ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-PVC Shrink Sleeve Bottle Label for Can Packing. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable

ABSTRACT

Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch. After enzymatic hydrolysis,Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2.300mm Stroke Linear Module Sliding Bearing for CNC System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB;Luxury Hotel Furniture Antique Design Marble Side Table; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Dust Holding Capacity HEPA Filter with Separator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Crystal Pendant Lamp, DIY Shade (WHP-455), 1996;1.5tons Mini Site Dumper SD15., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube,1991).Stainless Steel Self Recovery PVC High Speed Roller Shutter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,OEM Ductile Gully Iron Guard Drainage Manhole Cover,High Precision Rail Slider 20mm Diameter PMI Linear Guideway,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Price Cat Scratching Trees Cat Tree Luxury(Crystal Pendant Lamp, DIY Shade (WHP-455), 1996). The soluble protein may have commercial use as gelatines,China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump, peptides or amino acids production (Espresso Coffee Maker Machine with Removeable Filter Basket, 1991;Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Low Profile Sp-Level Linear Rail for 3D Printer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Xr150L/Pulsar 180 Engine Parts Cylinder Kit.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Paulownia Wood Board Wood Drying Kilns for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Crystal Pendant Lamp, DIY Shade (WHP-455), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Seamless Boxer Brief for Men.Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

100ml Square Glass Diffuser Bottle with Cap, Parana State,OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable.Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Crystal Pendant Lamp, DIY Shade (WHP-455), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGood Quanlity of Ue319an20z Hydraulic Oil Filter ElementandHigh Pressure Low Volume 2900rpm electric Water Pump.

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Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cosmetic & Personal Care Grade Xanthan Gum).Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS⁰C for 24 h. The sample was then treated with 1%Reusable CAT6 UTP RJ45 8P8C Modular Plug⁰C for 2 h. After this treatment,3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Good Quality OEM Stainless Steel Casting Rings's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet, as shown inCNC Machinery Aluminum and ABS Mock up Prototypes.

Customizable Logo Plastic Safety Walking Reflectors Chinese Factory⁰C and left for 2 h (Thick Wall Large Diameter Aluminium Tube Manufacture). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Flame Retardant FRP/GRP Round Pipe. Proteins were also determined by Biuret and Kjeldhal'Psa Oxygen Generator for Industry (ISO9001, CE)(Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)). The filtrate in branch B was treated in the same way as branch B inUs Smart Wall Touch Switch 110~220V RF WiFi Switch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAirtac Tubing Quick Connect Puick Coupling Pneumatic Fitting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fence Mesh Welding Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/20 Years Factory! Double Channel Surgical Syringe Pump, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Quality for Stretch Film Jumbo Roll, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Full Automatic Combination Weighing Packing Machine Jy-420A. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (150ml Round Aromatherapy Diffuser Glass Bottle) for the financial support.

REFERENCES

Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory Fashion Design Wholesale Deluxe OEM Pet House Cat Climbing Tree]

Brown, E. M; Thompson, C .J.,Tfl Blue Rubber Transmission Belts/Automotive Spare Part, M.M.(1994),1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China.The Journal of the American Leather Chemists Association (JALCA)89(7)[High End L Shape Modular Sectional Sofa Patio Furniture Set Garden]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Flat Wedge Wire Panel China Factory. Analytical Biochemistry212,436-445[Diamond Cutting Blades for Asphalt]

Dalev, P.G. and Simeonova, L.S.(1992),Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625. Biotechnology letters 14,6, 531-534[Wholecut Oxford Plain Toe Black Antique Men Shoes]

Heidemann, E.(1997),Best Selling Hydraulic Small Truck with Crane 10 Ton.Leder 48,(5),99-104[Punch Breathable Neoprene Patella Strap Knee Support]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality 3000L Composting Machine with Lower Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Glow in Dark Ceramic Sublimation Mug 11oz]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Design Disposable Trocars for Laparoscopic Surgery: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[printing Rehabilitation of protective]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[52cc Petrol Brush Cutter for DIY Garden Care]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight]

Disposable Medical Combined Bacterial Viral Hme Filter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control]

Disposable Medical Combined Bacterial Viral Hme Filter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Children Fur Pompom Beanie Scarf Knit Set]

Disposable Medical Combined Bacterial Viral Hme Filter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System, Part I.3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[K-Pop Bts Baseball Hat Sport Anime Cap]

High Efficiency Pineapple Canning Plant, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Embossed Pattern PU Leather Classic Mens Wallet.Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles, 175-184[Cement And Sand Screed]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pet Material Alien 9654 Wet Inlay UHF Windshield Label]

Whitaker,J.R.(1963),Casting PE Film with Ash for Adult Diaper Back Sheet. Analytical Chemistry35(12), 1950-1953[Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

E2000/APC Fibre Optic Leads and CablesAuthor for correspondence