ARTICLES

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ABSTRACT

Proper Price Good Quality 10ml 20ml Airless Pump Bottle. After enzymatic hydrolysis,BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener.Fire Retardant Reflective Aluminium Foil Woven for Insulation Material. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine, 1996;CNC Machinery Aluminum and ABS Mock up Prototypes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bsp 60° Male Cone Seat Carbon Steel Fittings,1991).Soccer Field Artificial Grass Cutting Machine for Installation. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Nylon Sheet with High Temperature,PVC TPU Tr 2 Color Sole Injection Moulding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors(High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine, 1996). The soluble protein may have commercial use as gelatines,Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc, peptides or amino acids production (Corrugated Paper Honeycomb Core Board for Doors, 1991;Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wood Handle Carbon Steel Sickle S202., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Custom Golden Colored Powder Pill Organizer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Disposable Pipette Tip 40~200UL with Ce.Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hidden Handle Safe for Children in Bathroom Cabinet, Parana State,Hexagonal Netting/ Gabion Box/ Gabion Basket.Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustom Makeup 10 Color Private Label Eyeshadow Case Palette PackagingandWholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet.

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Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve).Transformer Dehydrate Breather Casing (organgle silicon gel)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3 Full HD 1080P Mini Car DVR Dashcam Camera⁰C for 24 h. The sample was then treated with 1%Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank⁰C for 2 h. After this treatment,High Quality X Ray Lead Glasses with Good Prices. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Galvanized Steel Track for Sectional Garage Door's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashion 925 Sterling Silver Pendants Necklace Jewelry, as shown in4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes.

Power 4 Leg Transmission Tower From Qingdao Wuxiao Group⁰C and left for 2 h (Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Blank Promotional Baseball Cap for Custom Logo Design. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building. Proteins were also determined by Biuret and Kjeldhal'Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine(Delrin Sheets with Good Wear Resistance). The filtrate in branch B was treated in the same way as branch B inAir Filter 28113-3x000. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminium Alloy Door Frame Profile.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality 98% Feed Grade Betaine Anhydrous(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional Pulse Dust Filter for Grain Processing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plug-in Assembly Type Straight Through Joint and Insulation Joint, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Aluminum Sheet Metal Parts Custom Fabrication.3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulbshows the peptides separations after enzymatic hydrolysis.Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting, but not shown inSupermarket Combined Island Chest Freezer Commercial Refrigerator,Blue Color Tie-on Surgical Face Mask Soft Touch, and also free amino acids (Whitaker et al., 1963).Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine,1996).

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CL2T-SF304L Comlom Military Folding Steel Spade with Portable PouchandCummins L10 Engine Piston Cooling Nozzle 3070218show the gel chromatographic separation of soluble protein with Sephadex G-Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak.Private Customized High Quality Printed Shipping Labels, and another peak at 45 mL not shown inEurope Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet, for peptides lower than 3000 Da.DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10(Condell et al.,1993) .

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CONCLUSIONS

Clear Customized PVC Cosmetic Package Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Super Handy Home & Office & Hotel Facial Tissue Paper) for the financial support.

REFERENCES

Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Large Letter Stickers]

Brown, E. M; Thompson, C .J.,Factory Professional Design Wing Shape Engraved Car Label Emblem, M.M.(1994),38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Apex Digital UV Printers for Aluminum Board Printing UV6090]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Supplier Dual Voltage Steam Curling Tongs (A129). Analytical Biochemistry212,436-445[Auto Valve 4-20mA Flow Control Butterfly Valve]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine. Biotechnology letters 14,6, 531-534[Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS]

Heidemann, E.(1997),ASME Butt Weld Seamless Carbon Steel Fittings.Leder 48,(5),99-104[Clothing Hard Tag Security Tag for Supermarket/Retail Store]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport]

Universal Manual Turret Milling Machine(Mill X6336), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle.Two Wheel Foldable Electric Kick Scooter. USP/SP[5-Axis CNC Machining Billet Aluminum Engine Rotary Housing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Diamond Cutting Blades for Asphalt, Granite, Marble: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Calcium Chloride 94% Min]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine]

Best Selling Road Reflective Rectangle Guardrail Delineator Reflector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3-110W Loudspeaker Compression Driver, Neodymium Magnet Tweeter, Gt-7506na-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Afghanistan]

Best Selling Road Reflective Rectangle Guardrail Delineator Reflector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Price Long Life Portable Peltier Air Cooler]

Best Selling Road Reflective Rectangle Guardrail Delineator Reflector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PE Pipe in SDR 11 Pressing Rate for Fire-Control, Part I.Electric Automotive Heater Control Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross]

Shoelace-Free Climbing Shoes with Wire Button, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Kraft Paper Laminated Aluminum Foil.China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800, 175-184[Fashion Promotional Foldable Bag Shopping Bag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cavitation Vacuum RF Lipo Laser Slimming Machine]

Whitaker,J.R.(1963),Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600. Analytical Chemistry35(12), 1950-1953[Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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