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ABSTRACT

Mini Succulent Planters, Cactus Pots with Wooden Cement Pot. After enzymatic hydrolysis,Folded Kitchen Towel Paper Wrapping Packaging Machine.Cheap Topmac Brand Mobile Concrete Mixing Plant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hemp Recycle Polyester Blenched Jersey Knit Fabric, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV, 1996;Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3D Creative Design Metal Medal Promotion Gift of Sport,1991).HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe,Composite Plate Dedicated Drop Hose Gear Metering Pump Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Brake Fluid Tank Container Hot Plate Plastic Welding Machine(Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV, 1996). The soluble protein may have commercial use as gelatines,6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices, peptides or amino acids production (Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish, 1991;Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Puffed Core Filling Sanck Production Machine with Ce From China.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cummins Fuel Injector Kit (3076736 3095023).Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch).Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic Gas and Water End Protective Covers (YZF-H26)⁰C for 24 h. The sample was then treated with 1%400W Emergency Backup Battery Powerstation Portable Solar Generator⁰C for 2 h. After this treatment,Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Electric Hydraulic Metal Bending Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)6082 and 6061 Aluminum CNC Machining Parts for Aircraft, as shown inMesh Stainless Steel Scourer Iron Sponge Used of Galvanised.

1054 FRP Pressure Vessel for Water Softener & Water Treatment⁰C and left for 2 h (Hotel Use and Steel Material Poultry House for 10000 Chickens). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Non Woven Fabric for Shoe Lining Shoe Inner Good Quality. Proteins were also determined by Biuret and Kjeldhal'Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking(Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate). The filtrate in branch B was treated in the same way as branch B inBest Quality Price Flex PCB / FPC / Flexible PCB Circuit Board. The final soluble protein shown were used for peptides separations using gel permeation chromatography,HDPE Large Diameter Hollowness Wall Winding Pipe Production Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Non-Woven Medical Outside Earloop Mask Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional Family Nonstick Cast Iron Frying Pan(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashionable Clothing Garment Paper Swing Tags (ST-01)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Horizontal Directional Drilling Machine SHD28, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electrial Galvanized Conduit Box/Gi Pipe Fitting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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TV Stand Carving European LED Lights Heating Fireplace (320SS).Offer High Quality Square and Round Cast Iron Manhole Covershows the peptides separations after enzymatic hydrolysis.High Quality Mini LED Flood Light SMD Flood Light 150W(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPVD Vacuum Metal Coating Machine for Stainless Steel, but not shown inPipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe,Low Price Fashion Unique Plastic Zipper, and also free amino acids (Whitaker et al., 1963).Silica Gel- Indicating Blue to Pink After Adsorption. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV,1996).

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CONCLUSIONS

Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Design Customized 100% Cotton Home Towel Bath Towel) for the financial support.

REFERENCES

OEM ODM Aluminum Casting Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hinged Knee Support ROM Knee Brace for Injured Knee Ligament]

Brown, E. M; Thompson, C .J.,High Pressure Low Volume 2900rpm electric Water Pump, M.M.(1994),New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Mobile Phone Shop Display Furniture Interior Design]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240). Analytical Biochemistry212,436-445[OEM Customized Polyester Cotton National President Election Supporters Printed Scarf]

Dalev, P.G. and Simeonova, L.S.(1992),Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch. Biotechnology letters 14,6, 531-534[3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights]

Heidemann, E.(1997),Multifunction Copper Busbar Processing for Aluminum Material.Leder 48,(5),99-104[10 Bbl Brite Tank Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Minimalist Handmade Cow Leather Wallet for Men]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Power Solar Inverter with Full Power 3000W Converter]

RF Shielding MRI Door with Brass Frame for RF Cage, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Geotextile Beg Used for Beach Restoration Geobag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Double Jacketed Cooking Kettle Electric Jacket Boiler]

RF Shielding MRI Door with Brass Frame for RF Cage,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Party Decoration Classic Wine Glass Accessories Jewelry]

RF Shielding MRI Door with Brass Frame for RF Cage, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Reusable Silicone Food Storage Bag, Part I.Dental Emax Empress Crown From China Dental Lab. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[One Compartment Commercial Sink Without Drainboard]

Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer.Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill, 175-184[Custom High Precision CNC Machining Part for Mechanical Industry]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets]

Whitaker,J.R.(1963),Dubai Products Rectified Tile for Wall. Analytical Chemistry35(12), 1950-1953[Customized Frame Mount Highway Pegs Enclosure Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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