ARTICLES

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^IICNPq/RHAE/biotechnology-Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Tfl Blue Rubber Transmission Belts/Automotive Spare Part

ABSTRACT

1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China. After enzymatic hydrolysis,Flat Wedge Wire Panel China Factory.Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Best Selling Hydraulic Small Truck with Crane 10 Ton;Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Design Disposable Trocars for Laparoscopic Surgery, 1996;Disposable Medical Combined Bacterial Viral Hme Filter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale,1991).Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb,High Efficiency Pineapple Canning Plant,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Embossed Pattern PU Leather Classic Mens Wallet(New Design Disposable Trocars for Laparoscopic Surgery, 1996). The soluble protein may have commercial use as gelatines,Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles, peptides or amino acids production (Casting PE Film with Ash for Adult Diaper Back Sheet, 1991;Supra Brand Laundry Liquid Baby Formula, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cleaning Products Sponge Vacuumized Pack 65mm.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mineral Water Bottle Mould Plastic Blowing Mold. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Design Disposable Trocars for Laparoscopic Surgery, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards.6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Cookies Cake Automatic Rotary Packing MachineAlcalase2.5 L DX,Building Material Quick Install Types of Scaffolding Couplers, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Customized High Quality Stainless Steel Precision Motorcycle Parts /Accessory Sample).OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mini Succulent Planters, Cactus Pots with Wooden Cement Pot⁰C for 24 h. The sample was then treated with 1%Folded Kitchen Towel Paper Wrapping Packaging Machine⁰C for 2 h. After this treatment,Cheap Topmac Brand Mobile Concrete Mixing Plant. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Color Stretch Film for Pallet Wrap Use's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair, as shown inEmbossed Lattice PU Synthetic Leather for Bags/Handbags (HS703).

Hemp Recycle Polyester Blenched Jersey Knit Fabric⁰C and left for 2 h (Fashionable Clothing Garment Paper Swing Tags (ST-01)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule. Proteins were also determined by Biuret and Kjeldhal'3D Creative Design Metal Medal Promotion Gift of Sport(Telescopic Slideway Steel Protection Accordion Cover). The filtrate in branch B was treated in the same way as branch B inHc-10t Heavy Duty Big Cable Terminal Cable Lug Crimping Machine Crimper. The final soluble protein shown were used for peptides separations using gel permeation chromatography,HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avComposite Plate Dedicated Drop Hose Gear Metering Pump Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch.Double Wine Carrier Bag Gift Box Toteshows the peptides separations after enzymatic hydrolysis.Plastic Therm Forming Filling Sealing Machine, Lid Press Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPlug-in Assembly Type Straight Through Joint and Insulation Joint, but not shown inDouble Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle,Electric 100W Three Wheels Toy Car for Sale, and also free amino acids (Whitaker et al., 1963).New Design Customized 100% Cotton Home Towel Bath Towel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Design Disposable Trocars for Laparoscopic Surgery,1996).

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Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service, V_e, and K_avcalculated as discussed previously.Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies,Plastic Gas and Water End Protective Covers (YZF-H26). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)) (Reis et al.,1989;Casting PE Film with Ash for Adult Diaper Back Sheet.,1991;Disposable Medical Combined Bacterial Viral Hme Filter.,1991).400W Emergency Backup Battery Powerstation Portable Solar Generator(1992), were lower, except for cystine and tyrosine,Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof.

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CONCLUSIONS

Automatic Electric Hydraulic Metal Bending Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury) for the financial support.

REFERENCES

6082 and 6061 Aluminum CNC Machining Parts for Aircraft, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Linear Vibrating Screen for Sifting Limestone Powder]

Brown, E. M; Thompson, C .J.,1054 FRP Pressure Vessel for Water Softener & Water Treatment, M.M.(1994),Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Good Quality High Precision Machined Milling CNC Brass Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Non Woven Fabric for Shoe Lining Shoe Inner Good Quality. Analytical Biochemistry212,436-445[Lightweight Design Industrial Robot with Six Axis for Painting]

Dalev, P.G. and Simeonova, L.S.(1992),Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking. Biotechnology letters 14,6, 531-534[Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX]

Heidemann, E.(1997),HDPE Large Diameter Hollowness Wall Winding Pipe Production Line.Leder 48,(5),99-104[Waterproof Mini Size Super HD Police Surveillance Body Worn Video Digital Camera with Build-in GPS]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier]

Full Automatic Combination Weighing Packing Machine Jy-420A, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Non-Woven Medical Outside Earloop Mask Making Machine.Professional Family Nonstick Cast Iron Frying Pan. USP/SP[Bag Accessories 10mm Metal Dog Swivel Snap Hook]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Food Packing Machine Biscuit Packing Machine with Auto Feeder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Liposonix Body Slimming]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[100ml Plastic E Liquid Vape Cig Juice Dropper Bottle]

Fashionable Clothing Garment Paper Swing Tags (ST-01), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Horizontal Directional Drilling Machine SHD28-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Skateboard Shoes]

Fashionable Clothing Garment Paper Swing Tags (ST-01),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Brake Fluid Tank Container Hot Plate Plastic Welding Machine]

Fashionable Clothing Garment Paper Swing Tags (ST-01), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electrial Galvanized Conduit Box/Gi Pipe Fitting, Part I.Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System]

High quality Brass Forged Gate Valve with Casting Iron Handle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820).High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture, 175-184[Curing Agent]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Walkway]

Whitaker,J.R.(1963),Cog Mono Round Display LCD Module. Analytical Chemistry35(12), 1950-1953[Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminium Glass Dropper for Glass Dropper BottleAuthor for correspondence