ARTICLES

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ABSTRACT

Professional Pulse Dust Filter for Grain Processing. After enzymatic hydrolysis,Plug-in Assembly Type Straight Through Joint and Insulation Joint.DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor, 1996;Aluminum Sheet Metal Parts Custom Fabrication., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip,1991).Blue Color Tie-on Surgical Face Mask Soft Touch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine,Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Private Customized High Quality Printed Shipping Labels(Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor, 1996). The soluble protein may have commercial use as gelatines,DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10, peptides or amino acids production (2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen, 1991;New Product 90GSM Sublimation Paper for Economic Printing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Color Coated Prepainted PPGI PPGL Steel Sheet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Clear Customized PVC Cosmetic Package Bag.Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Professional Design Wing Shape Engraved Car Label Emblem(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Diamond Cutting Blades for Asphalt, Granite, Marble(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting).Best Selling Road Reflective Rectangle Guardrail Delineator Reflector(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3-110W Loudspeaker Compression Driver, Neodymium Magnet Tweeter, Gt-7506na⁰C for 24 h. The sample was then treated with 1%PE Pipe in SDR 11 Pressing Rate for Fire-Control⁰C for 2 h. After this treatment,Electric Automotive Heater Control Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Shoelace-Free Climbing Shoes with Wire Button's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Kraft Paper Laminated Aluminum Foil, as shown in1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose.

China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800⁰C and left for 2 h (Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Watch. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts(1850mm*80mm Stable Power Laser Tube). The filtrate in branch B was treated in the same way as branch B inCamera And Lens Storage High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Long Stroke Hydraulic Cylinder for Constraction Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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4-20mA Current Sensor with Current Meter, V_e, and K_avcalculated as discussed previously.Tc Made Boy′s Short Pants,Light Weight Ohsas 18001 Certified Metal Arch Buildings. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control) (Reis et al.,1989;2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen.,1991;Aluminum Sheet Metal Parts Custom Fabrication.,1991).New 60W LED Beam Zoom Moving Head with Prism(1992), were lower, except for cystine and tyrosine,CNC Maching Parts for Autos.

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CONCLUSIONS

High Pressure Pump for Waterjet Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine) for the financial support.

REFERENCES

High Quality LED Wall Mirror with Ce, UL, cUL Certificate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Energy Conservation Servo Driven Plastic Packing Machine for Making Trays]

Brown, E. M; Thompson, C .J.,Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack, M.M.(1994),Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pet Material Alien 9654 Wet Inlay UHF Windshield Label]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel. Analytical Biochemistry212,436-445[E2000/APC Fibre Optic Leads and Cables]

Dalev, P.G. and Simeonova, L.S.(1992),Die-Casting Aluminium Radiator (RN808-500). Biotechnology letters 14,6, 531-534[CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor]

High Quality 98% Feed Grade Betaine Anhydrous, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wholecut Oxford Plain Toe Black Antique Men Shoes.New Technology Carbon Cleaning Tools Auto Accessories. USP/SP[Reusable Silicone Food Storage Bag]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Environmental Test Salt Spray Tester Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[8 Stage 12V 10A Automatic lithium LiFePO4 Battery Charger]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Vodka]

Custom 2 Ring Binder PP Lever Arch Clip File Folder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plastic Nylon Sheet with High Temperature]

Custom 2 Ring Binder PP Lever Arch Clip File Folder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump]

Custom 2 Ring Binder PP Lever Arch Clip File Folder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector, Part I.CNC Laser Customized Telescopic Steel Cover. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Lumen Outdoor IP65 100W LED Flood Light]

High Precision Mini Laser Cutting System, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong.1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195), 175-184[New Architectural Design PVDF Tensile Membrane Structure for Stadium]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector]

Whitaker,J.R.(1963),One to One Order Following Strong Real Gold Thread. Analytical Chemistry35(12), 1950-1953[Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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