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ABSTRACT

Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet. After enzymatic hydrolysis,3phase DC to AC Variable Frequency Drive Water Solar PV Inverter.Quality Printed Cigarette Tipping Paper. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;White Latex Paper Yellow/White Stearate Coated Abrasive Paper;Manufacturer Automotive CNC Machined Parts Checking Fixture Components; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Telescopic Slideway Steel Protection Accordion Cover, 1996;Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cosmetic Grade Matte Chromium Green Oxide for Cosmetics,1991).Ridge Cap Panel Forming Machine Ridge Cap Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Grinding Factory Corn Maize Flour Milling Mill Making Machines,Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Construction & Decoration ERW Square Steel Tube Manufacturer(Telescopic Slideway Steel Protection Accordion Cover, 1996). The soluble protein may have commercial use as gelatines,Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet , peptides or amino acids production (Household Use Food Packing 8011 Aluminum Foil, 1991;Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic Case Navigation System Tooling Injection Mold.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Telescopic Slideway Steel Protection Accordion Cover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰8X8mm Silver Expanded Metal Mesh in Hot Sale.Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:100% Durable Flooring Roll for Hotel Office School and Health Project Floor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Sc/APC Fiber Optic Fast Hot Melt Connector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay).Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates⁰C for 24 h. The sample was then treated with 1%Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)⁰C for 2 h. After this treatment,Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, as shown inHigh Precision iPhone Cases Fiber Laser Marking Machine.

Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006⁰C and left for 2 h (Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer. Proteins were also determined by Biuret and Kjeldhal'Food Grade PE Coated Kraft Paper for Paper Cup(DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin). The filtrate in branch B was treated in the same way as branch B inLight Weight Roller Shutters. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Single Filter Cartridge Housing for Food Grade.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM Stainless Steel Investment Casting Bearing for Car Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/30t/H RO Reverse Osmosis System Water Treatment Plant, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Price of Custom Brands Granite Sink for Home, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5).Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machineshows the peptides separations after enzymatic hydrolysis.Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inModern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric, but not shown inNail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply,Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts, and also free amino acids (Whitaker et al., 1963).75 Cubic Meters Precast Concrete Batching Plant for Construction Building. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Telescopic Slideway Steel Protection Accordion Cover,1996).

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Spiral Rib DiesandHuman Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depressionshow the gel chromatographic separation of soluble protein with Sephadex G-Motorized Rail Car Heavy Duty Trailer Vehicle.South Africa AC Power Cord Plug with C5 Connector, and another peak at 45 mL not shown inLayflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch, for peptides lower than 3000 Da.PCB Design, PCB Assembly for Electric Home Appliance(Condell et al.,1993) .

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CONCLUSIONS

Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale) for the financial support.

REFERENCES

300mm Stroke Linear Module Sliding Bearing for CNC System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Beach Shorts]

Brown, E. M; Thompson, C .J.,PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB, M.M.(1994),Luxury Hotel Furniture Antique Design Marble Side Table.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Model Water Source Heat Pump (SLW100D)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Dust Holding Capacity HEPA Filter with Separator. Analytical Biochemistry212,436-445[Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags]

Dalev, P.G. and Simeonova, L.S.(1992),Crystal Pendant Lamp, DIY Shade (WHP-455). Biotechnology letters 14,6, 531-534[Manual Glass Lifting Machine for Making Double Glass]

Heidemann, E.(1997),1.5tons Mini Site Dumper SD15.Leder 48,(5),99-104[Diploma Cover]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand]

Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube.Stainless Steel Self Recovery PVC High Speed Roller Shutter. USP/SP[High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Men Genuine Leather Sling Shoulder Satchel Messenger Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Ductile Gully Iron Guard Drainage Manhole Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Community IC Access Card Induction ID Time Card Smart Card]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Women Sneakers/Trainers]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Waste Paper Stripper]

High Precision Rail Slider 20mm Diameter PMI Linear Guideway, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Price Cat Scratching Trees Cat Tree Luxury-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag]

High Precision Rail Slider 20mm Diameter PMI Linear Guideway,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Extruder Floating/Sinking Fish Feed Pellet Dryer]

High Precision Rail Slider 20mm Diameter PMI Linear Guideway, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump, Part I.Espresso Coffee Maker Machine with Removeable Filter Basket. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line]

Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Low Profile Sp-Level Linear Rail for 3D Printer.China Xr150L/Pulsar 180 Engine Parts Cylinder Kit, 175-184[Perfect White Beauty Collagen]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine]

Whitaker,J.R.(1963),High Quality Paulownia Wood Board Wood Drying Kilns for Sale. Analytical Chemistry35(12), 1950-1953[Manufacturers in China Ceramic Injection Moulding Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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