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ABSTRACT

Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish. After enzymatic hydrolysis,Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding.High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cummins Fuel Injector Kit (3076736 3095023), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5, 1996;Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Therm Forming Filling Sealing Machine, Lid Press Machine,1991).Electric 100W Three Wheels Toy Car for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Design Customized 100% Cotton Home Towel Bath Towel,Toughened Railing Glass,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Delrin Sheets with Good Wear Resistance(Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5, 1996). The soluble protein may have commercial use as gelatines,High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery, peptides or amino acids production (Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service, 1991;Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Gas and Water End Protective Covers (YZF-H26)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.400W Emergency Backup Battery Powerstation Portable Solar Generator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Electric Hydraulic Metal Bending Machine.6082 and 6061 Aluminum CNC Machining Parts for Aircraft-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1054 FRP Pressure Vessel for Water Softener & Water Treatment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas SystemAlcalase2.5 L DX,Non Woven Fabric for Shoe Lining Shoe Inner Good Quality, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)HDPE Large Diameter Hollowness Wall Winding Pipe Production Line(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Silica Gel- Indicating Blue to Pink After Adsorption. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOffer High Quality Square and Round Cast Iron Manhole Cover(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Road Safety Reflective Waterproof Plastic Reflectors, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking) for the financial support.

REFERENCES

Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High-End Intelligent Automatic Car Washer]

Brown, E. M; Thompson, C .J.,Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill, M.M.(1994),Dubai Products Rectified Tile for Wall.The Journal of the American Leather Chemists Association (JALCA)89(7)[Healthy Grooming Kit for Caring Newborn Baby]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Composite Machining Phenolic Titanium Parts Products Supplier]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ANSI B16.5 Stainless Steel SS316 Forging Blind Flange. Analytical Biochemistry212,436-445[2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)]

Dalev, P.G. and Simeonova, L.S.(1992),3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection. Biotechnology letters 14,6, 531-534[High Frequency Online UPS W/3W-20K]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ 750mm Cuttable Capacitive Diesel Fuel Level Sensor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hw801 140*38mm Customized Promotion PVC Bookmark Reading Ruler Magnifier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[100% Polyester Luxury Bedding Comforter Fleece Blanket Throw]

Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Different Wood Options Rigid Spc Core Vinyl Flooring]

Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Quality OEM Stainless Steel Casting Rings]

Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325, Part I.High Capacity Vibrating Screen Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Indoor Tub Customized Shower/Free Standing Shower Enclosure]

Electrochemical Polishing Tungsten Evaporation Materials, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes.White UTP Cat5e UK Type Tooless RJ45 Keystone Jack, 175-184[Tents Lights]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer]

Whitaker,J.R.(1963),High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill. Analytical Chemistry35(12), 1950-1953[Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Manufacturer Multi Span Polycarbonate Industrial Green HouseAuthor for correspondence