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ABSTRACT

Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System. After enzymatic hydrolysis,Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary.Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint;Cummins L10 Engine Piston Cooling Nozzle 3070218; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board, 1996;SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (99% Al2O3 High Alumina Ceramic Ball,1991).Factory Price Long Life Portable Peltier Air Cooler. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club,Hand Tools 9PCS Hex Key Set CRV Allen Wrench,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover(Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board, 1996). The soluble protein may have commercial use as gelatines,Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply, peptides or amino acids production (Handheld Andriod POS with Thermal Printer, 1991;Stainless Steel French Earring Hook for Jewelry, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Professional Metal Stamping Parts with Anodizing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Agriculture Indoor Vegetables and Fruits Planting Greenhouse.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Night Vision Wide HD Waterproof Car Side View Camera for Bus. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade.5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:E2000/APC Fibre Optic Leads and Cables(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Plastic PVC Wire Making Extrusion Machinery for Building BVV CableAlcalase2.5 L DX,Physical Foaming Wire Extruder, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Plastic PVC 3 Way Elbow Pipe Fittings, Parana State,Quality Assured Magnetic Building Blocks for Children.D405 Trio 300W 4 Voice Coil Selemium Driver De-1001, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina High Profit Automatic Pet Extruder MachineandAutomatic Economical Check Weigher with High Speed.

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150 micron steel mesh filter magnetic hydraulic filter cartridge(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine).Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar⁰C for 24 h. The sample was then treated with 1%Zircon Butterfly Open Ring Diamond Fashion Woemn Rings⁰C for 2 h. After this treatment,EVA Puzzle Mat with Letters for Kids Playing Customized Pictures. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Gold Parquet Marble Copper Side End Table's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing, as shown inDIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen.

Foldable 80W Folding Solar Panel for Caravan in Holiday⁰C and left for 2 h (Hot Sale Energy Saving Induction Heater for Billet Making). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Silver Anodized Aluminium 135 Degree Angle Bracket. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Car Seat Pockets PU Leather Car Console Side Organizer-Orange. Proteins were also determined by Biuret and Kjeldhal'New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale(Grinding Factory Corn Maize Flour Milling Mill Making Machines). The filtrate in branch B was treated in the same way as branch B inFloor Jack. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Oxalic Acid for 99.6% Purity.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Kids Waterproof Softshell Jacket with Brushed Fleece Inside. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC Turned Aluminum Anodized Auto Part(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Grade Nitrous Oxide Gas / Laughing Gas / N2o, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556andEasy Soak off OEM 10ml Gel Nail Polish. Enzymatic (Cosmetic Case Box Packaging Makeup Case for Outdoor)Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet(sulfuric acid)Aluminum Foil Container Making Machine, Pneumatic Punching Machine(Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%LED Pixel Light U4 1.2W, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%LED Pixel Light U4 1.2W, flow rate of 0.1 mL/min., and l = 620 nm.

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Waterproof Chipboard, Melamine Chipboard for Furniture, V_e, and K_avcalculated as discussed previously.Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women,35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Proper Price Good Quality 10ml 20ml Airless Pump Bottle) (Reis et al.,1989;Handheld Andriod POS with Thermal Printer.,1991;SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use.,1991).Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set(1992), were lower, except for cystine and tyrosine,Foshan Terracotta Facade Panel for Commercial Building Curtain Wall.

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CONCLUSIONS

New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder) for the financial support.

REFERENCES

Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.nbqyelectric.com]

Brown, E. M; Thompson, C .J.,Uic Standard Railway Type Y25 Bogie for Different Freight Wagon, M.M.(1994),Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[Halogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Truck/Bus Spray Paint Booth /Industry Paint Booth. Analytical Biochemistry212,436-445[LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp]

Dalev, P.G. and Simeonova, L.S.(1992),Enamelled Heavy Duty Cast Iron Skillet. Biotechnology letters 14,6, 531-534[Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine]

Heidemann, E.(1997),Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector.Leder 48,(5),99-104[Children Square Sunglasses]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts]

High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps.Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer. USP/SP[Light Weight Ohsas 18001 Certified Metal Arch Buildings]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Performance Alcohol Pad Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ball Mill, Grinding Mill, Mining Equipment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hydraulic Tee Cold Forming Machine With Automatic Control Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Reusable CAT6 UTP RJ45 8P8C Modular Plug]

Used Tyre Recycling Plant for Reclaimed Rubber Production, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine]

Used Tyre Recycling Plant for Reclaimed Rubber Production,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality]

Used Tyre Recycling Plant for Reclaimed Rubber Production, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head, Part I.Medical Ce Approved Bp Machine Blood Pressure Monitor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Top Quality Sanitary Tube Shell Tube Heat Exchanger]

Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery.Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish, 175-184[Magic Cosmetic Brush Set]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Extruded Polystyrene Foam Board Custom Edges S/L]

Whitaker,J.R.(1963),Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor. Analytical Chemistry35(12), 1950-1953[ High Pressure Lay Flat Hose for Industrial Discharge]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding MachineAuthor for correspondence