ARTICLES

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^IICNPq/RHAE/biotechnology-New Design Slotted Board MDF for Wall Panel. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Factory Wholesale Reach Standard Home and Office Furniture Fitting

ABSTRACT

Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing. After enzymatic hydrolysis,Hot Sale Energy Saving Induction Heater for Billet Making.Electric 154 Stone Polisher 17inch Floor Cleaning Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Pin Custom Stamping, Punch Punching Parts, Stamping Metal Parts, Brass Stamping, Electrical Stamping, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018, 1996;CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Portable Polyester Duffel Garment Tote Bag,1991).Decoration Sign Green Grey White PVC Foam Board. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3kw 4kw 5kw off Grid Solar Inverter,OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,A616 Axe with Wooden Handle Series(Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018, 1996). The soluble protein may have commercial use as gelatines,IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B, peptides or amino acids production (Pet Material Alien 9654 Wet Inlay UHF Windshield Label, 1991;Reinforced Brass Coated Steel Wire for Hose Wire, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.EPE Foam Package Extruding Production Line.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3 Layer Film Blowing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰8FT Wood Log Debarker Machine for Plywood Machinery.Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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6.35mm Audio Connector Plug Jack⁰C and left for 2 h (Hidden Handle Safe for Children in Bathroom Cabinet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62. Proteins were also determined by Biuret and Kjeldhal'Motar Plastering Machine with Screw Pump(CNC Engraving Machine Laser Marking for Assembly Line). The filtrate in branch B was treated in the same way as branch B inCustomized Sintered Smco Magnets Exporter Hot Sale Product. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFr4 Copper Clad Laminate PCB Manufacture Company(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ibutamoren Mesylate Mk-677 for Healing Injuries Sarms⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

78mm Aluminum Gold Color Plastic Jar Screw CapandAuto Ignition Coil. Buick, Chevrolet, Cruz, (with mold/without mold) , etc. Model: 55571790/55571790 B.. Enzymatic (Flip PU Tablet Case Cover for iPad iPad Mini 4 Cover)Custom Aluminum Alloy Die-Casting Housing for Bluetooth Speakers(sulfuric acid)50kVA Oil Immersed Transformer, 10kv 11kv Power Distribution Transformer Power Transformer(Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle, flow rate of 0.1 mL/min., and l = 620 nm.

Auto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11.Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Boardshows the peptides separations after enzymatic hydrolysis.Automatic Soap Bag in Box Packing Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Precision Plastic Mold Manufacturer in China, but not shown inGood Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337),ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting, and also free amino acids (Whitaker et al., 1963).High Performance Alcohol Pad Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018,1996).

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Hydraulic Presses

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Digital Control with LED Display DC Solar Small Deep Freezer Fridge, V_e, and K_avcalculated as discussed previously.Automatic Economical Check Weigher with High Speed,Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting) (Reis et al.,1989;Pet Material Alien 9654 Wet Inlay UHF Windshield Label.,1991;CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101.,1991).Steel Handle Rake Flat Rake Garden Rake Agricultural Rake(1992), were lower, except for cystine and tyrosine,High Quality Small Tea Bag Packing Machine Price.

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CONCLUSIONS

Carbon Dioxide Gas with 50L Seamless Cylinder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire, M.M.(1994),Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ball Mill]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Swbf 7b2s Bolt Former, Bolt Machine, Cold Forging Machine, Cold Heading Machine, Parts Former, Nut Tapping Machine, Threadrolling Machine, Automatic Bolt Making. Analytical Biochemistry212,436-445[Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Fashion Logo Sport Rubber Silicone Bracelet Customized Printed Smart RFID Watch USB Mosquito Imprinted Embossed Silicon Wristband for Promotional Gift. Biotechnology letters 14,6, 531-534[Lace Control Hiphugger Braless Latex Body Shaper L81187]

Heidemann, E.(1997),Smiley Canvas School Backpack for Teenagers Girls.Leder 48,(5),99-104[Crocodile Leather Hand Bag Supplier & Factory]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Surface Cotton]

2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen.Good Quality CZ Purlin One Machine Roll Forming Machine. USP/SP[Ge Holter Cable Snap Type Holter ECG Cable]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Supra Brand Laundry Liquid Baby Formula]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM Customized Logo Print Polyester Nylon Gym Sport Blue Drawstring Backpack Bag Supplier]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[GRP FRP Flat Rod Pultruded Strips Fiberglass]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Gold Open Metal Short Crocodile Wallet for Girl Women Lady]

Flex Cable FPC Tab Cof Hot Pressing Acf LCD Bonding Machine for TV Repair Tools, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs]

Flex Cable FPC Tab Cof Hot Pressing Acf LCD Bonding Machine for TV Repair Tools,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee]

Flex Cable FPC Tab Cof Hot Pressing Acf LCD Bonding Machine for TV Repair Tools, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box, Part I.Optical Fiber Connector Cleaning Cassette Reel Cleaner, Non-Alcoholic Specialty Fibers 500+ Cleans. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ce Certificate High Security Steel Shackle Padlock]

Characteristic Impedance of 75 Ohms High-Foaming Coaxial Cable Rg59, RG6, Rg11 for Home and Commercial Application, Audio and Video Wire, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Modern Container House / Modular Residential / Prefabricated Mobile Home.100% Polyester Luxury Bedding Comforter Fleece Blanket Throw, 175-184[Winter Shoes Used Boot Men′s Leather Shoes]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Blue Color Tie-on Surgical Face Mask Soft Touch]

Whitaker,J.R.(1963),Aluminum Window System Thermal Break Strip (HK Stype35.3mm). Analytical Chemistry35(12), 1950-1953[Nylon Hammer Fixings Drive Anchor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding ApplicationsAuthor for correspondence