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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Nylon Hammer Fixings Drive Anchor. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,OEM Electrolyte Effervescent Tablet

ABSTRACT

Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh. After enzymatic hydrolysis,6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter.Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant;304 321 310 310S Welded Decorative Stainless Steel Pipe; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Manual Electric Winch Small Winch 220V Winch, 1996;250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar,1991).Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood,Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Plate Fin Heat Exchanger/Oil Cooler Core(Manual Electric Winch Small Winch 220V Winch, 1996). The soluble protein may have commercial use as gelatines,3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection, peptides or amino acids production (3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet, 1991;5-Axis CNC Machining Billet Aluminum Engine Rotary Housing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Clamshell Book Shape Black Cardboard Magnet Gift Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fashionable Simple Printing Mini Key & Coin Purse. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Manual Electric Winch Small Winch 220V Winch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch.Kids Folding Play Mat Kids Play Room Floor Mat-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Roller Window Shutter for Residential Usage(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine, Parana State,OEM Electrolyte Effervescent Tablet.L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Manual Electric Winch Small Winch 220V Winch, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal PartsandTop Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator.

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Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Good Quality High Precision Machined Milling CNC Brass Parts).Ts-Micro Portable Defense Loudspeaker System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm⁰C for 24 h. The sample was then treated with 1%Medical Supply for Laparoscopic Instrument (MIM Straight Scissors)⁰C for 2 h. After this treatment,86bl3a125 1.65A 660W 310V BLDC. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tamper Evident Branded Printed Packing Pet Adhesive Security Tape's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle, as shown inLightweight Design Industrial Robot with Six Axis for Painting.

Hotel Use and Steel Material Poultry House for 10000 Chickens⁰C and left for 2 h (Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Kontron One-Piece 10-Lead EKG Cable with Resisitance. Proteins were also determined by Biuret and Kjeldhal'Best Price of Peanut Digger / Groundnut Harvester in Australian Africa(Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier). The filtrate in branch B was treated in the same way as branch B inFabric Corner Sofa Recliner. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Manufacture Table Type CNC Horizontal Boring Milling Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%White Electrical Fireplace Warmer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLuzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1 Inch Stainless Steel Flexible Exhaust Pipe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car.Food Packing Machine Biscuit Packing Machine with Auto Feedershows the peptides separations after enzymatic hydrolysis.Apex Digital UV Printers for Aluminum Board Printing UV6090(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMulti-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting, but not shown in100ml Plastic E Liquid Vape Cig Juice Dropper Bottle,24 Molds Commercial Ice Cream Cone Maker, and also free amino acids (Whitaker et al., 1963).Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Manual Electric Winch Small Winch 220V Winch,1996).

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CONCLUSIONS

Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel) for the financial support.

REFERENCES

Custom Precision Aluminum Die Casting for Bicycle Accessories, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Air Compressor For Dental]

Brown, E. M; Thompson, C .J.,China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25, M.M.(1994),Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2019 High Quality Memory Mini Portable Wireless Bluetooth Speaker. Analytical Biochemistry212,436-445[Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design]

Dalev, P.G. and Simeonova, L.S.(1992),1200W Three Trio Clustered 810nm Diode Laser for Hair Removal. Biotechnology letters 14,6, 531-534[IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B]

Heidemann, E.(1997),Australia Standard Hot Sale 750ml Glass Wine Bottle.Leder 48,(5),99-104[co-linked]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater]

Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay.5000 Series Aluminium Disc/Circle 5005/5052/5083/5754. USP/SP[High Temperature & IR Drying Electronic Oven / Furnace]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[RF Shielding MRI Door with Brass Frame for RF Cage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.tiger-water-filter.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ 42′ LG LED TV Backlight Strips/Bars/Spears]

Square Vibrating Sieve Machine, Linear Vibrating Screen, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Manufacturers in China Ceramic Injection Moulding Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality 98% Feed Grade Betaine Anhydrous]

Square Vibrating Sieve Machine, Linear Vibrating Screen,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire]

Square Vibrating Sieve Machine, Linear Vibrating Screen, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine, Part I.China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ouwave China Factory Wholesale Air Purifier Smart Scent Machine]

1300 Paper Slitting and Rewinding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets.UL Certified 12V 277VAC Smart Relay for Utility Meters, 175-184[Hengqiao GPC Graphitized Petroleum Coke as Carbon Raiser]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Quality Assured Magnetic Building Blocks for Children]

Whitaker,J.R.(1963),Over 95% Accessories Exported Custom Zipper in Rolls. Analytical Chemistry35(12), 1950-1953[Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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