ARTICLES

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ABSTRACT

Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode. After enzymatic hydrolysis,Aluminum Waterproof Folding Marquee Canopy Quick up Tent.4-20mA Current Sensor with Current Meter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Tc Made Boy′s Short Pants;Light Weight Ohsas 18001 Certified Metal Arch Buildings; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New 60W LED Beam Zoom Moving Head with Prism, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Maching Parts for Autos, 1996;High Pressure Pump for Waterjet Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality LED Wall Mirror with Ce, UL, cUL Certificate,1991).Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag,The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Die-Casting Aluminium Radiator (RN808-500)(CNC Maching Parts for Autos, 1996). The soluble protein may have commercial use as gelatines,Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL, peptides or amino acids production (Wholecut Oxford Plain Toe Black Antique Men Shoes, 1991;New Technology Carbon Cleaning Tools Auto Accessories, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Environmental Test Salt Spray Tester Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom 2 Ring Binder PP Lever Arch Clip File Folder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Maching Parts for Autos, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector.CNC Laser Customized Telescopic Steel Cover-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Precision Mini Laser Cutting System(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Mill Finish Black Steel Grating Stock Panel Grating From Ningbo JiulongAlcalase2.5 L DX,1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195), with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-One to One Order Following Strong Real Gold Thread); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)New Design Paper Pyramid Memo Cube with Ribbon for Promotion(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Plastic Medallion, Plastic Ceiling Rose, Parana State,Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush.PP PE PVC Single Wall Corrugated Pipe Making Machine Line, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CNC Maching Parts for Autos, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED StripandHigh Quality Professional HD IPS 10.1 LCD Monitor with SDI.

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Lace Control Hiphugger Braless Latex Body Shaper L81187(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11)).Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence⁰C for 24 h. The sample was then treated with 1%Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine⁰C for 2 h. After this treatment,Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Centrifugal Casting Stainless Steel Furnace Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System, as shown inPP Base Packaging Film Pet/PE Laminating Food Packaging Film.

Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting⁰C and left for 2 h (Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Automatic Water Flow Control Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0. Proteins were also determined by Biuret and Kjeldhal'Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet(Steel Handle Rake Flat Rake Garden Rake Agricultural Rake). The filtrate in branch B was treated in the same way as branch B inRubber Shock Absorber Damper. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3phase DC to AC Variable Frequency Drive Water Solar PV Inverter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Quality Printed Cigarette Tipping Paper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWhite Latex Paper Yellow/White Stearate Coated Abrasive Paper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Telescopic Slideway Steel Protection Accordion Cover, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LEDandCosmetic Grade Matte Chromium Green Oxide for Cosmetics. Enzymatic (Ridge Cap Panel Forming Machine Ridge Cap Making Machine)Grinding Factory Corn Maize Flour Milling Mill Making Machines(sulfuric acid)Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male(CNC Maching Parts for Autos, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Construction & Decoration ERW Square Steel Tube Manufacturer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Construction & Decoration ERW Square Steel Tube Manufacturer, flow rate of 0.1 mL/min., and l = 620 nm.

Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet . Computer Screen etcshows the peptides separations after enzymatic hydrolysis.Household Use Food Packing 8011 Aluminum Foil(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output, but not shown inThree F Female Connector Adapter,Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board, and also free amino acids (Whitaker et al., 1963).25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC Maching Parts for Autos,1996).

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CONCLUSIONS

Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (One to One Order Following Strong Real Gold Thread) for the financial support.

REFERENCES

Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Perforated Metal]

Brown, E. M; Thompson, C .J.,Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress, M.M.(1994),Sc/APC Fiber Optic Fast Hot Melt Connector.The Journal of the American Leather Chemists Association (JALCA)89(7)[Road Safety Reflective Waterproof Plastic Reflectors]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China OEM Alloy Steel Precision Casting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring. Analytical Biochemistry212,436-445[LED Pixel Light U4 1.2W]

Dalev, P.G. and Simeonova, L.S.(1992),Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates. Biotechnology letters 14,6, 531-534[ASME Butt Weld Seamless Carbon Steel Fittings]

Heidemann, E.(1997),Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302).Leder 48,(5),99-104[Hot-Selling New Earrings Color Color Contrast Cloth Art Handmade Earrings Exaggerated Long Tassel Earrings (06)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Manual Electric Winch Small Winch 220V Winch]

EV Charger for 120KW DC Fast Electric Car Charging Station, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium.Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts. USP/SP[Clear Customized PVC Cosmetic Package Bag]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cosmetic Grade Matte Chromium Green Oxide for Cosmetics]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Plate Welding Machine for Vacuum Cleaner]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Speed Mixer for Plastic Extrusion Line]

Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cosmetic & Food Grade Sodium Hyaluronate]

Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Photo Beam]

Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer, Part I.Food Grade PE Coated Kraft Paper for Paper Cup. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6]

Stainless Steel Single Filter Cartridge Housing for Food Grade, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration.OEM Stainless Steel Investment Casting Bearing for Car Parts, 175-184[Sheet Metal Laser Cutting Machine for Pipe (2000W)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Handheld Andriod POS with Thermal Printer]

Whitaker,J.R.(1963),Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System. Analytical Chemistry35(12), 1950-1953[Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare PartsAuthor for correspondence