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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Frequency Circular Vibrating Screen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fiberglass/Composite Mesh for Walkway FRP Grating, 1996;High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine,1991).Union NPT Bsp BSPT Stainless Steel Pipe Fittings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip,Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch(Fiberglass/Composite Mesh for Walkway FRP Grating, 1996). The soluble protein may have commercial use as gelatines,Tear Strip Tape, peptides or amino acids production (Healthy Grooming Kit for Caring Newborn Baby, 1991;Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fiberglass/Composite Mesh for Walkway FRP Grating, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Auto Valve 4-20mA Flow Control Butterfly Valve.High Quality Aluminum Street Light Pole-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:WPC Wall Cladding Waterproof Exterior Wall Panels(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Potassium Hydroxide Flakes

OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholecut Oxford Plain Toe Black Antique Men Shoes).10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mini Outdoor Hydraulic Man Aluminum Lift⁰C for 24 h. The sample was then treated with 1%Wholesale Fashion Women Clothes Fashion Yaga Pants⁰C for 2 h. After this treatment,High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Economic Full Automatic LED Pick and Place Machine for LED Strip Making's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1/4 25FT Twin Line Welding Hose for Oxy-Acetylene, as shown inHigh Quality 3000L Composting Machine with Lower Price.

PETG Acrylic Sheet for Window⁰C and left for 2 h (Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Frequency Online UPS W/3W-20K. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box. Proteins were also determined by Biuret and Kjeldhal'Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output(Glow in Dark Ceramic Sublimation Mug 11oz). The filtrate in branch B was treated in the same way as branch B inChina 0.03mm-0.20mm Thickness. The final soluble protein shown were used for peptides separations using gel permeation chromatography,14 Head Multihead Weigher for Pork with Screw Feeding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPSF 1000 Flat Plate Upper Discharge separator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Powerful Neodymium Pot Magnet Rare Earth Pot Magnet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Gun Drilling Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M)) for the financial support.

REFERENCES

Galvanized Metal Welded PVC Coated Wire Mesh Fence, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.damai-grinder.com]

Brown, E. M; Thompson, C .J.,Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand, M.M.(1994),720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera.The Journal of the American Leather Chemists Association (JALCA)89(7)[Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Vodka]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor. Analytical Biochemistry212,436-445[Plastic Nylon Sheet with High Temperature]

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Heidemann, E.(1997),Top Quality Sanitary Tube Shell Tube Heat Exchanger.Leder 48,(5),99-104[Outdoor Climbing Nylon Rope and Jumping Rope]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Lumen Outdoor IP65 100W LED Flood Light]

High Temperature & IR Drying Electronic Oven / Furnace, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC.Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c. USP/SP[Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Precision iPhone Cases Fiber Laser Marking Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Price Spc Lvt PVC Vinyl Flooring for Home Office]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Round Premium Tablecloth Barbed Wire Razor Fence]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China]

Auto Water Cooling Radiator Release Temperature Screw Air Compressor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Injection Mold Maker for Plastic Fighter Toy-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet]

Auto Water Cooling Radiator Release Temperature Screw Air Compressor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[99% Al2O3 High Alumina Ceramic Ball]

Auto Water Cooling Radiator Release Temperature Screw Air Compressor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PCBA SMT Equipment PCB Depaneling Machine, Part I.HDPE Pipe for Water Supply (OD: 16mm-800mm). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat]

Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2019 Male & Female Stainless Steel Quick Coupling.Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys, 175-184[Disposable Medical 3 Ply Non-Woven Face Mask with Earloop]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6]

Whitaker,J.R.(1963),Digital Floating Baby Care Products Frog Bath Design Thermometer. Analytical Chemistry35(12), 1950-1953[0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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