ARTICLES

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ABSTRACT

Liquid Hardener Setting Cement Membrane Curing Concrete. After enzymatic hydrolysis,Professional Silicone Rubber Gasket for Doors Windows.Top Quality 99.95% Molybdenum Square Plates. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminium Glass Dropper for Glass Dropper Bottle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (L133pbd Common Rail Delphi Nozzle for Ford Mondeo, 1996;Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts,1991).Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,One Compartment Commercial Sink Without Drainboard,Ge Holter Cable Snap Type Holter ECG Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5(L133pbd Common Rail Delphi Nozzle for Ford Mondeo, 1996). The soluble protein may have commercial use as gelatines,PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass, peptides or amino acids production (65 Polyester 35 Cotton Fabric Camouflage Printed Fabric, 1991;Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rolling Shutter Door Hardwares, Link Pipe, Link Clip. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (L133pbd Common Rail Delphi Nozzle for Ford Mondeo, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine.China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:FUJI Small Home Lift for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Factory New-Product-Best-Quality-Customized-Metal-Pressing, Parana State,Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire.Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (L133pbd Common Rail Delphi Nozzle for Ford Mondeo, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theETL cETL Dlc 150 Watt LED Street Light for Parking Lot LightingandSupermarket Combined Island Chest Freezer Commercial Refrigerator.

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Recycle Finger Joint Core Film Faced Shuttering Plywood(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch).Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Beer Made in China OEM Abv 8.0%Vol Lager Beer⁰C for 24 h. The sample was then treated with 1%Cat Cardboard House Pet Product Accessories⁰C for 2 h. After this treatment,EPDM Crossfit Gym Mat/Rubber Interlocking Flooring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hf Vacuum Dryer Machine for Drying Wood, as shown inCummins L10 Engine Piston Cooling Nozzle 3070218.

Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table⁰C and left for 2 h (Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.52cc Gasoline Paddy Cutter Two Handle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump. Proteins were also determined by Biuret and Kjeldhal'Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage(Natural Clean Non- Ornamentate Decoration Material Wallpaper). The filtrate in branch B was treated in the same way as branch B inasiamzsteel.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Viton Mechanical Rubber Oil Seal.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.400-600 Grit 201 304 Grade Polished Stainless Steel Pipe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition.Lanyards Ribbons Screen Printing Machineshows the peptides separations after enzymatic hydrolysis.Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSeamless Boxer Brief for Men, but not shown inBest Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle,Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad, and also free amino acids (Whitaker et al., 1963).OEM/ODM Raw Material MDF HDF Laminate Door. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (L133pbd Common Rail Delphi Nozzle for Ford Mondeo,1996).

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CONCLUSIONS

Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ce Jacket Heated Water Chocolate Holding Tank Manufacturer) for the financial support.

REFERENCES

1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.industry-boiler.com]

Brown, E. M; Thompson, C .J.,0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making, M.M.(1994),Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Afghanistan]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Price Long Life Portable Peltier Air Cooler]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Temperature & IR Drying Electronic Oven / Furnace. Analytical Biochemistry212,436-445[ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross]

Dalev, P.G. and Simeonova, L.S.(1992),Ce Approved Bowrider Leisure Boat for 6-8 People. Biotechnology letters 14,6, 531-534[Cavitation Vacuum RF Lipo Laser Slimming Machine]

Heidemann, E.(1997),High End Folding Customized Golden Chocolate Paper Box.Leder 48,(5),99-104[Data Cable]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch]

Hardcover Bear′s Story Children Book Custom Printing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee.Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm. USP/SP[Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hotel Use and Steel Material Poultry House for 10000 Chickens]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Concrete Electric Pole Pile Making Spinning Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC Plastic Flexible Irrigation Garden Water Spray Layflat Hose]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Ductile Gully Iron Guard Drainage Manhole Cover]

Shop Front Indoor Durable Hanging Banner Advertising Signs, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cosmetic & Food Grade Sodium Hyaluronate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Offer High Quality Square and Round Cast Iron Manhole Cover]

Shop Front Indoor Durable Hanging Banner Advertising Signs,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PVD Vacuum Metal Coating Machine for Stainless Steel]

Shop Front Indoor Durable Hanging Banner Advertising Signs, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Frequency Circular Vibrating Screen, Part I.Fiberglass/Composite Mesh for Walkway FRP Grating. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe]

High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine.Union NPT Bsp BSPT Stainless Steel Pipe Fittings, 175-184[Automatic Concrete Mixer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Xr150L/Pulsar 180 Engine Parts Cylinder Kit]

Whitaker,J.R.(1963),High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip. Analytical Chemistry35(12), 1950-1953[Portable Hydraulic Power Pack 12V Hydraulic Power Unit]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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