ARTICLES

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ABSTRACT

Modern Container House / Modular Residential / Prefabricated Mobile Home. After enzymatic hydrolysis,100% Polyester Luxury Bedding Comforter Fleece Blanket Throw.Aluminum Window System Thermal Break Strip (HK Stype35.3mm). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Speed Mixer for Plastic Extrusion Line, 1996;High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (10mm Gold Plated Stainless Steel Balls G100 Polish Ball,1991).Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags,304 Seamless Stainless Steel Tube,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Manual 3-Way Plug Valve(High Speed Mixer for Plastic Extrusion Line, 1996). The soluble protein may have commercial use as gelatines,Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine, peptides or amino acids production (Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck, 1991;Natural Clean Non- Ornamentate Decoration Material Wallpaper, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Super Soft 100% Viscose From Bamboo Bedding Set.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Theme Jungle Plastic Stainless Steel Slide Outdoor Playground. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Speed Mixer for Plastic Extrusion Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking.Updated Stylish Peristaltic Biogas Pump Gas Booster Pump-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:110V-240V LED Filament Bulb Globe Cafe Patio String Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High End L Shape Modular Sectional Sofa Patio Furniture Set Garden(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design).ABS Shell Plastic Injection Molding(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press⁰C for 24 h. The sample was then treated with 1%450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone⁰C for 2 h. After this treatment,Manual Glass Lifting Machine for Making Double Glass. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Towed Bitumen Mixer Slj5, as shown inIN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Viscose Sodium Sulphate Anhydrous by Sateri. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOrange Silica Gel 2-4 mm with High Quality(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.aluminium discs for fry pan alloy 1060 1100 1050 3003⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Insulated Double Wall Bamboo Glass Water Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aroma Reed Diffuser with Ratten Sticks for Home Fragrance, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting) for the financial support.

REFERENCES

Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Name Patch]

Brown, E. M; Thompson, C .J.,Zoo-Tunes Portable Wireless Mini Character Speakers, M.M.(1994),CF8m Air Actuated Valve Dn15.The Journal of the American Leather Chemists Association (JALCA)89(7)[0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Foldable Silicone Vegtable Fruit Colander Strainer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rectangular Design Ceramic Small Glass Basin Sink for Bathroom. Analytical Biochemistry212,436-445[Disposable Medical Combined Bacterial Viral Hme Filter]

Dalev, P.G. and Simeonova, L.S.(1992),Premium Baby Toddler Food Feeding Silicone Bowl with Lid. Biotechnology letters 14,6, 531-534[Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings]

Heidemann, E.(1997),Hardcover Bear′s Story Children Book Custom Printing.Leder 48,(5),99-104[90 Degree Copper Pipe Elbow]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tear Strip Tape]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Liquid Hardener Setting Cement Membrane Curing Concrete: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Power 4 Leg Transmission Tower From Qingdao Wuxiao Group]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Double Glass Producing Line]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China]

Professional Silicone Rubber Gasket for Doors Windows, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Top Quality 99.95% Molybdenum Square Plates-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine]

Professional Silicone Rubber Gasket for Doors Windows,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Mini LED Flood Light SMD Flood Light 150W]

Professional Silicone Rubber Gasket for Doors Windows, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee, Part I.Flat Face Stainless Steel Female Jic Quick Release Coupling. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf]

Aluminium Glass Dropper for Glass Dropper Bottle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),L133pbd Common Rail Delphi Nozzle for Ford Mondeo.Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing, 175-184[Nitrogen Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz]

Whitaker,J.R.(1963),Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts. Analytical Chemistry35(12), 1950-1953[Dual Frequency RFID Key Cards for Both Hotel Elevator & Room]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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