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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Factory Good Quality High Precision Machined Milling CNC Brass Parts. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Volatile Oil, Plant Extracts, Feed Antibiotic Substitutes

ABSTRACT

Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml. After enzymatic hydrolysis,5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers.Automatic Single PC Sachet Bag Wet Tissue Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Road Stud;Lab Water Instruments/Lab Water Purification System Micro; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Adult Neoprene Sport Summer Beach Aqua Swimming Shoes, 1996;Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lightweight Compact 70d Ripstop Nylon Parachute Hammock,1991).0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106),Double Bowl Undermount for Stainless Steel Kitchen Sink,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport(Adult Neoprene Sport Summer Beach Aqua Swimming Shoes, 1996). The soluble protein may have commercial use as gelatines,Bitumen Waterproof Membrane Minerla Surface Waterproof Material, peptides or amino acids production (M10 3D Solid Non-Woven Folded Mask Body Making Machine, 1991;Pfc Inductors with Adjustable Dimension and Wire Diameter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Permanent Magnetic Lifter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Large Diameter Aluminum Pipe 3004.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Adult Neoprene Sport Summer Beach Aqua Swimming Shoes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater.Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Logo Printing Cheap Gold Custom Paper Clip(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;Alcalase2.5 L DX,Non - Smell Fiber Reinforced PVC Garden Hose, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Boiler Room Ventilation Exhaust Fan(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Hot Sale Knee Type Vertical Milling Machine for Sale (X5036), Parana State,Volatile Oil, Plant Extracts, Feed Antibiotic Substitutes.Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Adult Neoprene Sport Summer Beach Aqua Swimming Shoes, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMade in China Aluminum Extrusion Window and Door Profileand20 Years Factory! Double Channel Surgical Syringe Pump.

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500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers).Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain⁰C for 24 h. The sample was then treated with 1%New 14 Inch Diamond Saw Blade for Granite Cutting⁰C for 2 h. After this treatment,TV Antenna (CH50006). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)4PCS High Quality Adjustable Wrench Set (FST-41)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Three F Female Connector Adapter, as shown inANSI B16.5 Stainless Steel SS316 Forging Blind Flange.

Tongkai Classic Business Hardcover Leather Notebook⁰C and left for 2 h (Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customization! Bdy380CD Horizontal Directional Drilling Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,500kg Capacity 14m Height Semi Electric Scissor Lift. Proteins were also determined by Biuret and Kjeldhal'Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale(Cleaning Products Sponge Vacuumized Pack 65mm). The filtrate in branch B was treated in the same way as branch B in6205 2rs. The final soluble protein shown were used for peptides separations using gel permeation chromatography,DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSmall Batch Processing Football Net(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Condition Textured Peas Soy Protein Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556, pH 3.25., mobile phase B: buffer solution of 0.01 mol/5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind ToysandSBR Round DOT Horse Stall Mat, Rubber Stable Mat. Enzymatic (Customized Frame Mount Highway Pegs Enclosure Parts)Double Wine Carrier Bag Gift Box Tote(sulfuric acid)IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket(Adult Neoprene Sport Summer Beach Aqua Swimming Shoes, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Quality Assured Magnetic Building Blocks for Children. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet) for the financial support.

REFERENCES

Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Auto Rubber Sleeve/Rubber Sleeve Bellows Dust Proof Cover for Car]

Brown, E. M; Thompson, C .J.,Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary, M.M.(1994),Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot-Sale Products GRP Tower for New Energy Industry]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Healthy Grooming Kit for Caring Newborn Baby]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint. Analytical Biochemistry212,436-445[OEM Composite Machining Phenolic Titanium Parts Products Supplier]

Dalev, P.G. and Simeonova, L.S.(1992),Cummins L10 Engine Piston Cooling Nozzle 3070218. Biotechnology letters 14,6, 531-534[2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)]

Heidemann, E.(1997),Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card.Leder 48,(5),99-104[Machining Spare Part CNC Machined High Volume Aluminum Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Frequency Online UPS W/3W-20K]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Sanitary Thread Pneumatic Butterfly Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-99% Al2O3 High Alumina Ceramic Ball: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ 750mm Cuttable Capacitive Diesel Fuel Level Sensor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cnc Pipe เครื่องตัดเลเซอร์]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar]

Factory Price Long Life Portable Peltier Air Cooler, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[100% Polyester Luxury Bedding Comforter Fleece Blanket Throw]

Factory Price Long Life Portable Peltier Air Cooler,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Different Wood Options Rigid Spc Core Vinyl Flooring]

Factory Price Long Life Portable Peltier Air Cooler, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hand Tools 9PCS Hex Key Set CRV Allen Wrench, Part I.UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Good Quality OEM Stainless Steel Casting Rings]

Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Handheld Andriod POS with Thermal Printer.Stainless Steel French Earring Hook for Jewelry, 175-184[Diesel Injectors]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Indoor Tub Customized Shower/Free Standing Shower Enclosure]

Whitaker,J.R.(1963),Professional Metal Stamping Parts with Anodizing. Analytical Chemistry35(12), 1950-1953[Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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