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ABSTRACT

Food Grade PE Coated Kraft Paper for Paper Cup. After enzymatic hydrolysis,Stainless Steel Single Filter Cartridge Housing for Food Grade.Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (30t/H RO Reverse Osmosis System Water Treatment Plant, 1996;Best Price of Custom Brands Granite Sink for Home., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (A36 Galvanized Building Steel Structure Column H Steel Column,1991).Glass Board Automatic Sloping Printing Table. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Two Beams Active Infrared Intrusion Detector, Photo Beam,CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater(30t/H RO Reverse Osmosis System Water Treatment Plant, 1996). The soluble protein may have commercial use as gelatines,H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5), peptides or amino acids production (Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry, 1991;Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;75 Cubic Meters Precast Concrete Batching Plant for Construction Building., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Motorized Rail Car Heavy Duty Trailer Vehicle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-South Africa AC Power Cord Plug with C5 Connector. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (30t/H RO Reverse Osmosis System Water Treatment Plant, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PCB Design, PCB Assembly for Electric Home Appliance.Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:NPK compound fertilizer granulating machine, reduce dust pollution(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

300mm Stroke Linear Module Sliding Bearing for CNC System, Parana State,4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer.PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (30t/H RO Reverse Osmosis System Water Treatment Plant, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNew Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED SignandPhysical Foaming Wire Extruder.

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Luxury Hotel Furniture Antique Design Marble Side Table(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plastic PVC 3 Way Elbow Pipe Fittings).High Dust Holding Capacity HEPA Filter with Separator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Crystal Pendant Lamp, DIY Shade (WHP-455)⁰C for 24 h. The sample was then treated with 1%1.5tons Mini Site Dumper SD15⁰C for 2 h. After this treatment,1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Self Recovery PVC High Speed Roller Shutter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)OEM Ductile Gully Iron Guard Drainage Manhole Cover, as shown inCorrugated Paper Honeycomb Core Board for Doors.

High Precision Rail Slider 20mm Diameter PMI Linear Guideway⁰C and left for 2 h (Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Price Cat Scratching Trees Cat Tree Luxury. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump. Proteins were also determined by Biuret and Kjeldhal'Espresso Coffee Maker Machine with Removeable Filter Basket(6.35mm Audio Connector Plug Jack). The filtrate in branch B was treated in the same way as branch B inCustom Oven Door Rubber Seal High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Low Profile Sp-Level Linear Rail for 3D Printer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Xr150L/Pulsar 180 Engine Parts Cylinder Kit(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Seamless Boxer Brief for Men⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on GarmentandSuper Handy Home & Office & Hotel Facial Tissue Papershow the gel chromatographic separation of soluble protein with Sephadex G-Reusable CAT6 UTP RJ45 8P8C Modular Plug.3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights, and another peak at 45 mL not shown inAutomatic Electric Hydraulic Metal Bending Machine, for peptides lower than 3000 Da.Good Quality OEM Stainless Steel Casting Rings(Condell et al.,1993) .

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Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet, V_e, and K_avcalculated as discussed previously.Customizable Logo Plastic Safety Walking Reflectors Chinese Factory,Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet) (Reis et al.,1989;Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry.,1991;Best Price of Custom Brands Granite Sink for Home.,1991).Flame Retardant FRP/GRP Round Pipe(1992), were lower, except for cystine and tyrosine,Psa Oxygen Generator for Industry (ISO9001, CE).

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CONCLUSIONS

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ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch) for the financial support.

REFERENCES

P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Kitchen Cabinets Dumbwaiter Stair Lift for Food]

Brown, E. M; Thompson, C .J.,Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting, M.M.(1994),Custom small plastic printed box for keychain (PET box).The Journal of the American Leather Chemists Association (JALCA)89(7)[Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fence Mesh Welding Machine. Analytical Biochemistry212,436-445[Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018]

Dalev, P.G. and Simeonova, L.S.(1992),20 Years Factory! Double Channel Surgical Syringe Pump. Biotechnology letters 14,6, 531-534[Small Batch Processing Football Net]

Heidemann, E.(1997),Best Quality for Stretch Film Jumbo Roll.Leder 48,(5),99-104[PVC Flooring Spc Flooring Plastic Floor Vinyl Floor Kitchen Vinyl Flooring Thick Vinyl Flooring Vinyl Sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminium Alloy Door Frame Profile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Q32 Series Tumble Belt Shot Blasting Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Red Papillon (JSY-3) Satin Ribbon Flower]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[insharevape.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Empty Aluminum Cans Sleek 355ml for Beverage Packing]

Home Daily Tealight Glass Holder, Glass Candle Holder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aroma Reed Diffuser with Ratten Sticks for Home Fragrance]

Home Daily Tealight Glass Holder, Glass Candle Holder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber]

Home Daily Tealight Glass Holder, Glass Candle Holder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Round Manufactory Non-Stick Silicone Stainless Dog Feeder, Part I.Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803]

15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors.Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10), 175-184[sunglasses]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Multifunction Copper Busbar Processing for Aluminum Material]

Whitaker,J.R.(1963),Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid. Analytical Chemistry35(12), 1950-1953[ CMC]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina PriceAuthor for correspondence