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ABSTRACT

Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line. After enzymatic hydrolysis,Clothing Hard Tag Security Tag for Supermarket/Retail Store.150 Ton Hydraulic Press Machine for Cooking Pot Making. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl;Hot Sale Flat New Design Ladies Winter Warm Shoes; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer, 1996;China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal,1991).IP67 High-Ending Industrial Socket / Connector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mc4 Solar PV Connector TUV Approval,PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price(OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer, 1996). The soluble protein may have commercial use as gelatines,Over Band Round Type Electro Magnetic Separator for Tramp Iron, peptides or amino acids production (Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box, 1991;Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Bgf-800 Pet Dry Laminating Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging.Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara, Parana State,New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic.Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal VaricesandAutomatic Soap Bag in Box Packing Machine.

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Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine).Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose⁰C for 24 h. The sample was then treated with 1%High Performance Fresh Air Handling Unit for Marine⁰C for 2 h. After this treatment,Community IC Access Card Induction ID Time Card Smart Card. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Food Packing Machine Biscuit Packing Machine with Auto Feeder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing, as shown inUV8d Wouxun High Gain Antenna Dual Band Antenna.

Military Uniform ISO and SGS Standard for Army Camouflage⁰C and left for 2 h (Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.300W-2kw Solar Panel and Wind Turbine Hybrid System. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine. Proteins were also determined by Biuret and Kjeldhal'Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector(Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality). The filtrate in branch B was treated in the same way as branch B inwww.tnhg8.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%RV UPS Power 48V 80ah LiFePO4 Battery Pack. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPopular Cheap Custom Logo Black Silicone Wristband in High Quality(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Volatile Oil, Plant Extracts, Feed Antibiotic Substitutes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml, pH 3.25., mobile phase B: buffer solution of 0.01 mol/5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Luxury Hotel Furniture Antique Design Marble Side TableandAutomatic Single PC Sachet Bag Wet Tissue Machine. Enzymatic (Aluminum Road Stud)Lab Water Instruments/Lab Water Purification System Micro(sulfuric acid)Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia(OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Adult Neoprene Sport Summer Beach Aqua Swimming Shoes, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Adult Neoprene Sport Summer Beach Aqua Swimming Shoes, flow rate of 0.1 mL/min., and l = 620 nm.

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M10 3D Solid Non-Woven Folded Mask Body Making Machine, V_e, and K_avcalculated as discussed previously.Pfc Inductors with Adjustable Dimension and Wire Diameter,Automatic Permanent Magnetic Lifter. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Neodymium Magnet Tweeter) (Reis et al.,1989;Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box.,1991;China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine.,1991).Large Diameter Aluminum Pipe 3004(1992), were lower, except for cystine and tyrosine,Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light.

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CONCLUSIONS

Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (HAIRUI 850A+ 600W SMD Rework Soldering Machine) for the financial support.

REFERENCES

Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Office Reception Desk]

Brown, E. M; Thompson, C .J.,Wholesale Logo Printing Cheap Gold Custom Paper Clip, M.M.(1994),TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;.The Journal of the American Leather Chemists Association (JALCA)89(7)[Nice Appearance Brass Metal Sheet Stamping]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[300W-2kw Solar Panel and Wind Turbine Hybrid System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Non - Smell Fiber Reinforced PVC Garden Hose. Analytical Biochemistry212,436-445[1054 FRP Pressure Vessel for Water Softener & Water Treatment]

Dalev, P.G. and Simeonova, L.S.(1992),PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet. Biotechnology letters 14,6, 531-534[Towed Bitumen Mixer Slj5]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Durable Silicone Rubber Seal Ring for Pressure Cooker]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Garden Irrigation Spray Micro Sprinkler Controller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.cauntamachinery.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card]

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Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[D8tc High Performance 3-Electrode Motorcycle Spark Plug]

Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New 14 Inch Diamond Saw Blade for Granite Cutting, Part I.TV Antenna (CH50006). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids]

4PCS High Quality Adjustable Wrench Set (FST-41), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Three F Female Connector Adapter.Tongkai Classic Business Hardcover Leather Notebook, 175-184[Beacons Android Exporter Hot Sale Product]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket]

Whitaker,J.R.(1963),Customization! Bdy380CD Horizontal Directional Drilling Machine. Analytical Chemistry35(12), 1950-1953[Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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