ARTICLES

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ABSTRACT

Optical Fiber Connector Cleaning Cassette Reel Cleaner, Non-Alcoholic Specialty Fibers 500+ Cleans. After enzymatic hydrolysis,Characteristic Impedance of 75 Ohms High-Foaming Coaxial Cable Rg59, RG6, Rg11 for Home and Commercial Application, Audio and Video Wire.Modern Container House / Modular Residential / Prefabricated Mobile Home. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket, 1996;Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Speed Mixer for Plastic Extrusion Line,1991).High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,10mm Gold Plated Stainless Steel Balls G100 Polish Ball,Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags(Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket, 1996). The soluble protein may have commercial use as gelatines,304 Seamless Stainless Steel Tube, peptides or amino acids production (Stainless Steel Manual 3-Way Plug Valve, 1991;Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Natural Clean Non- Ornamentate Decoration Material Wallpaper.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Super Soft 100% Viscose From Bamboo Bedding Set.Theme Jungle Plastic Stainless Steel Slide Outdoor Playground-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Micro Sprinkler for Water Saving Irrigation(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable).Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High End L Shape Modular Sectional Sofa Patio Furniture Set Garden⁰C for 24 h. The sample was then treated with 1%ABS Shell Plastic Injection Molding⁰C for 2 h. After this treatment,Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Manual Glass Lifting Machine for Making Double Glass, as shown inNew Small Plug-in Insect Killer Mosquito Killing Lamp.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSuper Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Viscose Sodium Sulphate Anhydrous by Sateri(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Orange Silica Gel 2-4 mm with High Quality⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI, pH 3.25., mobile phase B: buffer solution of 0.01 mol/aluminium discs for fry pan alloy 1060 1100 1050 3003, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

nellcor non-oximax 7pin infant disposable sensor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Sound System Vt215 Line Array Speaker) for the financial support.

REFERENCES

Power Solar Inverter with Full Power 3000W Converter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Steel Silica Sol Precision Casting]

Brown, E. M; Thompson, C .J.,Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve, M.M.(1994),Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Zoo-Tunes Portable Wireless Mini Character Speakers. Analytical Biochemistry212,436-445[Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts]

Dalev, P.G. and Simeonova, L.S.(1992),CF8m Air Actuated Valve Dn15. Biotechnology letters 14,6, 531-534[CNC Aluminum Laser Milling Machinery]

Heidemann, E.(1997),Rectangular Design Ceramic Small Glass Basin Sink for Bathroom.Leder 48,(5),99-104[Couches Beds Furniture]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Environmental Test Salt Spray Tester Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rectangular Design Ceramic Small Glass Basin Sink for Bathroom]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wheel Bearings]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Oxalic Acid for 99.6% Purity]

Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Liquid Hardener Setting Cement Membrane Curing Concrete-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Tall Thin Lowes Wood Storage Cabinet (GSP20-008)]

Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Duct Evaporative Air Cooler for Warehouse Factory]

Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Silicone Rubber Gasket for Doors Windows, Part I.Top Quality 99.95% Molybdenum Square Plates. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall]

HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Flat Face Stainless Steel Female Jic Quick Release Coupling.Aluminium Glass Dropper for Glass Dropper Bottle, 175-184[Custom Fold up Fan OEM Plastic Hand Fan]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal]

Whitaker,J.R.(1963),L133pbd Common Rail Delphi Nozzle for Ford Mondeo. Analytical Chemistry35(12), 1950-1953[3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Capacity Vibrating Screen ManufacturerAuthor for correspondence