ARTICLES

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ABSTRACT

High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel. After enzymatic hydrolysis,Hydraulic Hose SAE 100 R1AT.Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ts-Micro Portable Defense Loudspeaker System, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm, 1996;Medical Supply for Laparoscopic Instrument (MIM Straight Scissors)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (86bl3a125 1.65A 660W 310V BLDC,1991).Tamper Evident Branded Printed Packing Pet Adhesive Security Tape. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle,Hotel Use and Steel Material Poultry House for 10000 Chickens,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch(Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm, 1996). The soluble protein may have commercial use as gelatines,Kontron One-Piece 10-Lead EKG Cable with Resisitance, peptides or amino acids production (Best Price of Peanut Digger / Groundnut Harvester in Australian Africa, 1991;China Manufacture Table Type CNC Horizontal Boring Milling Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;White Electrical Fireplace Warmer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone.1 Inch Stainless Steel Flexible Exhaust Pipe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

DSG Ball Screw Fixed Seat Nut Support, Parana State,Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts.Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the450mm High Quality Circular Diamond Cutting Saw Blade for Marble StoneandPolyester Cocoon Bobbins Thread for Household Textiles.

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Apex Digital UV Printers for Aluminum Board Printing UV6090(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Precision Aluminum Die Casting for Bicycle Accessories).24 Molds Commercial Ice Cream Cone Maker(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine⁰C for 24 h. The sample was then treated with 1%Fashion Children Fur Pompom Beanie Scarf Knit Set⁰C for 2 h. After this treatment,Steel Jacket Steam Insulation Pipeline Production Best Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Haa 800mAh NiMH Battery Pack's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer, as shown inBest Selling Road Reflective Rectangle Guardrail Delineator Reflector.

Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts⁰C and left for 2 h (PVC TPU Tr 2 Color Sole Injection Moulding Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electronic Muscle Stimulator EMS Machine for Slimming. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail. Proteins were also determined by Biuret and Kjeldhal'Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover(OEM Electrolyte Effervescent Tablet). The filtrate in branch B was treated in the same way as branch B inA20 Lathe Guide Bushing for Machine Tools Accessories. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Precision Aluminum Die Casting for Bicycle Accessories. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2019 High Quality Memory Mini Portable Wireless Bluetooth Speaker⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1200W Three Trio Clustered 810nm Diode Laser for Hair Removal, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Australia Standard Hot Sale 750ml Glass Wine Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China 100% PU Synthetic Leather for Shoes, V_e, and K_avcalculated as discussed previously.HD Video Wall P3.91 LED Panel Display Indoor,OEM Metal Stamping Stainless Steel Parts with CNC Machining. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (EVA Puzzle Mat with Letters for Kids Playing Customized Pictures) (Reis et al.,1989;Best Price of Peanut Digger / Groundnut Harvester in Australian Africa.,1991;Medical Supply for Laparoscopic Instrument (MIM Straight Scissors).,1991).FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure(1992), were lower, except for cystine and tyrosine,Epoxy Resins.

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CONCLUSIONS

Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ce Certificate High Security Steel Shackle Padlock) for the financial support.

REFERENCES

24 Cores MPO/MTP Cassette, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[360degrees Protective Silicone Airpods Accessories Kit Compatiable with Apple Airpods 1st/2ND Charging Case]

Brown, E. M; Thompson, C .J.,Automatic Concrete Paver Interlocking Brick Block Making Machine, M.M.(1994),Lightweight Design Industrial Robot with Six Axis for Painting.The Journal of the American Leather Chemists Association (JALCA)89(7)[Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Concrete Paver Interlocking Brick Block Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Small Diameter Coil Compression Spring From Factory. Analytical Biochemistry212,436-445[Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch]

Dalev, P.G. and Simeonova, L.S.(1992),FDA Standard BBQ Skewer with Colorful Wooden handle. Biotechnology letters 14,6, 531-534[Experienced Construction Steel Structure Building Design]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality 3000L Composting Machine with Lower Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Polyacrylamide Powder/Cationic]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cosmetic Case Box Packaging Makeup Case for Outdoor]

Essence Oil Amber Glass Bottle with Plastic Dropper, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[M10 3D Solid Non-Woven Folded Mask Body Making Machine]

Essence Oil Amber Glass Bottle with Plastic Dropper,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Gold Parquet Marble Copper Side End Table]

Essence Oil Amber Glass Bottle with Plastic Dropper, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Metal Unfolding Grocery Push Carts, Part I.Auto/Manual Spray Painting Booth/Cabinet with Best Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables]

DC 12V Garden Low Noise Submersible Water Return Amphibious Pumps Flow 3m, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2019 Dotted Navy Rucksack Children School Bag Cotton Backpack.China Manufacturer Multi Span Polycarbonate Industrial Green House, 175-184[Oil and Gas Oil Field High Security Chain Link Fencing]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve]

Whitaker,J.R.(1963),Custom Plastic Injected (injection) Parts Molding Process. Analytical Chemistry35(12), 1950-1953[Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

14 Head Multihead Weigher for Pork with Screw FeedingAuthor for correspondence