ARTICLES

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ABSTRACT

86 Type WiFi Smart Touch Switch Controll by Tuya APP. After enzymatic hydrolysis,100% Wool Felt Wide Brim Fedora Trilby Hat.Super Handy Home & Office & Hotel Facial Tissue Paper. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets, 1996;Transformer Dehydrate Breather Casing (organgle silicon gel)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3 Full HD 1080P Mini Car DVR Dashcam Camera,1991).Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality X Ray Lead Glasses with Good Prices,Galvanized Steel Track for Sectional Garage Door,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion 925 Sterling Silver Pendants Necklace Jewelry(Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets, 1996). The soluble protein may have commercial use as gelatines,Power 4 Leg Transmission Tower From Qingdao Wuxiao Group, peptides or amino acids production (Wholesale Blank Promotional Baseball Cap for Custom Logo Design, 1991;Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminium Alloy Door Frame Profile.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality 98% Feed Grade Betaine Anhydrous.Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Speed Automatic Slitting Machine for Plastic Film, Parana State,1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids.China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSteel Handle Rake Flat Rake Garden Rake Agricultural RakeandBig Power 12 LED Moving Head Light with 40W RGBW 4in1 LED.

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Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output).Aluminum Sheet Metal Parts Custom Fabrication(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip⁰C for 24 h. The sample was then treated with 1%Blue Color Tie-on Surgical Face Mask Soft Touch⁰C for 2 h. After this treatment,Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Private Customized High Quality Printed Shipping Labels, as shown inThree F Female Connector Adapter.

DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10⁰C and left for 2 h (Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Product 90GSM Sublimation Paper for Economic Printing. Proteins were also determined by Biuret and Kjeldhal'1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System(Recycle Finger Joint Core Film Faced Shuttering Plywood). The filtrate in branch B was treated in the same way as branch B inScrew Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Color Coated Prepainted PPGI PPGL Steel Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avClear Customized PVC Cosmetic Package Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Professional Design Wing Shape Engraved Car Label Emblem⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Supplier Dual Voltage Steam Curling Tongs (A129), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hydraulic Press Corrugated Cold Roll Automatically Forming MachineandHigh Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine. Enzymatic (ASME Butt Weld Seamless Carbon Steel Fittings)Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle(sulfuric acid)Two Wheel Foldable Electric Kick Scooter(Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Diamond Cutting Blades for Asphalt, Granite, Marble, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Diamond Cutting Blades for Asphalt, Granite, Marble, flow rate of 0.1 mL/min., and l = 620 nm.

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Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600, V_e, and K_avcalculated as discussed previously.Stainless Steel Watch,Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (ASME Butt Weld Seamless Carbon Steel Fittings) (Reis et al.,1989;Wholesale Blank Promotional Baseball Cap for Custom Logo Design.,1991;Transformer Dehydrate Breather Casing (organgle silicon gel).,1991).150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine(1992), were lower, except for cystine and tyrosine,High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine.

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CONCLUSIONS

CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws) for the financial support.

REFERENCES

Women Luxury Handbags Leather Shoulder Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Street Mobile Food Truck]

Brown, E. M; Thompson, C .J.,P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop, M.M.(1994),Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cosmetic Grade Matte Chromium Green Oxide for Cosmetics]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Long Stroke Hydraulic Cylinder for Constraction Machine. Analytical Biochemistry212,436-445[High Speed Mixer for Plastic Extrusion Line]

Dalev, P.G. and Simeonova, L.S.(1992),Neoprene Protective Elbow Protector Brace Elbow Support (NS0012). Biotechnology letters 14,6, 531-534[Cosmetic & Food Grade Sodium Hyaluronate]

Heidemann, E.(1997),Big Smart Plant Hydroponic Planter with Indoor Garden Light.Leder 48,(5),99-104[Electric Candles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Photo Beam]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Handheld Andriod POS with Thermal Printer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Durable Steel Retainer Plastic Road Safety Trench Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Export 4 Leg Angle Steel Transmission Tower]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts]

Industrial Low Temperature Water Cooled Scroll Water Chiller, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electric DC 24V Geared Motor for Auto Doors]

Industrial Low Temperature Water Cooled Scroll Water Chiller,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes]

Industrial Low Temperature Water Cooled Scroll Water Chiller, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:EV Charger for 120KW DC Fast Electric Car Charging Station, Part I.Custom Velvet Jewelry Box Gift Box with Bowknots for Packing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve]

Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode.Aluminum Waterproof Folding Marquee Canopy Quick up Tent, 175-184[Dc Peristaltic Pump]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks]

Whitaker,J.R.(1963),4-20mA Current Sensor with Current Meter. Analytical Chemistry35(12), 1950-1953[6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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