ARTICLES

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ABSTRACT

High Speed Mixer for Plastic Extrusion Line. After enzymatic hydrolysis,High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger.10mm Gold Plated Stainless Steel Balls G100 Polish Ball. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine;Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.304 Seamless Stainless Steel Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Manual 3-Way Plug Valve, 1996;Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck,1991).Natural Clean Non- Ornamentate Decoration Material Wallpaper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder,Super Soft 100% Viscose From Bamboo Bedding Set,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Theme Jungle Plastic Stainless Steel Slide Outdoor Playground(Stainless Steel Manual 3-Way Plug Valve, 1996). The soluble protein may have commercial use as gelatines,Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking, peptides or amino acids production (Updated Stylish Peristaltic Biogas Pump Gas Booster Pump, 1991;110V-240V LED Filament Bulb Globe Cafe Patio String Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Professional Sound System Vt215 Line Array Speaker., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Manual 3-Way Plug Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Duct Evaporative Air Cooler for Warehouse Factory.Micro Sprinkler for Water Saving Irrigation-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Manual Glass Lifting Machine for Making Double Glass, Parana State,Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine.Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Manual 3-Way Plug Valve, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching PlantandAirtac Tubing Quick Connect Puick Coupling Pneumatic Fitting.

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Towed Bitumen Mixer Slj5(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cog Mono Round Display LCD Module).High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3D Inkjet Printing Waterproof Interior Kitchen Wall Tile⁰C for 24 h. The sample was then treated with 1%IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC⁰C for 2 h. After this treatment,T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Viscose Sodium Sulphate Anhydrous by Sateri, as shown inWholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric.

Orange Silica Gel 2-4 mm with High Quality⁰C and left for 2 h (Tfl Blue Rubber Transmission Belts/Automotive Spare Part). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,aluminium discs for fry pan alloy 1060 1100 1050 3003. Proteins were also determined by Biuret and Kjeldhal'High Quality Insulated Double Wall Bamboo Glass Water Bottle(150 micron steel mesh filter magnetic hydraulic filter cartridge). The filtrate in branch B was treated in the same way as branch B inwww.wzzy-spring.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aroma Reed Diffuser with Ratten Sticks for Home Fragrance.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Fashion Ladies Backpack PU Leather Backpack. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSuction Filter Cylinder Match 48 Series Filter Brands(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.16oz Square Shape Colors Glass Mason Jar with Embossed Design⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruderand100ml Plastic E Liquid Vape Cig Juice Dropper Bottle. Enzymatic (Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building)Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags(sulfuric acid)2-25mm Co-Extruded Highlight PVC Foam Board(Stainless Steel Manual 3-Way Plug Valve, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CNC Engraving Machine Laser Marking for Assembly Line, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CNC Engraving Machine Laser Marking for Assembly Line, flow rate of 0.1 mL/min., and l = 620 nm.

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Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts, V_e, and K_avcalculated as discussed previously.Zoo-Tunes Portable Wireless Mini Character Speakers,CF8m Air Actuated Valve Dn15. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Boiler Room Ventilation Exhaust Fan) (Reis et al.,1989;Updated Stylish Peristaltic Biogas Pump Gas Booster Pump.,1991;Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine.,1991).Rectangular Design Ceramic Small Glass Basin Sink for Bathroom(1992), were lower, except for cystine and tyrosine,Premium Baby Toddler Food Feeding Silicone Bowl with Lid.

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CONCLUSIONS

Hardcover Bear′s Story Children Book Custom Printing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press) for the financial support.

REFERENCES

Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Flue Gas Plate Heat Exchanger Recuperator]

Brown, E. M; Thompson, C .J.,Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire, M.M.(1994),Liquid Hardener Setting Cement Membrane Curing Concrete.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality X Ray Lead Glasses with Good Prices]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Professional Silicone Rubber Gasket for Doors Windows. Analytical Biochemistry212,436-445[Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Top Quality 99.95% Molybdenum Square Plates. Biotechnology letters 14,6, 531-534[Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp]

Heidemann, E.(1997),HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee.Leder 48,(5),99-104[World Cup Hot Sale S925 Football Style Sterling Silver Pendant Necklace Jewelry Pendant Jewelry]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[8FT Wood Log Debarker Machine for Plywood Machinery]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-L133pbd Common Rail Delphi Nozzle for Ford Mondeo: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[LSAW Steel Pipe Piles / Longitudinal Seam Submerged Arc Welded Steel Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft]

Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting]

Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[White Latex Paper Yellow/White Stearate Coated Abrasive Paper]

Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine, Part I.One Compartment Commercial Sink Without Drainboard. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass]

Ge Holter Cable Snap Type Holter ECG Cable, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5.PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass, 175-184[Biochemical Inspection]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Speed Rope Weaving Machine]

Whitaker,J.R.(1963),65 Polyester 35 Cotton Fabric Camouflage Printed Fabric. Analytical Chemistry35(12), 1950-1953[All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM Custom Design Print Cotton Microfiber Travel Beach Towels ManufacturerAuthor for correspondence