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ABSTRACT

22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine. After enzymatic hydrolysis,Supermarket Combined Island Chest Freezer Commercial Refrigerator.PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Black and White Hard Laptop Case Travel Suitcase, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Concrete Road Crack-Pouring Machine for Construction, 1996;Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China OEM Alloy Steel Precision Casting,1991).Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Best Quality Hose Crimping Machine,1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Galvanized Metal Welded PVC Coated Wire Mesh Fence(Concrete Road Crack-Pouring Machine for Construction, 1996). The soluble protein may have commercial use as gelatines,Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand, peptides or amino acids production (720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera, 1991;Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Top Quality Sanitary Tube Shell Tube Heat Exchanger.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Concrete Road Crack-Pouring Machine for Construction, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c.High Precision iPhone Cases Fiber Laser Marking Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Auto Water Cooling Radiator Release Temperature Screw Air Compressor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Digital Floating Baby Care Products Frog Bath Design Thermometer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1850mm*80mm Stable Power Laser Tube).Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board⁰C for 24 h. The sample was then treated with 1%FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters⁰C for 2 h. After this treatment,Colorful Asphalt Shingle for Roof Water Proof. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Durable Inline Hockey Skate Puck's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane), as shown inHigh End L Shape Modular Sectional Sofa Patio Furniture Set Garden.

18mm Thickness Best Quality Poplar Birch Commercial Plywood⁰C and left for 2 h (Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box. Proteins were also determined by Biuret and Kjeldhal'Nylon Hammer Fixings Drive Anchor(Diamond Cutting Blades for Asphalt). The filtrate in branch B was treated in the same way as branch B inCool Shade Cool Tube Air Cooled Reflector. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Electrolyte Effervescent Tablet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/304 321 310 310S Welded Decorative Stainless Steel Pipe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part.High Quality 3000L Composting Machine with Lower Priceshows the peptides separations after enzymatic hydrolysis.Aluminum Plate Fin Heat Exchanger/Oil Cooler Core(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inExtendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10), but not shown inGlow in Dark Ceramic Sublimation Mug 11oz,3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection, and also free amino acids (Whitaker et al., 1963).3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Concrete Road Crack-Pouring Machine for Construction,1996).

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CONCLUSIONS

Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (HDPE Pipe for Water Supply (OD: 16mm-800mm)) for the financial support.

REFERENCES

High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Top Water Spraying Fresh Fruits Vegetable Washing Machine Price]

Brown, E. M; Thompson, C .J.,Hydraulic Hose SAE 100 R1AT, M.M.(1994),Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine.The Journal of the American Leather Chemists Association (JALCA)89(7)[CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07. Analytical Biochemistry212,436-445[Reusable Silicone Food Storage Bag]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes. Biotechnology letters 14,6, 531-534[1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting]

Heidemann, E.(1997),Ts-Micro Portable Defense Loudspeaker System.Leder 48,(5),99-104[China Factory Mayflay Machinery Q37 Series Hook Type Shot Blasting Machine for Metal Rust Cleaning with Ce]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment]

Glow in Dark Ceramic Sublimation Mug 11oz, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm.Medical Supply for Laparoscopic Instrument (MIM Straight Scissors). USP/SP[ Vodka]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic Nylon Sheet with High Temperature]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-86bl3a125 1.65A 660W 310V BLDC: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Curling and Shine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Lumen Outdoor IP65 100W LED Flood Light]

Tamper Evident Branded Printed Packing Pet Adhesive Security Tape, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector]

Tamper Evident Branded Printed Packing Pet Adhesive Security Tape,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould]

Tamper Evident Branded Printed Packing Pet Adhesive Security Tape, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hotel Use and Steel Material Poultry House for 10000 Chickens, Part I.50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Price Spc Lvt PVC Vinyl Flooring for Home Office]

Kontron One-Piece 10-Lead EKG Cable with Resisitance, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Best Price of Peanut Digger / Groundnut Harvester in Australian Africa.China Manufacture Table Type CNC Horizontal Boring Milling Machine, 175-184[250V 16A 10-20m Schuko GS Socket Cable Reel Extension Socket]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China]

Whitaker,J.R.(1963),White Electrical Fireplace Warmer. Analytical Chemistry35(12), 1950-1953[Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

99% Al2O3 High Alumina Ceramic BallAuthor for correspondence