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ABSTRACT

Fake Fish Luminescent Tropical Fish Fish Tank Decoration. After enzymatic hydrolysis,Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine.High Pressure Die Casting Aluminum Die Casting Gear Box Housing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Small Portable Air Water Purifier with Disinfection and Detoxification, 1996;Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (1850mm*80mm Stable Power Laser Tube,1991).White Color Premium Quality Disposable Plastic PVC Apron. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Galvanized Bar Grating,Ring Die Bio Organic Fertilizer Pellet Mill Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Dipped Galvanized Deer Fence From Factory(Small Portable Air Water Purifier with Disinfection and Detoxification, 1996). The soluble protein may have commercial use as gelatines,High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce, peptides or amino acids production (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, 1991;Cheap Customized Aluminium Sand Casting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Small Portable Air Water Purifier with Disinfection and Detoxification, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve.High Quality RV Electric Seating Recliner-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Foshan Terracotta Facade Panel for Commercial Building Curtain Wall).304 Stainless Steel Expansion Anchor Bolt(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Small Plug-in Insect Killer Mosquito Killing Lamp⁰C for 24 h. The sample was then treated with 1%Wholesale Customize PU Leather Hardcover Notebook with Window⁰C for 2 h. After this treatment,Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer, as shown inNew Virgin Teflon PTFE Teflon Gaskets/Back up Rings.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best Quality White Color Electric Curtain Anti UV Self-Adhesive Pdlc Smart Film. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear, pH 3.25., mobile phase B: buffer solution of 0.01 mol/FTTH Optical Network Terminal for Gpon ONU and CATV, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Supreme 524 Mv Sea Kayak) for the financial support.

REFERENCES

2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[86 Inch Interactive Flat Panel]

Brown, E. M; Thompson, C .J.,IP67 High-Ending Industrial Socket / Connector, M.M.(1994),Mc4 Solar PV Connector TUV Approval.The Journal of the American Leather Chemists Association (JALCA)89(7)[Carbon Dioxide Gas with 50L Seamless Cylinder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Product 90GSM Sublimation Paper for Economic Printing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China. Analytical Biochemistry212,436-445[China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons]

Dalev, P.G. and Simeonova, L.S.(1992),Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price. Biotechnology letters 14,6, 531-534[24 Cores MPO/MTP Cassette]

Heidemann, E.(1997),Over Band Round Type Electro Magnetic Separator for Tramp Iron.Leder 48,(5),99-104[Automatic Heat Hot POF Shrink Shrinkable Shrinking Wrap Wrapping Pack Package Packing Packaging Film Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price]

China Durable 25kg 50kg Red PP Woven Bag Sack, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box.Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine. USP/SP[High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pre-Seasoned Cast Iron 5-Piece Cookware Set]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles]

Bgf-800 Pet Dry Laminating Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[19gauge Galvanized Twin Binding Wire]

Bgf-800 Pet Dry Laminating Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CE Certificated Wheel Loader (HQ912) with Agricultural Tire]

Bgf-800 Pet Dry Laminating Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging, Part I.Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mc4 Solar PV Connector TUV Approval]

New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Polyester Cocoon Bobbins Thread for Household Textiles.Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood, 175-184[China Manufacturer Industrial Aluminum Floor Bar Grating]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Jelly Cup]

Whitaker,J.R.(1963),HAIRUI 850A+ 600W SMD Rework Soldering Machine. Analytical Chemistry35(12), 1950-1953[Fashion 925 Sterling Silver Pendants Necklace Jewelry]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Fgg 2b 304 Metal Circular Push Pull ConnectorAuthor for correspondence