ARTICLES

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ABSTRACT

High Brightness 100lm/W 45W CREE COB LED Track Light. After enzymatic hydrolysis,Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control.Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fiber Reinforced Plastic GRP FRP Fiberglass Gratings;Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Skytone 4 Channel Digital 1u Light Weight Power Amplifier, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3, 1996;Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp,1991).Mu Optical Fiber Connectors for Cable Assembly. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning,Best Price 201 304 Stainless Steel Coils,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,National Day Holiday Football Bravo Hand Weaving Flag(Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3, 1996). The soluble protein may have commercial use as gelatines,Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine, peptides or amino acids production (New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function, 1991;2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM Customized Logo Print Polyester Nylon Gym Sport Blue Drawstring Backpack Bag Supplier.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Garden Irrigation Spray Micro Sprinkler Controller. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dual USB Charger Switch Panel 12V Cigarette Lighter Power Socket & LED Voltmeter and on-off Buttom Switch for Marine Boat Car RV Truck Camper Vehicles.Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Free POM Custom Knit Acrylic Beanie Caps(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine).40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pillow Case Automatic Sewing Machine Bc901⁰C for 24 h. The sample was then treated with 1%Aluminum Lamp Body 90mm Cut out Square LED COB Downlights⁰C for 2 h. After this treatment,Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Metal Tin with Clear Lid's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ouwave China Factory Wholesale Air Purifier Smart Scent Machine, as shown inStandard Disposable Oxygen Mask/Nebulizer Mask with Tubing.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlow Packing Machine for Bread, Soap, Biscuit, Chocolate(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor LED Decorative Street Linear Wall Lighting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on SaleandGood Quanlity of Ue319an20z Hydraulic Oil Filter Element. Enzymatic (Universal Manual Turret Milling Machine(Mill X6336))Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries(sulfuric acid)Hexagonal Netting/ Gabion Box/ Gabion Basket(Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Proper Price Good Quality 10ml 20ml Airless Pump Bottle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Proper Price Good Quality 10ml 20ml Airless Pump Bottle, flow rate of 0.1 mL/min., and l = 620 nm.

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Bsp 60° Male Cone Seat Carbon Steel Fittings, V_e, and K_avcalculated as discussed previously.Soccer Field Artificial Grass Cutting Machine for Installation,Plastic Nylon Sheet with High Temperature. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Super Soft 100% Viscose From Bamboo Bedding Set) (Reis et al.,1989;New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function.,1991;Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic.,1991).PVC TPU Tr 2 Color Sole Injection Moulding Machine(1992), were lower, except for cystine and tyrosine,Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors.

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CONCLUSIONS

Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803) for the financial support.

REFERENCES

Corrugated Paper Honeycomb Core Board for Doors, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2-10t/D Coconut Oil Refining Unit]

Brown, E. M; Thompson, C .J.,Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture, M.M.(1994),Wood Handle Carbon Steel Sickle S202.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Electric PCB Circuit Board with High Quality]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Custom Golden Colored Powder Pill Organizer. Analytical Biochemistry212,436-445[Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry]

Dalev, P.G. and Simeonova, L.S.(1992),Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket. Biotechnology letters 14,6, 531-534[Bitumen Waterproof Membrane Minerla Surface Waterproof Material]

Heidemann, E.(1997),Disposable Pipette Tip 40~200UL with Ce.Leder 48,(5),99-104[Cylinder Magnet With Hole]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Centrifugal Casting Stainless Steel Furnace Tube]

125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale.1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids. USP/SP[Non Woven Fabric for Shoe Lining Shoe Inner Good Quality]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Xtsky Automotive Wheel Hub Bearing (DAC37720037)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-86 Type WiFi Smart Touch Switch Controll by Tuya APP: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Low Profile Sp-Level Linear Rail for 3D Printer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Price Aws E6013 Welding Electrode]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure]

100% Wool Felt Wide Brim Fedora Trilby Hat, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Super Handy Home & Office & Hotel Facial Tissue Paper-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aluminum Sheet Metal Parts Custom Fabrication]

100% Wool Felt Wide Brim Fedora Trilby Hat,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sc/APC Fiber Optic Fast Hot Melt Connector]

100% Wool Felt Wide Brim Fedora Trilby Hat, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep, Part I.Hidden Handle Safe for Children in Bathroom Cabinet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bsp 60° Male Cone Seat Carbon Steel Fittings]

Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets.Transformer Dehydrate Breather Casing (organgle silicon gel), 175-184[125kHz RFID Hotel Door Lock Card with T5577/Atmel5577 Chip]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting]

Whitaker,J.R.(1963),3 Full HD 1080P Mini Car DVR Dashcam Camera. Analytical Chemistry35(12), 1950-1953[Industrial Low Temperature Water Cooled Scroll Water Chiller]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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