ARTICLES

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ABSTRACT

Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625. After enzymatic hydrolysis,Best Selling Hydraulic Small Truck with Crane 10 Ton.Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Disposable Medical Combined Bacterial Viral Hme Filter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale, 1996;Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb,1991).High Efficiency Pineapple Canning Plant. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Embossed Pattern PU Leather Classic Mens Wallet,Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Casting PE Film with Ash for Adult Diaper Back Sheet(Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale, 1996). The soluble protein may have commercial use as gelatines,Supra Brand Laundry Liquid Baby Formula, peptides or amino acids production (Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger, 1991;Cleaning Products Sponge Vacuumized Pack 65mm, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mineral Water Bottle Mould Plastic Blowing Mold., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications.Cookies Cake Automatic Rotary Packing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Building Material Quick Install Types of Scaffolding Couplers(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Mini Succulent Planters, Cactus Pots with Wooden Cement Pot(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (6082 and 6061 Aluminum CNC Machining Parts for Aircraft).Folded Kitchen Towel Paper Wrapping Packaging Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cheap Topmac Brand Mobile Concrete Mixing Plant⁰C for 24 h. The sample was then treated with 1%Color Stretch Film for Pallet Wrap Use⁰C for 2 h. After this treatment,Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hemp Recycle Polyester Blenched Jersey Knit Fabric's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV, as shown in Suitable Apple iPhone and Android Charge.

Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule⁰C and left for 2 h (Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3D Creative Design Metal Medal Promotion Gift of Sport. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line. Proteins were also determined by Biuret and Kjeldhal'Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe(Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale). The filtrate in branch B was treated in the same way as branch B inToner Cartridge Chip for Xerox Phaser 7425 7428 7435 (006R01395 006R01396 006R01397 006R01398). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Composite Plate Dedicated Drop Hose Gear Metering Pump Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Brake Fluid Tank Container Hot Plate Plastic Welding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Puffed Core Filling Sanck Production Machine with Ce From China, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Electric 100W Three Wheels Toy Car for Sale.Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machineshows the peptides separations after enzymatic hydrolysis.New Design Customized 100% Cotton Home Towel Bath Towel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62, but not shown inAGM Separator for AGM Battery,Toughened Railing Glass, and also free amino acids (Whitaker et al., 1963).Delrin Sheets with Good Wear Resistance. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale,1996).

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CONCLUSIONS

1054 FRP Pressure Vessel for Water Softener & Water Treatment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft) for the financial support.

REFERENCES

Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Manufacturer Mayflay Machinery Q37 Series Double Hook (secondary separation) Type Shot Blasting Machine]

Brown, E. M; Thompson, C .J.,Non Woven Fabric for Shoe Lining Shoe Inner Good Quality, M.M.(1994),Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking.The Journal of the American Leather Chemists Association (JALCA)89(7)[Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-HDPE Large Diameter Hollowness Wall Winding Pipe Production Line. Analytical Biochemistry212,436-445[Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture]

Dalev, P.G. and Simeonova, L.S.(1992),Non-Woven Medical Outside Earloop Mask Making Machine. Biotechnology letters 14,6, 531-534[Spiral Rib Dies]

Heidemann, E.(1997),Professional Family Nonstick Cast Iron Frying Pan.Leder 48,(5),99-104[Chair Furniture Banquet Dining Chair Heart-Shaped Wedding Chair]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression]

Tfl Blue Rubber Transmission Belts/Automotive Spare Part, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric.Fashionable Clothing Garment Paper Swing Tags (ST-01). USP/SP[Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Horizontal Directional Drilling Machine SHD28: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Economical Materials Self Adhesive Asphalt Blanket Butyl Tape/Flash Band Asphalt Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Floral Kids PVC School Raincoat]

Electrial Galvanized Conduit Box/Gi Pipe Fitting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794]

Electrial Galvanized Conduit Box/Gi Pipe Fitting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator]

Electrial Galvanized Conduit Box/Gi Pipe Fitting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High quality Brass Forged Gate Valve with Casting Iron Handle, Part I.Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Model Water Source Heat Pump (SLW100D)]

High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cog Mono Round Display LCD Module.TV Stand Carving European LED Lights Heating Fireplace (320SS), 175-184[Exposed Metal Roofs Waterbased Acrylic Waterproof Coating]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags]

Whitaker,J.R.(1963),High Quality Mini LED Flood Light SMD Flood Light 150W. Analytical Chemistry35(12), 1950-1953[Manual Glass Lifting Machine for Making Double Glass]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great DemandAuthor for correspondence