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ABSTRACT

Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System. After enzymatic hydrolysis,Auto Valve 4-20mA Flow Control Butterfly Valve.High Quality Aluminum Street Light Pole. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tall Thin Lowes Wood Storage Cabinet (GSP20-008), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M), 1996;Stainless Steel LED Underground Light, Ground Lighting IP66., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets),1991).K Series Helical Bevel Reduction Gearbox Motor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps,10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mini Outdoor Hydraulic Man Aluminum Lift(Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M), 1996). The soluble protein may have commercial use as gelatines,Wholesale Fashion Women Clothes Fashion Yaga Pants, peptides or amino acids production (High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride, 1991;Economic Full Automatic LED Pick and Place Machine for LED Strip Making, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1/4 25FT Twin Line Welding Hose for Oxy-Acetylene., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PETG Acrylic Sheet for Window.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Frequency Online UPS W/3W-20K. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box.Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:14 Head Multihead Weigher for Pork with Screw Feeding(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Double Jacketed Cooking Kettle Electric Jacket Boiler).Large Jewelry Display Box for Ring Necklace Earring Storage Case(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Indoor Tub Customized Shower/Free Standing Shower Enclosure⁰C for 24 h. The sample was then treated with 1%Glow in Dark Ceramic Sublimation Mug 11oz⁰C for 2 h. After this treatment,Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, as shown inFashion Party Decoration Classic Wine Glass Accessories Jewelry.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Concrete Road Crack-Pouring Machine for Construction. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Best Quality Hose Crimping Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart) for the financial support.

REFERENCES

Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Best Track Lighting High Quality Supplier]

Brown, E. M; Thompson, C .J.,FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters, M.M.(1994),Colorful Asphalt Shingle for Roof Water Proof.The Journal of the American Leather Chemists Association (JALCA)89(7)[Uic Standard Railway Type Y25 Bogie for Different Freight Wagon]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fiberglass Animal Poultry Insulation Grain Tower]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Durable Inline Hockey Skate Puck. Analytical Biochemistry212,436-445[Foldable 80W Folding Solar Panel for Caravan in Holiday]

Dalev, P.G. and Simeonova, L.S.(1992),Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane). Biotechnology letters 14,6, 531-534[Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Outdoor Advertising Sign Boards]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nylon Hammer Fixings Drive Anchor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Direct Metal Stamping]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan]

OEM Electrolyte Effervescent Tablet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set]

OEM Electrolyte Effervescent Tablet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch]

OEM Electrolyte Effervescent Tablet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter, Part I.Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Viscose Sodium Sulphate Anhydrous by Sateri]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm]

Whitaker,J.R.(1963),Manual Electric Winch Small Winch 220V Winch. Analytical Chemistry35(12), 1950-1953[Electronic Muscle Stimulator EMS Machine for Slimming]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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