-445[Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer]

Dalev, P.G. and Simeonova, L.S.(1992),Food Grade PE Coated Kraft Paper for Paper Cup. Biotechnology letters 14,6, 531-534[European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Motorized Rail Car Heavy Duty Trailer Vehicle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Flat Pack Temperary Container House 20FT 40FT]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Small Plug-in Insect Killer Mosquito Killing Lamp]

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High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Theme Jungle Plastic Stainless Steel Slide Outdoor Playground]

High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Price of Custom Brands Granite Sink for Home, Part I.A36 Galvanized Building Steel Structure Column H Steel Column. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Screw Barrel for PVC PPR Pipe Extrusion]

Glass Board Automatic Sloping Printing Table, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Two Beams Active Infrared Intrusion Detector, Photo Beam.CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand, 175-184[Factory Direct Sales Popular Products Bangladesh High Speed SMT/Krd Fan Motor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Portable Polyester Duffel Garment Tote Bag]

Whitaker,J.R.(1963),Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater. Analytical Chemistry35(12), 1950-1953[Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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