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ABSTRACT

New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain. After enzymatic hydrolysis,New 14 Inch Diamond Saw Blade for Granite Cutting.TV Antenna (CH50006). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tongkai Classic Business Hardcover Leather Notebook, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customization! Bdy380CD Horizontal Directional Drilling Machine, 1996;500kg Capacity 14m Height Semi Electric Scissor Lift., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale,1991).DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose,Small Batch Processing Football Net,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router(Customization! Bdy380CD Horizontal Directional Drilling Machine, 1996). The soluble protein may have commercial use as gelatines,New Condition Textured Peas Soy Protein Making Machine, peptides or amino acids production (Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556, 1991;5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;SBR Round DOT Horse Stall Mat, Rubber Stable Mat., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Frame Mount Highway Pegs Enclosure Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Double Wine Carrier Bag Gift Box Tote. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customization! Bdy380CD Horizontal Directional Drilling Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket.40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:RF Coaxail BNC Male to BNC Male Plug Connector Adapter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector).Extruder Floating/Sinking Fish Feed Pellet Dryer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Durable Silicone Rubber Seal Ring for Pressure Cooker⁰C for 24 h. The sample was then treated with 1%High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies⁰C for 2 h. After this treatment,Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Quality Assured Magnetic Building Blocks for Children's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System, as shown inRapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould.

Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary⁰C and left for 2 h (Best Price Spc Lvt PVC Vinyl Flooring for Home Office). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint. Proteins were also determined by Biuret and Kjeldhal'Cummins L10 Engine Piston Cooling Nozzle 3070218(1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China). The filtrate in branch B was treated in the same way as branch B inDaisen Brand Recarburizer Carburant Carbon Raiser. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Price Long Life Portable Peltier Air Cooler⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hand Tools 9PCS Hex Key Set CRV Allen Wrench, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Agriculture Indoor Vegetables and Fruits Planting Greenhouse.99% Al2O3 High Alumina Ceramic Ballshows the peptides separations after enzymatic hydrolysis.Night Vision Wide HD Waterproof Car Side View Camera for Bus(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDeluxe Saddle Doctor Chair Dental Stool with Adjustable Seat, but not shown inM16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6,Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade, and also free amino acids (Whitaker et al., 1963).5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Customization! Bdy380CD Horizontal Directional Drilling Machine,1996).

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CONCLUSIONS

Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Glazed Metal Roof Tile Roll Forming Machine with Ce) for the financial support.

REFERENCES

Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Table Cutlery Holder]

Brown, E. M; Thompson, C .J.,Zircon Butterfly Open Ring Diamond Fashion Woemn Rings, M.M.(1994),EVA Puzzle Mat with Letters for Kids Playing Customized Pictures.The Journal of the American Leather Chemists Association (JALCA)89(7)[Embossed Pattern PU Leather Classic Mens Wallet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Gold Parquet Marble Copper Side End Table. Analytical Biochemistry212,436-445[High Quality Custom Golden Colored Powder Pill Organizer]

Dalev, P.G. and Simeonova, L.S.(1992),Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing. Biotechnology letters 14,6, 531-534[Auto/Manual Spray Painting Booth/Cabinet with Best Price]

Heidemann, E.(1997),Foldable 80W Folding Solar Panel for Caravan in Holiday.Leder 48,(5),99-104[Instant Beverage Powder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Irregular Shape Hologram Label Printing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1/4 25FT Twin Line Welding Hose for Oxy-Acetylene]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fashionable and Simple Personality Retro and Exaggerated Earrings Circle Crystal Earrings Earrings Pendant]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Medical Grade Ventilator for Sleep Apnea Breathing Machine]

Oxalic Acid for 99.6% Purity, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Kids Waterproof Softshell Jacket with Brushed Fleece Inside-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Fashion Ladies Backpack PU Leather Backpack]

Oxalic Acid for 99.6% Purity,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Carbon Dioxide Gas with 50L Seamless Cylinder]

Oxalic Acid for 99.6% Purity, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Turned Aluminum Anodized Auto Part, Part I.4-20mA PT100 Temperature Transmitter (PCT202). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Product 90GSM Sublimation Paper for Economic Printing]

ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Grade Nitrous Oxide Gas / Laughing Gas / N2o.Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor, 175-184[Shenzhen Bakeware Supplier Metal Cake Baking Molds]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons]

Whitaker,J.R.(1963),Easy Soak off OEM 10ml Gel Nail Polish. Analytical Chemistry35(12), 1950-1953[24 Cores MPO/MTP Cassette]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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