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ABSTRACT

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Key words;Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine;High Pressure Die Casting Aluminum Die Casting Gear Box Housing; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove, 1996;CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Small Portable Air Water Purifier with Disinfection and Detoxification,1991).Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1850mm*80mm Stable Power Laser Tube,White Color Premium Quality Disposable Plastic PVC Apron,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Galvanized Bar Grating(Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove, 1996). The soluble protein may have commercial use as gelatines,Ring Die Bio Organic Fertilizer Pellet Mill Machine, peptides or amino acids production (Hot Dipped Galvanized Deer Fence From Factory, 1991;High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cheap Customized Aluminium Sand Casting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line.Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Supreme 524 Mv Sea Kayak, Parana State,China Durable 25kg 50kg Red PP Woven Bag Sack.API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWhite Color Premium Quality Disposable Plastic PVC ApronandAluminum Road Stud.

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1-40mm Thickness and 1.22m, 1.56m Size PVC Celuka Board Manufacturer for Cabinets(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Design Disposable Trocars for Laparoscopic Surgery).High Definition Outdoor Video Advertising LED Billboard(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm⁰C for 24 h. The sample was then treated with 1%304 Stainless Steel Expansion Anchor Bolt⁰C for 2 h. After this treatment,New Small Plug-in Insect Killer Mosquito Killing Lamp. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Customize PU Leather Hardcover Notebook with Window's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11), as shown inDurable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage.

Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper⁰C and left for 2 h (Customizable Logo Plastic Safety Walking Reflectors Chinese Factory). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons. Proteins were also determined by Biuret and Kjeldhal'OEM Composite Machining Phenolic Titanium Parts Products Supplier(Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet). The filtrate in branch B was treated in the same way as branch B inIndustry Basket Washing Machine Crate Washer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCoil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Xtsky Automotive Wheel Hub Bearing (DAC37720037), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (100% Hand-Painted Canvas Oil Painting Wall Art for Trees) for the financial support.

REFERENCES

OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rice Reaper Head 4gl150 for 4 Wheel Tractor]

Brown, E. M; Thompson, C .J.,China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine, M.M.(1994),2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal.The Journal of the American Leather Chemists Association (JALCA)89(7)[2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-IP67 High-Ending Industrial Socket / Connector. Analytical Biochemistry212,436-445[4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes]

Dalev, P.G. and Simeonova, L.S.(1992),Mc4 Solar PV Connector TUV Approval. Biotechnology letters 14,6, 531-534[Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry]

Heidemann, E.(1997),PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China.Leder 48,(5),99-104[Most Popular Cone Crusher]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Delrin Sheets with Good Wear Resistance]

Funny Kids Soft Play Maze Big Slide Indoor Soft Playground, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price.Over Band Round Type Electro Magnetic Separator for Tramp Iron. USP/SP[New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tubular Steam Heater]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Supermarket Combined Island Chest Freezer Commercial Refrigerator]

Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OEM Viton Mechanical Rubber Oil Seal]

Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch]

Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Bgf-800 Pet Dry Laminating Machine, Part I.Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cummins L10 Engine Piston Cooling Nozzle 3070218]

Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating.New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine, 175-184[Air Compressor Tanks For Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet]

Whitaker,J.R.(1963),Polyester Cocoon Bobbins Thread for Household Textiles. Analytical Chemistry35(12), 1950-1953[Natural Clean Non- Ornamentate Decoration Material Wallpaper]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1)Author for correspondence