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ABSTRACT

Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M). After enzymatic hydrolysis,Stainless Steel LED Underground Light, Ground Lighting IP66.GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mini Outdoor Hydraulic Man Aluminum Lift, 1996;Wholesale Fashion Women Clothes Fashion Yaga Pants., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride,1991).Economic Full Automatic LED Pick and Place Machine for LED Strip Making. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1/4 25FT Twin Line Welding Hose for Oxy-Acetylene,PETG Acrylic Sheet for Window,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Frequency Online UPS W/3W-20K(Mini Outdoor Hydraulic Man Aluminum Lift, 1996). The soluble protein may have commercial use as gelatines,Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box, peptides or amino acids production (Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output, 1991;14 Head Multihead Weigher for Pork with Screw Feeding, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PSF 1000 Flat Plate Upper Discharge separator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mini Outdoor Hydraulic Man Aluminum Lift, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Powerful Neodymium Pot Magnet Rare Earth Pot Magnet.CNC Gun Drilling Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

High Whiteness & Excellent Dispersity Anatase Type Titanium DioxideAlcalase2.5 L DX,Large Jewelry Display Box for Ring Necklace Earring Storage Case, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Indoor Tub Customized Shower/Free Standing Shower Enclosure); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Glow in Dark Ceramic Sublimation Mug 11oz(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker, Parana State,Tall Thin Lowes Wood Storage Cabinet (GSP20-008).Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Mini Outdoor Hydraulic Man Aluminum Lift, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFiberglass/Composite Mesh for Walkway FRP GratingandNon - Smell Fiber Reinforced PVC Garden Hose.

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22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hydraulic Hose SAE 100 R1AT).Supermarket Combined Island Chest Freezer Commercial Refrigerator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price⁰C for 24 h. The sample was then treated with 1%High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair⁰C for 2 h. After this treatment,Clip on Wire Shelving Data Strip. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Black and White Hard Laptop Case Travel Suitcase's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Concrete Road Crack-Pouring Machine for Construction, as shown inProfessional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service.

Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck⁰C and left for 2 h (Metal Tin with Clear Lid). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China OEM Alloy Steel Precision Casting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport. Proteins were also determined by Biuret and Kjeldhal'China Best Quality Hose Crimping Machine(Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle). The filtrate in branch B was treated in the same way as branch B incoffee table manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Galvanized Metal Welded PVC Coated Wire Mesh Fence. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avButyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Top Quality Sanitary Tube Shell Tube Heat Exchanger, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cameleon Color Changing Car Paint Chameleon Pigment for Auto PaintandDifferent Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC. Enzymatic (Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c)High Precision iPhone Cases Fiber Laser Marking Machine(sulfuric acid)Auto Water Cooling Radiator Release Temperature Screw Air Compressor(Mini Outdoor Hydraulic Man Aluminum Lift, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Injection Mold Maker for Plastic Fighter Toy, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Injection Mold Maker for Plastic Fighter Toy, flow rate of 0.1 mL/min., and l = 620 nm.

PCBA SMT Equipment PCB Depaneling Machine.Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Headshows the peptides separations after enzymatic hydrolysis.HDPE Pipe for Water Supply (OD: 16mm-800mm)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOrange Silica Gel 2-4 mm with High Quality, but not shown inNichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip,Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, and also free amino acids (Whitaker et al., 1963).2019 Male & Female Stainless Steel Quick Coupling. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Mini Outdoor Hydraulic Man Aluminum Lift,1996).

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Dongfeng 4X2 10000L Water Spinkling Tank Transport TruckandGalvanized Steel Track for Sectional Garage Doorshow the gel chromatographic separation of soluble protein with Sephadex G-Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys.Digital Floating Baby Care Products Frog Bath Design Thermometer, and another peak at 45 mL not shown inFireproof WPC Indoor Decorative Wall Panel, for peptides lower than 3000 Da.Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine(Condell et al.,1993) .

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CONCLUSIONS

18mm Thickness Best Quality Poplar Birch Commercial Plywood. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Indoor Tub Customized Shower/Free Standing Shower Enclosure) for the financial support.

REFERENCES

European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Geared Elevator Traction Machine Dimension Parameter Yj245 Passenger Lift]

Brown, E. M; Thompson, C .J.,Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box, M.M.(1994),Nylon Hammer Fixings Drive Anchor.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 Dotted Navy Rucksack Children School Bag Cotton Backpack]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[8X8mm Silver Expanded Metal Mesh in Hot Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM Electrolyte Effervescent Tablet. Analytical Biochemistry212,436-445[Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh. Biotechnology letters 14,6, 531-534[Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery]

Heidemann, E.(1997),6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter.Leder 48,(5),99-104[1.2m Automatic LED Visual Pick and Place Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Over Band Round Type Electro Magnetic Separator for Tramp Iron]

WPC Wall Cladding Waterproof Exterior Wall Panels, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet.20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant. USP/SP[South Africa AC Power Cord Plug with C5 Connector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Precision Mini Laser Cutting System]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-304 321 310 310S Welded Decorative Stainless Steel Pipe: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Cycling Rack Home Floor Bike Parking Rack for 4 Bikes]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Smiley Canvas School Backpack for Teenagers Girls]

Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Manual Electric Winch Small Winch 220V Winch-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Canvas Shoulder Weekend Shopping Big Bag]

Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Liquid Hardener Setting Cement Membrane Curing Concrete]

Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price, Part I.Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Large Jewelry Display Box for Ring Necklace Earring Storage Case]

Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood.Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part, 175-184[Powder or Granule Packaging Machinery]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5]

Whitaker,J.R.(1963),Aluminum Plate Fin Heat Exchanger/Oil Cooler Core. Analytical Chemistry35(12), 1950-1953[High Grade Nitrous Oxide Gas / Laughing Gas / N2o]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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