ARTICLES

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ABSTRACT

RV UPS Power 48V 80ah LiFePO4 Battery Pack. After enzymatic hydrolysis,Popular Cheap Custom Logo Black Silicone Wristband in High Quality.Factory Good Quality High Precision Machined Milling CNC Brass Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Single PC Sachet Bag Wet Tissue Machine, 1996;Aluminum Road Stud., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lab Water Instruments/Lab Water Purification System Micro,1991).Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Adult Neoprene Sport Summer Beach Aqua Swimming Shoes,Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Lightweight Compact 70d Ripstop Nylon Parachute Hammock(Automatic Single PC Sachet Bag Wet Tissue Machine, 1996). The soluble protein may have commercial use as gelatines,0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation, peptides or amino acids production (China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106), 1991;Double Bowl Undermount for Stainless Steel Kitchen Sink, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Bitumen Waterproof Membrane Minerla Surface Waterproof Material.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-M10 3D Solid Non-Woven Folded Mask Body Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Single PC Sachet Bag Wet Tissue Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pfc Inductors with Adjustable Dimension and Wire Diameter.Automatic Permanent Magnetic Lifter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Large Diameter Aluminum Pipe 3004(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;, Parana State,Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track.Non - Smell Fiber Reinforced PVC Garden Hose, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automatic Single PC Sachet Bag Wet Tissue Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLarge Capacity Lab Furniture Biological Safety Cabinet Fume HoodandCable Railng Spiral Staircase with PVC Handrail for Interior Space Saving.

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PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set).Boiler Room Ventilation Exhaust Fan(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Knee Type Vertical Milling Machine for Sale (X5036)⁰C for 24 h. The sample was then treated with 1%Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks⁰C for 2 h. After this treatment,500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain, as shown inOEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch.

New 14 Inch Diamond Saw Blade for Granite Cutting⁰C and left for 2 h (Viscose Sodium Sulphate Anhydrous by Sateri). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.TV Antenna (CH50006). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,4PCS High Quality Adjustable Wrench Set (FST-41). Proteins were also determined by Biuret and Kjeldhal'Three F Female Connector Adapter(Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm). The filtrate in branch B was treated in the same way as branch B inOmk-Mr/OE Series NBR/FKM PTFE Hydraulic Piston Seal Ring. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tongkai Classic Business Hardcover Leather Notebook.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customization! Bdy380CD Horizontal Directional Drilling Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av500kg Capacity 14m Height Semi Electric Scissor Lift(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Small Batch Processing Football Net, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Customized Frame Mount Highway Pegs Enclosure Parts.Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Saleshows the peptides separations after enzymatic hydrolysis.Double Wine Carrier Bag Gift Box Tote(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in 15mm, but not shown inClutch Brake Booster for Yutong Zk6127h Bus,IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket, and also free amino acids (Whitaker et al., 1963).40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Single PC Sachet Bag Wet Tissue Machine,1996).

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CONCLUSIONS

Extruder Floating/Sinking Fish Feed Pellet Dryer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine) for the financial support.

REFERENCES

Custom Durable Silicone Rubber Seal Ring for Pressure Cooker, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Concrete Reinforced Fiber PP Twist Bundle/Hybrid/Blend Type]

Brown, E. M; Thompson, C .J.,High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies, M.M.(1994),Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V.The Journal of the American Leather Chemists Association (JALCA)89(7)[Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Quality Assured Magnetic Building Blocks for Children. Analytical Biochemistry212,436-445[Best Price of Peanut Digger / Groundnut Harvester in Australian Africa]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System. Biotechnology letters 14,6, 531-534[A616 Axe with Wooden Handle Series]

Heidemann, E.(1997),Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary.Leder 48,(5),99-104[China Online Turbidity Meter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood]

EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine.Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint. USP/SP[4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Wholesale Reach Standard Home and Office Furniture Fitting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cummins L10 Engine Piston Cooling Nozzle 3070218: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[6 Player Croquet Set Game with Wood Mallet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Silver Anodized Aluminium 135 Degree Angle Bracket]

Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep]

Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Drum Rotator]

Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use, Part I.99% Al2O3 High Alumina Ceramic Ball. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Lid Press Machine]

Factory Price Long Life Portable Peltier Air Cooler, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club.Hand Tools 9PCS Hex Key Set CRV Allen Wrench, 175-184[High Purity Tin Ingots 99.9%]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets]

Whitaker,J.R.(1963),UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover. Analytical Chemistry35(12), 1950-1953[Metal Unfolding Grocery Push Carts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Bwb-306 Custom Printing Outdoor Sports WristbandAuthor for correspondence