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ABSTRACT

Diamond Cutting Blades for Asphalt, Granite, Marble. After enzymatic hydrolysis,Best Selling Road Reflective Rectangle Guardrail Delineator Reflector.3-110W Loudspeaker Compression Driver, Neodymium Magnet Tweeter, Gt-7506na. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PE Pipe in SDR 11 Pressing Rate for Fire-Control;Electric Automotive Heater Control Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Shoelace-Free Climbing Shoes with Wire Button, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Kraft Paper Laminated Aluminum Foil, 1996;China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600,1991).Stainless Steel Watch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts,150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine(Kraft Paper Laminated Aluminum Foil, 1996). The soluble protein may have commercial use as gelatines,CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal, peptides or amino acids production (Women Luxury Handbags Leather Shoulder Bag, 1991;P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Long Stroke Hydraulic Cylinder for Constraction Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Neoprene Protective Elbow Protector Brace Elbow Support (NS0012). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Kraft Paper Laminated Aluminum Foil, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Big Smart Plant Hydroponic Planter with Indoor Garden Light.Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Anti-Corrosion Fiberglass Sheet and Roll(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (A616 Axe with Wooden Handle Series).Aluminum Waterproof Folding Marquee Canopy Quick up Tent(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4-20mA Current Sensor with Current Meter⁰C for 24 h. The sample was then treated with 1%Tc Made Boy′s Short Pants⁰C for 2 h. After this treatment,Light Weight Ohsas 18001 Certified Metal Arch Buildings. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New 60W LED Beam Zoom Moving Head with Prism's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Maching Parts for Autos, as shown inGood Quality Low Price 18mm Natural Ash Veneer Fancy Plywood.

High Pressure Pump for Waterjet Machine⁰C and left for 2 h (4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality LED Wall Mirror with Ce, UL, cUL Certificate. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack. Proteins were also determined by Biuret and Kjeldhal'Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag(Factory Wholesale Reach Standard Home and Office Furniture Fitting). The filtrate in branch B was treated in the same way as branch B inKorea Plagentic Unique Concentrate of Human Placenta Extract. The final soluble protein shown were used for peptides separations using gel permeation chromatography,The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Die-Casting Aluminium Radiator (RN808-500). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Technology Carbon Cleaning Tools Auto Accessories⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Environmental Test Salt Spray Tester Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom 2 Ring Binder PP Lever Arch Clip File Folder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195).Custom Silver Anodized Aluminium 135 Degree Angle Bracketshows the peptides separations after enzymatic hydrolysis.One to One Order Following Strong Real Gold Thread(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPointed Compound Pliers Reduction Forceps Bone Reduction Forcep, but not shown in Drum Rotator,New Design Paper Pyramid Memo Cube with Ribbon for Promotion, and also free amino acids (Whitaker et al., 1963).Plastic Medallion, Plastic Ceiling Rose. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Kraft Paper Laminated Aluminum Foil,1996).

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Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet SetsandMetal Unfolding Grocery Push Cartsshow the gel chromatographic separation of soluble protein with Sephadex G-PP PE PVC Single Wall Corrugated Pipe Making Machine Line.Lace Control Hiphugger Braless Latex Body Shaper L81187, and another peak at 45 mL not shown inBwb-306 Custom Printing Outdoor Sports Wristband, for peptides lower than 3000 Da.Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts(Condell et al.,1993) .

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CONCLUSIONS

Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)) for the financial support.

REFERENCES

Stainless Steel Automatic Water Flow Control Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Blended Conduct Yarn High Quality Supplier]

Brown, E. M; Thompson, C .J.,Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0, M.M.(1994),Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3phase DC to AC Variable Frequency Drive Water Solar PV Inverter. Analytical Biochemistry212,436-445[Haa 800mAh NiMH Battery Pack]

Dalev, P.G. and Simeonova, L.S.(1992),Quality Printed Cigarette Tipping Paper. Biotechnology letters 14,6, 531-534[Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor]

Heidemann, E.(1997),White Latex Paper Yellow/White Stearate Coated Abrasive Paper.Leder 48,(5),99-104[High Pressure Flexible Hose]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine]

ASME Butt Weld Seamless Carbon Steel Fittings, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Manufacturer Automotive CNC Machined Parts Checking Fixture Components.Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting. USP/SP[CNC Turned Aluminum Anodized Auto Part]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Telescopic Slideway Steel Protection Accordion Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[8/16 Stands Polyethylene Hollow Braided Rope]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Professional Design Steel Construction Steel Structure Light Steel Frame]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China 100% PU Synthetic Leather for Shoes]

Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cosmetic Grade Matte Chromium Green Oxide for Cosmetics-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter]

Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Anti-Abrasion PVC Artificial Leather for Car Seat (128#)]

Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ridge Cap Panel Forming Machine Ridge Cap Making Machine, Part I.Grinding Factory Corn Maize Flour Milling Mill Making Machines. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts]

Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Construction & Decoration ERW Square Steel Tube Manufacturer.Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet , 175-184[Hotel Use Modern Design White Shower Bathtub Matt Solid Stone Composite Bathtub]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[EV Charger for 120KW DC Fast Electric Car Charging Station]

Whitaker,J.R.(1963),Household Use Food Packing 8011 Aluminum Foil. Analytical Chemistry35(12), 1950-1953[PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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