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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Kraft Paper Laminated Aluminum Foil. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800

ABSTRACT

Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600. After enzymatic hydrolysis,Stainless Steel Watch.Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine;High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Women Luxury Handbags Leather Shoulder Bag, 1996;P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel,1991).Long Stroke Hydraulic Cylinder for Constraction Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Neoprene Protective Elbow Protector Brace Elbow Support (NS0012),Big Smart Plant Hydroponic Planter with Indoor Garden Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric(Women Luxury Handbags Leather Shoulder Bag, 1996). The soluble protein may have commercial use as gelatines,High Quality Anti-Corrosion Fiberglass Sheet and Roll, peptides or amino acids production (Durable Steel Retainer Plastic Road Safety Trench Cover, 1991;Industrial Low Temperature Water Cooled Scroll Water Chiller, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.EV Charger for 120KW DC Fast Electric Car Charging Station.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Velvet Jewelry Box Gift Box with Bowknots for Packing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Women Luxury Handbags Leather Shoulder Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush.Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminum Waterproof Folding Marquee Canopy Quick up Tent(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality LED Wall Mirror with Ce, UL, cUL Certificate(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (150 Ton Hydraulic Press Machine for Cooking Pot Making).Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag⁰C for 24 h. The sample was then treated with 1%The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel⁰C for 2 h. After this treatment,Die-Casting Aluminium Radiator (RN808-500). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholecut Oxford Plain Toe Black Antique Men Shoes, as shown inEaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts.

New Technology Carbon Cleaning Tools Auto Accessories⁰C and left for 2 h (SBR Round DOT Horse Stall Mat). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Environmental Test Salt Spray Tester Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom 2 Ring Binder PP Lever Arch Clip File Folder. Proteins were also determined by Biuret and Kjeldhal'Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant(China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile). The filtrate in branch B was treated in the same way as branch B inMetal Cable Clamp. The final soluble protein shown were used for peptides separations using gel permeation chromatography,TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Laser Customized Telescopic Steel Cover. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Precision Mini Laser Cutting System(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/One to One Order Following Strong Real Gold Thread, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Design Paper Pyramid Memo Cube with Ribbon for Promotion, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Light Weight Ohsas 18001 Certified Metal Arch Buildings) for the financial support.

REFERENCES

Telescopic Slideway Steel Protection Accordion Cover, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Coffee Vending Trailer For Sale]

Brown, E. M; Thompson, C .J.,Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes, M.M.(1994),Cosmetic Grade Matte Chromium Green Oxide for Cosmetics.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Gas Spring Strut Gas Shock for Wall Bed]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ridge Cap Panel Forming Machine Ridge Cap Making Machine. Analytical Biochemistry212,436-445[100% Wool Felt Wide Brim Fedora Trilby Hat]

Dalev, P.G. and Simeonova, L.S.(1992),Grinding Factory Corn Maize Flour Milling Mill Making Machines. Biotechnology letters 14,6, 531-534[New Fashion TPU Mobile Cover Case for Huawei]

Heidemann, E.(1997),Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male.Leder 48,(5),99-104[Hl-1016-5050A LED Strip Light with High Quality 5050LED Strip SMD LED Christmas Decoration Christmas Light LED Strip Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover]

Shoelace-Free Climbing Shoes with Wire Button, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Construction & Decoration ERW Square Steel Tube Manufacturer.Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet . USP/SP[High Quality Anti-Corrosion Fiberglass Sheet and Roll]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Round Manufactory Non-Stick Silicone Stainless Dog Feeder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Household Use Food Packing 8011 Aluminum Foil: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Super Soft 100% Viscose From Bamboo Bedding Set]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Interior Decorative Polyester Fiber 3D Acoustic Wall Panel / Ceiling Board]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High End Folding Customized Golden Chocolate Paper Box]

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Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI]

Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic Case Navigation System Tooling Injection Mold, Part I.Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Electric PCB Circuit Board with High Quality]

8X8mm Silver Expanded Metal Mesh in Hot Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst.100% Durable Flooring Roll for Hotel Office School and Health Project Floor, 175-184[Anodized Aluminum Sheet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry]

Whitaker,J.R.(1963),Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight. Analytical Chemistry35(12), 1950-1953[Bitumen Waterproof Membrane Minerla Surface Waterproof Material]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Centrifugal Casting Stainless Steel Furnace TubeAuthor for correspondence