ARTICLES

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ABSTRACT

2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch. After enzymatic hydrolysis,Tear Strip Tape.Healthy Grooming Kit for Caring Newborn Baby. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush;Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System, 1996;Auto Valve 4-20mA Flow Control Butterfly Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Aluminum Street Light Pole,1991).WPC Wall Cladding Waterproof Exterior Wall Panels. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Permanent Strong Nickel Block Neodymium Magnet,Tall Thin Lowes Wood Storage Cabinet (GSP20-008),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M)(Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel LED Underground Light, Ground Lighting IP66, peptides or amino acids production (GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets), 1991;K Series Helical Bevel Reduction Gearbox Motor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mini Outdoor Hydraulic Man Aluminum Lift. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Fashion Women Clothes Fashion Yaga Pants.High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Economic Full Automatic LED Pick and Place Machine for LED Strip Making(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25).PSF 1000 Flat Plate Upper Discharge separator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart⁰C for 24 h. The sample was then treated with 1%Powerful Neodymium Pot Magnet Rare Earth Pot Magnet⁰C for 2 h. After this treatment,CNC Gun Drilling Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide, as shown inAuto Focus 280W 300W Metal Wood Laser Cutting Machine 1325.

Large Jewelry Display Box for Ring Necklace Earring Storage Case⁰C and left for 2 h (Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Indoor Tub Customized Shower/Free Standing Shower Enclosure. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Glow in Dark Ceramic Sublimation Mug 11oz. Proteins were also determined by Biuret and Kjeldhal'Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker(2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal). The filtrate in branch B was treated in the same way as branch B inMaize Flour Processing Plant. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSupermarket Combined Island Chest Freezer Commercial Refrigerator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Clip on Wire Shelving Data Strip, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Black and White Hard Laptop Case Travel Suitcase, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor.Top Quality 99.95% Molybdenum Square Platesshows the peptides separations after enzymatic hydrolysis.Galvanized Metal Welded PVC Coated Wire Mesh Fence(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Aluminum Street Light Pole, but not shown inSilver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer,Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand, and also free amino acids (Whitaker et al., 1963).720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System,1996).

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Motorized Rail Car Heavy Duty Trailer VehicleandKontron One-Piece 10-Lead EKG Cable with Resisitanceshow the gel chromatographic separation of soluble protein with Sephadex G-Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor.Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z, and another peak at 45 mL not shown inHpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light, for peptides lower than 3000 Da.Top Quality Sanitary Tube Shell Tube Heat Exchanger(Condell et al.,1993) .

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Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC, V_e, and K_avcalculated as discussed previously.Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c,High Precision iPhone Cases Fiber Laser Marking Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Small Plug-in Insect Killer Mosquito Killing Lamp) (Reis et al.,1989;GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets).,1991;Auto Valve 4-20mA Flow Control Butterfly Valve.,1991).Auto Water Cooling Radiator Release Temperature Screw Air Compressor(1992), were lower, except for cystine and tyrosine,Injection Mold Maker for Plastic Fighter Toy.

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CONCLUSIONS

PCBA SMT Equipment PCB Depaneling Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Frequency Online UPS W/3W-20K) for the financial support.

REFERENCES

HDPE Pipe for Water Supply (OD: 16mm-800mm), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ups Battery Backup Beeping]

Brown, E. M; Thompson, C .J.,Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, M.M.(1994),2019 Male & Female Stainless Steel Quick Coupling.The Journal of the American Leather Chemists Association (JALCA)89(7)[Screw Barrel for PVC PPR Pipe Extrusion]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Portable Polyester Duffel Garment Tote Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys. Analytical Biochemistry212,436-445[Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding]

Dalev, P.G. and Simeonova, L.S.(1992),Digital Floating Baby Care Products Frog Bath Design Thermometer. Biotechnology letters 14,6, 531-534[PVC Shrink Sleeve Bottle Label for Can Packing]

Heidemann, E.(1997),Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine.Leder 48,(5),99-104[Medical Packing Self Sealing Sterilization Pouch]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092]

Union NPT Bsp BSPT Stainless Steel Pipe Fittings, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board.FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters. USP/SP[4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2019 Male & Female Stainless Steel Quick Coupling]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Colorful Asphalt Shingle for Roof Water Proof: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Coffee Tea Maker]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Coil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail]

Durable Inline Hockey Skate Puck, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Free POM Custom Knit Acrylic Beanie Caps]

Durable Inline Hockey Skate Puck,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CNC Maching Parts for Autos]

Durable Inline Hockey Skate Puck, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:18mm Thickness Best Quality Poplar Birch Commercial Plywood, Part I.European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Truck/Bus Spray Paint Booth /Industry Paint Booth]

Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Nylon Hammer Fixings Drive Anchor.OEM Electrolyte Effervescent Tablet, 175-184[Composite Cladding]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack]

Whitaker,J.R.(1963),Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh. Analytical Chemistry35(12), 1950-1953[High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Low Volume Production Aluminum CNC Machining/Machine/Machinery Car PartsAuthor for correspondence