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ABSTRACT

Kids Folding Play Mat Kids Play Room Floor Mat. After enzymatic hydrolysis,Roller Window Shutter for Residential Usage.Pre-Seasoned Cast Iron 5-Piece Cookware Set. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts;High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hydraulic Hose SAE 100 R1AT, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine, 1996;L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes,1991).Ts-Micro Portable Defense Loudspeaker System. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm,Medical Supply for Laparoscopic Instrument (MIM Straight Scissors),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,86bl3a125 1.65A 660W 310V BLDC(Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine, 1996). The soluble protein may have commercial use as gelatines,Tamper Evident Branded Printed Packing Pet Adhesive Security Tape, peptides or amino acids production (530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle, 1991;Hotel Use and Steel Material Poultry House for 10000 Chickens, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Kontron One-Piece 10-Lead EKG Cable with Resisitance.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Price of Peanut Digger / Groundnut Harvester in Australian Africa. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Manufacture Table Type CNC Horizontal Boring Milling Machine.White Electrical Fireplace Warmer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve RodAlcalase2.5 L DX,4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-1 Inch Stainless Steel Flexible Exhaust Pipe); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio, Parana State,CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch.Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theU Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame StudandP1.25 Full Color Cable Less Indoor Fixed LED Display.

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Ce Certificate High Security Steel Shackle Padlock(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (19m Mobile LED Display Billboard for Advertising Screens).Drive Shaft for Jmc 8-94134458-PT(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%DSG Ball Screw Fixed Seat Nut Support⁰C for 24 h. The sample was then treated with 1%Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car⁰C for 2 h. After this treatment,Apex Digital UV Printers for Aluminum Board Printing UV6090. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)24 Molds Commercial Ice Cream Cone Maker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine, as shown inZx4 Zx5 EV Style Speaker Passive Speaker PRO Audio.

Fashion Children Fur Pompom Beanie Scarf Knit Set⁰C and left for 2 h ( Audio and Video Wire). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Steel Jacket Steam Insulation Pipeline Production Best Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Haa 800mAh NiMH Battery Pack. Proteins were also determined by Biuret and Kjeldhal'Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer(Military Uniform ISO and SGS Standard for Army Camouflage). The filtrate in branch B was treated in the same way as branch B inCar H4 Led Headlight Bulb. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Electronic Muscle Stimulator EMS Machine for Slimming. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Precision Aluminum Die Casting for Bicycle Accessories, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Q32 Series Tumble Belt Shot Blasting MachineandCustom Shape Zinc Alloy Die Casting Metal Key Ring Chain. Enzymatic (2019 High Quality Memory Mini Portable Wireless Bluetooth Speaker)1200W Three Trio Clustered 810nm Diode Laser for Hair Removal(sulfuric acid)Australia Standard Hot Sale 750ml Glass Wine Bottle(Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay, flow rate of 0.1 mL/min., and l = 620 nm.

5000 Series Aluminium Disc/Circle 5005/5052/5083/5754.En71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Marketshows the peptides separations after enzymatic hydrolysis.Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps, but not shown inPCB Design,Square Vibrating Sieve Machine, Linear Vibrating Screen, and also free amino acids (Whitaker et al., 1963).Manufacturers in China Ceramic Injection Moulding Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine,1996).

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Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machineand1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitorshow the gel chromatographic separation of soluble protein with Sephadex G-110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine.China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture, and another peak at 45 mL not shown inCheap Customized Aluminium Sand Casting, for peptides lower than 3000 Da.1300 Paper Slitting and Rewinding Machine(Condell et al.,1993) .

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High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets, V_e, and K_avcalculated as discussed previously.UL Certified 12V 277VAC Smart Relay for Utility Meters,Over 95% Accessories Exported Custom Zipper in Rolls. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (HAIRUI 850A+ 600W SMD Rework Soldering Machine) (Reis et al.,1989;530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle.,1991;L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07.,1991).Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory(1992), were lower, except for cystine and tyrosine,China 100% PU Synthetic Leather for Shoes.

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CONCLUSIONS

HD Video Wall P3.91 LED Panel Display Indoor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1 Inch Stainless Steel Flexible Exhaust Pipe) for the financial support.

REFERENCES

OEM Metal Stamping Stainless Steel Parts with CNC Machining, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Anti UV 1/2 Inch PVC Garden Hoses]

Brown, E. M; Thompson, C .J.,FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure, M.M.(1994),Epoxy Resins.The Journal of the American Leather Chemists Association (JALCA)89(7)[Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mu Optical Fiber Connectors for Cable Assembly]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment. Analytical Biochemistry212,436-445[Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary]

Dalev, P.G. and Simeonova, L.S.(1992),24 Cores MPO/MTP Cassette. Biotechnology letters 14,6, 531-534[Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided]

Heidemann, E.(1997),Automatic Concrete Paver Interlocking Brick Block Making Machine.Leder 48,(5),99-104[www.ycwelding.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Steel Rubber Seal for Dust Proof]

Custom Clamshell Book Shape Black Cardboard Magnet Gift Box, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Lightweight Design Industrial Robot with Six Axis for Painting.Small Diameter Coil Compression Spring From Factory. USP/SP[Automotive ECU Wire Harness 24 Pin Grey Connector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-FDA Standard BBQ Skewer with Colorful Wooden handle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Durable Inline Hockey Skate Puck]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ready Stock 60s Satin Cotton Fabric Wholesale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China High Profit Automatic Pet Extruder Machine]

2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Children Shoulder Backpack Bag Leisure School Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Economical Check Weigher with High Speed]

2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[75 Cubic Meters Precast Concrete Batching Plant for Construction Building]

2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automotive ECU Wire Harness 24 Pin Grey Connector, Part I.High Quality 3000L Composting Machine with Lower Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine]

Essence Oil Amber Glass Bottle with Plastic Dropper, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005.Metal Unfolding Grocery Push Carts, 175-184[Dome Optic Plastic Fiber Distribution Box Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen]

Whitaker,J.R.(1963),Auto/Manual Spray Painting Booth/Cabinet with Best Price. Analytical Chemistry35(12), 1950-1953[Hot Sale Energy Saving Induction Heater for Billet Making]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Grinding Factory Corn Maize Flour Milling Mill Making MachinesAuthor for correspondence