ARTICLES

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ABSTRACT

Best Price Spc Lvt PVC Vinyl Flooring for Home Office. After enzymatic hydrolysis,Red Papillon (JSY-3) Satin Ribbon Flower.Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Steel Structure Construction Building Metal House;1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Sanitary Thread Pneumatic Butterfly Valve, 1996;2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Design Slotted Board MDF for Wall Panel,1991).Factory Wholesale Reach Standard Home and Office Furniture Fitting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing,Hot Sale Energy Saving Induction Heater for Billet Making,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Electric 154 Stone Polisher 17inch Floor Cleaning Machine(Stainless Steel Sanitary Thread Pneumatic Butterfly Valve, 1996). The soluble protein may have commercial use as gelatines,Hydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic, peptides or amino acids production (2019 New Nylon Material Rapid Prototype Manufacturer in China, 1991;Stainless Steel Pin Custom Stamping, Punch Punching Parts, Stamping Metal Parts, Brass Stamping, Electrical Stamping, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Portable Polyester Duffel Garment Tote Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Sanitary Thread Pneumatic Butterfly Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Decoration Sign Green Grey White PVC Foam Board.3kw 4kw 5kw off Grid Solar Inverter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3 Layer Film Blowing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive).8FT Wood Log Debarker Machine for Plywood Machinery(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine⁰C for 24 h. The sample was then treated with 1%Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine⁰C for 2 h. After this treatment,High Density Graphite Crucible for Melting Metal. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hydraulic Press Corrugated Cold Roll Automatically Forming Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine, as shown inHigh Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing.

Medical Supplier Non Woven 3ply Earloop Disposable Face Mask⁰C and left for 2 h (Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat. Proteins were also determined by Biuret and Kjeldhal'High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087)(Hot Dipped Galvanized Deer Fence From Factory). The filtrate in branch B was treated in the same way as branch B inHigh Quality Deer Fence/Wire Mesh Sheep Fencing/Security Fence. The final soluble protein shown were used for peptides separations using gel permeation chromatography,LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.19gauge Galvanized Twin Binding Wire⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/6.35mm Audio Connector Plug Jack, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CroissantandStainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater. Enzymatic (Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62)Motar Plastering Machine with Screw Pump(sulfuric acid)OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126)(Stainless Steel Sanitary Thread Pneumatic Butterfly Valve, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor, flow rate of 0.1 mL/min., and l = 620 nm.

Fr4 Copper Clad Laminate PCB Manufacture Company.100% Durable Flooring Roll for Hotel Office School and Health Project Floorshows the peptides separations after enzymatic hydrolysis.Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUL Certified 12V 277VAC Smart Relay for Utility Meters, but not shown inBeer Made in China OEM Abv 8.0%Vol Lager Beer,Ibutamoren Mesylate Mk-677 for Healing Injuries Sarms, and also free amino acids (Whitaker et al., 1963).Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Sanitary Thread Pneumatic Butterfly Valve,1996).

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CONCLUSIONS

ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pet Material Alien 9654 Wet Inlay UHF Windshield Label) for the financial support.

REFERENCES

High Performance Alcohol Pad Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Expansion Alloy Tube]

Brown, E. M; Thompson, C .J.,Ss304 Sanitary Welded Butterfly Valve, M.M.(1994),2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plug-in Assembly Type Straight Through Joint and Insulation Joint]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Anti-Abrasion PVC Artificial Leather for Car Seat (128#). Analytical Biochemistry212,436-445[304 Seamless Stainless Steel Tube]

Dalev, P.G. and Simeonova, L.S.(1992),Digital Control with LED Display DC Solar Small Deep Freezer Fridge. Biotechnology letters 14,6, 531-534[500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory]

Heidemann, E.(1997),Automatic Economical Check Weigher with High Speed.Leder 48,(5),99-104[Aluminum Laser Engraving Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Canvas Casual Men Shoes]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Kraft Paper Laminated Aluminum Foil]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Small Tea Bag Packing Machine Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Silicone Rubber Membrane Keyboard for Controlled Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts]

Carbon Dioxide Gas with 50L Seamless Cylinder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),UV8d Wouxun High Gain Antenna Dual Band Antenna-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator]

Carbon Dioxide Gas with 50L Seamless Cylinder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine]

Carbon Dioxide Gas with 50L Seamless Cylinder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire, Part I.Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Good Quality High Precision Machined Milling CNC Brass Parts]

Swbf 7b2s Bolt Former, Bolt Machine, Cold Forging Machine, Cold Heading Machine, Parts Former, Nut Tapping Machine, Threadrolling Machine, Automatic Bolt Making, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Fashion Logo Sport Rubber Silicone Bracelet Customized Printed Smart RFID Watch USB Mosquito Imprinted Embossed Silicon Wristband for Promotional Gift.Smiley Canvas School Backpack for Teenagers Girls, 175-184[China Hot Sell Crawler Type Shot Blasting Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lightweight Design Industrial Robot with Six Axis for Painting]

Whitaker,J.R.(1963),P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen. Analytical Chemistry35(12), 1950-1953[Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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