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ABSTRACT

Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings. After enzymatic hydrolysis,Square Vibrating Sieve Machine, Linear Vibrating Screen.Manufacturers in China Ceramic Injection Moulding Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine;China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1300 Paper Slitting and Rewinding Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets, 1996;UL Certified 12V 277VAC Smart Relay for Utility Meters., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Over 95% Accessories Exported Custom Zipper in Rolls,1991).Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China 100% PU Synthetic Leather for Shoes,HD Video Wall P3.91 LED Panel Display Indoor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM Metal Stamping Stainless Steel Parts with CNC Machining(High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets, 1996). The soluble protein may have commercial use as gelatines,FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure, peptides or amino acids production (Epoxy Resins, 1991;Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;24 Cores MPO/MTP Cassette., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Concrete Paver Interlocking Brick Block Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lightweight Design Industrial Robot with Six Axis for Painting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Small Diameter Coil Compression Spring From Factory.FDA Standard BBQ Skewer with Colorful Wooden handle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005, Parana State,5000 Series Aluminium Disc/Circle 5005/5052/5083/5754.Metal Unfolding Grocery Push Carts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theClothing Hard Tag Security Tag for Supermarket/Retail Storeand100% Hand-Painted Canvas Oil Painting Wall Art for Trees.

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Auto/Manual Spray Painting Booth/Cabinet with Best Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Neoprene Protective Elbow Protector Brace Elbow Support (NS0012)).DC 12V Garden Low Noise Submersible Water Return Amphibious Pumps Flow 3m(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 Dotted Navy Rucksack Children School Bag Cotton Backpack⁰C for 24 h. The sample was then treated with 1%China Manufacturer Multi Span Polycarbonate Industrial Green House⁰C for 2 h. After this treatment,Custom Plastic Injected (injection) Parts Molding Process. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)4mm 6mm2 Soler Extension Leads with Mc4's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)E0303 300n Activator Chiropractic Impulse Adjusting Instrument, as shown inU Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud.

Men′s Black and Grey Stripe Bamboo Dress Sock⁰C and left for 2 h (P1.25 Full Color Cable Less Indoor Fixed LED Display). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Part. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Customized High Quality Stainless Steel Precision Motorcycle Parts /Accessory Sample. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Mobile Phone Shop Display Furniture Interior Design(Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine). The filtrate in branch B was treated in the same way as branch B inHf Rfid Sticker. The final soluble protein shown were used for peptides separations using gel permeation chromatography,En71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRubber Seal for Crank Box of Hks Warp Knitting Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industrial High  Temperature  Resistant Smooth Flat Wire Power Cable⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Fashion TPU Mobile Cover Case for Huawei, Leather Phone Case for Huawei Mate 20, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Medical Grade Ventilator for Sleep Apnea Breathing Machine, V_e, and K_avcalculated as discussed previously.UV Resistant Single Component Water Based Polyurethane Waterproof Coating,Steel Rubber Seal for Dust Proof. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine) (Reis et al.,1989;Epoxy Resins.,1991;UL Certified 12V 277VAC Smart Relay for Utility Meters.,1991).Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf(1992), were lower, except for cystine and tyrosine,Kindergarten Playground Preschool Kids Plastic Slide and Swing.

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CONCLUSIONS

W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality 3000L Composting Machine with Lower Price) for the financial support.

REFERENCES

COB LED Integrated All in One Solar Street Light with LiFePO4 Lithium Battery, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Hot Linear Sieve Vibrating Screen Coffee Separator]

Brown, E. M; Thompson, C .J.,High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou, M.M.(1994),New Virgin Teflon PTFE Teflon Gaskets/Back up Rings.The Journal of the American Leather Chemists Association (JALCA)89(7)[17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[HAIRUI 850A+ 600W SMD Rework Soldering Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Capacitive Push Button Membrane Keyboards. Analytical Biochemistry212,436-445[2019 Oversized Metal Frame Cat Eye Women Fashion Sunglasses]

Dalev, P.G. and Simeonova, L.S.(1992),Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow. Biotechnology letters 14,6, 531-534[Enamelled Heavy Duty Cast Iron Skillet]

Heidemann, E.(1997),High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss.Leder 48,(5),99-104[Pet and Animal Food Extrusion Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Steel Rubber Seal for Dust Proof]

12V 96LED Outdoor Lighting Solar Torch Light for Christmas, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automotive ECU Wire Harness 24 Pin Grey Connector]

12V 96LED Outdoor Lighting Solar Torch Light for Christmas,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve]

12V 96LED Outdoor Lighting Solar Torch Light for Christmas, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:SMF/VRLA Lead Acid Battery AGM Storage Battery for UPS System VRLA 12V200ah Export to UAE, India, Bangladesh, Afghanistan, Pakistan, Africa, Part I.High Efficiency Swimming Pool Air Source Water Heat Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Durable Inline Hockey Skate Puck]

CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan.Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box, 175-184[Lanboo 16mm/19mm/22mm/25mm LED Indicator Light with 12V/24V/110V/220V]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China High Profit Automatic Pet Extruder Machine]

Whitaker,J.R.(1963),QJ-M Moving small manual brick making machine with low cost. Analytical Chemistry35(12), 1950-1953[Automatic Economical Check Weigher with High Speed]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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