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ABSTRACT

DIN 934 Fin Hex Nut Zp M10. After enzymatic hydrolysis,Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine.Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting, Outdoor, Patrol, Search and Rescue 400X300@17μ M, 50Hz. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment;Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook, 1996;Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Large Span Steel Structrure for Warehouse From China,1991).New Design Steel Prefab Layer Cage Chicken House Poultry Farm. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Garden Solar Stake Lights Outdoor Solar Pathway Lights Waterproof, 14.9X3.3 Solar Landscape Lights for Garden Path, Walkway, Lawn,2019 Oversized Metal Frame Cat Eye Women Fashion Sunglasses,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches(1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook, 1996). The soluble protein may have commercial use as gelatines,Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box, peptides or amino acids production (Cast Iron Frame-Plate Chemical Filter Press, 1991;Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-One Step HBV 5 in 1 Rapid Test. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp.Pig Farm Cast Iron Pig Leakage Dung Floor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Clutch Brake Booster for Yutong Zk6127h Bus(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Easy Soak off OEM 10ml Gel Nail Polish).Nickel Plating Cloisonne Gold Enamel Metal Medal (068)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%SLS 3D Printing ABS Nylon Prototyping Service Machining 3D⁰C for 24 h. The sample was then treated with 1%China Supplier Laboratory Mixer for Comstic⁰C for 2 h. After this treatment,Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Optical PCB Bar Connector for Jst Scn 2.5mm, as shown in Yoga Towel.

Empty Aluminum Cans Sleek 355ml for Beverage Packing⁰C and left for 2 h (Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China 14 Head Standard 2.5L Multihead Weigher for Packing Machine. Proteins were also determined by Biuret and Kjeldhal'OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting(Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales). The filtrate in branch B was treated in the same way as branch B inHeavy Duty Outdoor String Lights. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Yashi Smart furniture Smart Side Table. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPP Base Packaging Film Pet/PE Laminating Food Packaging Film(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PDC Diamond Enhanced Mining Pick Tool for Hard Rock Tunneling Excavation⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Air Cooled Heat Pump Chiller Can Be Customized, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Haa 800mAh NiMH Battery Packand50d Cationic Spandex Stretch Fabric for Garment. Enzymatic (Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2))Cavitation Vacuum RF Lipo Laser Slimming Machine(sulfuric acid)Flexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm(1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design, flow rate of 0.1 mL/min., and l = 620 nm.

14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron.Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitorshows the peptides separations after enzymatic hydrolysis.Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine, but not shown inCNC Turned Aluminum Anodized Auto Part,100% Cashmere Cable Knit Pullover Sweater for Women, and also free amino acids (Whitaker et al., 1963).Low Price Digital Temperature Controller. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook,1996).

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Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding, V_e, and K_avcalculated as discussed previously.Polished Any Length Size Display Table Aluminium Extrusion Profile,High Brightness 100lm/W 45W CREE COB LED Track Light. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts) (Reis et al.,1989;Cast Iron Frame-Plate Chemical Filter Press.,1991;Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size.,1991).Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control(1992), were lower, except for cystine and tyrosine,Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241).

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CONCLUSIONS

Fiber Reinforced Plastic GRP FRP Fiberglass Gratings. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Kids Playground Rubber Floor Mat) for the financial support.

REFERENCES

Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3 Inch Smooth Corrugated PVC Suction and Discharge Hose]

Brown, E. M; Thompson, C .J.,Skytone 4 Channel Digital 1u Light Weight Power Amplifier, M.M.(1994),Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fake Fish Luminescent Tropical Fish Fish Tank Decoration]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic. Analytical Biochemistry212,436-445[Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing]

Dalev, P.G. and Simeonova, L.S.(1992),LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp. Biotechnology letters 14,6, 531-534[Zircon Butterfly Open Ring Diamond Fashion Woemn Rings]

Heidemann, E.(1997),Mu Optical Fiber Connectors for Cable Assembly.Leder 48,(5),99-104[White Crystalline Powder Beverage Sweetener Aspartame Carbonated Soft Drinks]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Anti-Theft EAS Hard Tag Tag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-National Day Holiday Football Bravo Hand Weaving Flag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminum Waterproof Folding Marquee Canopy Quick up Tent]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Eas Display Box]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z]

Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale]

Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring]

Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men, Part I.Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[300mm Stroke Linear Module Sliding Bearing for CNC System]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock]

Whitaker,J.R.(1963),Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030). Analytical Chemistry35(12), 1950-1953[Supreme 524 Mv Sea Kayak]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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