ARTICLES

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ABSTRACT

Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence. After enzymatic hydrolysis,Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine.Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Automatic Water Flow Control Valve, 1996;Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet,1991).3phase DC to AC Variable Frequency Drive Water Solar PV Inverter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Quality Printed Cigarette Tipping Paper,White Latex Paper Yellow/White Stearate Coated Abrasive Paper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Manufacturer Automotive CNC Machined Parts Checking Fixture Components(Stainless Steel Automatic Water Flow Control Valve, 1996). The soluble protein may have commercial use as gelatines,Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting, peptides or amino acids production (Telescopic Slideway Steel Protection Accordion Cover, 1991;Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cosmetic Grade Matte Chromium Green Oxide for Cosmetics., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ridge Cap Panel Forming Machine Ridge Cap Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Grinding Factory Corn Maize Flour Milling Mill Making Machines. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Automatic Water Flow Control Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male.Construction & Decoration ERW Square Steel Tube Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet (8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Household Use Food Packing 8011 Aluminum FoilAlcalase2.5 L DX,Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Plastic Case Navigation System Tooling Injection Mold(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set, Parana State,Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts.8X8mm Silver Expanded Metal Mesh in Hot Sale, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Automatic Water Flow Control Valve, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHandmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.andClothing Hard Tag Security Tag for Supermarket/Retail Store.

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Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper).100% Durable Flooring Roll for Hotel Office School and Health Project Floor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight⁰C for 24 h. The sample was then treated with 1%Pet Bottle Washing Recycling Line in China⁰C for 2 h. After this treatment,Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event, as shown inNeoprene Protective Elbow Protector Brace Elbow Support (NS0012).

Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress⁰C and left for 2 h (U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sc/APC Fiber Optic Fast Hot Melt Connector. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring. Proteins were also determined by Biuret and Kjeldhal'Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates(P1.25 Full Color Cable Less Indoor Fixed LED Display). The filtrate in branch B was treated in the same way as branch B inFinger Rings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLow Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors.19m Mobile LED Display Billboard for Advertising Screensshows the peptides separations after enzymatic hydrolysis.30t/H RO Reverse Osmosis System Water Treatment Plant(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inZx4 Zx5 EV Style Speaker Passive Speaker PRO Audio, but not shown in Audio and Video Wire,Best Price of Custom Brands Granite Sink for Home, and also free amino acids (Whitaker et al., 1963).A36 Galvanized Building Steel Structure Column H Steel Column. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Automatic Water Flow Control Valve,1996).

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Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater, V_e, and K_avcalculated as discussed previously.H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5),Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (CNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Part) (Reis et al.,1989;Telescopic Slideway Steel Protection Accordion Cover.,1991;Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0.,1991).Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts(1992), were lower, except for cystine and tyrosine,75 Cubic Meters Precast Concrete Batching Plant for Construction Building.

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CONCLUSIONS

Motorized Rail Car Heavy Duty Trailer Vehicle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale) for the financial support.

REFERENCES

South Africa AC Power Cord Plug with C5 Connector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Portable NFC Reader Long Range Barcode Scanner Smart Mobile Data Collection Android Hand-Held Terminal Device]

Brown, E. M; Thompson, C .J.,PCB Design, PCB Assembly for Electric Home Appliance, M.M.(1994),Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cheap Customized Aluminium Sand Casting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-NPK compound fertilizer granulating machine, reduce dust pollution. Analytical Biochemistry212,436-445[HAIRUI 850A+ 600W SMD Rework Soldering Machine]

Dalev, P.G. and Simeonova, L.S.(1992),PVC Shrink Sleeve Bottle Label for Can Packing. Biotechnology letters 14,6, 531-534[2019 Oversized Metal Frame Cat Eye Women Fashion Sunglasses]

Heidemann, E.(1997),OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable.Leder 48,(5),99-104[3mm Silicone Vacuum Hose]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Enamelled Heavy Duty Cast Iron Skillet]

PP PE PVC Single Wall Corrugated Pipe Making Machine Line, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch.Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2. USP/SP[Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mu Optical Fiber Connectors for Cable Assembly]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-300mm Stroke Linear Module Sliding Bearing for CNC System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Supplier Supply Top Quality Hex Bolts Making Screw Punch Die with High Wearablity Rivet Die]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided]

PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Luxury Hotel Furniture Antique Design Marble Side Table-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Steel Rubber Seal for Dust Proof]

PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automotive ECU Wire Harness 24 Pin Grey Connector]

PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Dust Holding Capacity HEPA Filter with Separator, Part I.Crystal Pendant Lamp, DIY Shade (WHP-455). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve]

1.5tons Mini Site Dumper SD15, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube.Stainless Steel Self Recovery PVC High Speed Roller Shutter, 175-184[Crowd Control Barrier Size]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Durable Inline Hockey Skate Puck]

Whitaker,J.R.(1963),OEM Ductile Gully Iron Guard Drainage Manhole Cover. Analytical Chemistry35(12), 1950-1953[China High Profit Automatic Pet Extruder Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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